| 赵云超,宋颖.皮肤鳞状细胞癌组织微 RNA-103a-2-5p、钙黏附蛋白 11表达及其临床意义[J].安徽医药,2024,28(7):1372-1377. |
| 皮肤鳞状细胞癌组织微 RNA-103a-2-5p、钙黏附蛋白 11表达及其临床意义 |
| Expression and clinical significance of miR-103a-2-5p and CDH11 in cutaneous squamous cell carcinoma |
| |
| DOI：10.3969/j.issn.1009-6469.2024.07.022 |
| 中文关键词: 皮肤鳞状细胞癌 微 RNA-103a-2-5p 钙黏附蛋白 11 癌组织 预后 |
| 英文关键词: Cutaneous squamous cell carcinoma MiR-103a-2-5p CDH11 Cancer tissue Prognosis |
| 基金项目:陕西省卫生健康科研项目( 2022D015) |
|
| 摘要点击次数: 401 |
| 全文下载次数: 289 |
| 中文摘要: |
| 目的分析皮肤鳞状细胞癌组织中微 RNA-103a-2-5p(miR-103a-2-5p)和钙黏附蛋白 11(CDH11)的表达水平，探讨二者在临床研究中的意义。方法选取 2017年 1月至 2019年 6月宝鸡市人民医院诊治的 98例皮肤鳞状细胞癌病人为研究对象，收集病人术中切除的癌组织及癌旁正常组织。采用实时荧光定量 PCR(qRT-PCR)法检测组织中 miR-103a-2-5p的表达水平。采用酶联免疫吸附(ELISA)法检测 CDH11表达水平。 TargetScanHuman网站预测 miR-103a-2-5p与 CDH11的靶向关系； Pearson相关性分析癌组织中 miR-103a-2-5p和 CDH11水平的关系； Kaplan-Meier生存曲线分析 miR-103a-2-5p、CDH11表达与皮肤鳞状细胞癌病人预后的关系；影响皮肤鳞状细胞癌病人预后的因素采用 Cox回归分析；受试者操作特征曲线(ROC曲线)分析 miR-103a-2-5p和 CDH11的表达对皮肤鳞状细胞癌的诊断价值。结果与癌旁正常组织［1.03±0.12，(5.06±1.43)μg/L］相比，皮肤鳞状细胞癌组织 miR-103a-2-5p表达水平(1.46±0.38)显著升高(P<0.05)，CDH11表达水平［(2.96±0.62)μg/L］明显降低(P<0.05)； TargetScanHuman网址预测 miR-103a-2-5p与 CDH11间存在结合位点；经 Pearson相关性分析， miR-103a-2-5p与 CDH11水平呈负相关( P<0.05)；两者均与病人病理分级、 TNM分期、淋巴结转移、侵袭程度相关(P<0.05)； miR-103a-2-5p高表达组和 CDH11低表达组复发率显著高于 miR-103a-2-5p低表达组和 CDH11高表达组(P<0.05)； TNM分期、淋巴结转移、 miR-103a-2-5p是影响病人预后的危险因素(P<0.05)CDH11是影响病人预后的保护因素(P<0.05)； miR-103a-2-5p、CDH11二者联合诊断皮肤鳞状细胞癌的 ROC曲线下面积(AUC)0.836，显著优于其各自单独诊断(P=0.018、0.021)。结论皮肤鳞状细胞癌病人 miR-103a-2-5p表达水平升高， 为， |
| 英文摘要: |
| Objective To analyze the expression levels of microRNA-103a-2-5p (miR-103a-2-5p) and cadherin 11 (CDH11) in skin squamous cell carcinoma (SCC), and to explore their clinical significance.Methods Ninety-eight patients with skin squamous cell carcinoma diagnosed and treated in Baoji People's Hospital from January 2017 to June 2019 were selected as the study objects, and thecancerous tissues and normal tissues adjacent to the cancer were collected. The miR-103a-2-5p level in tissues was detected by real-time fluorescent quantitative PCR (qRT-PCR). The expression of CDH11 was detected by enzyme-linked immunosorbent assay (ELISA). TargetScanHuman website predicted the target relationship between miR-103a-2-5p and CDH11. Pearson correlation analysis was performed on the expression levels of miR-103a-2-5p and CDH11 in cancer tissues. Kaplan-Meier survival curve was used to analyze the relationship between miR-103a-2-5p, CDH11 expressions and prognosis of patients with cutaneous squamous cell carcinoma(CSCC); the factors affecting prognosis of patient with SCC were analyzed using Cox regression analysis; the diagnostic value of miR103a-2-5p and CDH11 expressions in CSCC was analyzed by ROC curve.Results Compared with normal tissue adjacent to cancer [miR-103a-2-5p: 1.03±0.12, CDH11: (5.06±1.43) μg/L], the expression level (1.46±0.38) of miR-103a-2-5p in skin SCC tissue was significantly increased (P<0.05), while the expression level [(2.96±0.62) μg/L] of CDH11 was lower (P<0.05). TargetScanHuman website predicted that there was a binding site between miR-103a-2-5p and CDH11. There was a negative correlation between miR-103a-2-5p and CDH11 expression (P<0.05), both of which were correlated with pathological grade, TNM stage, lymph node metastasis and invasion degree (P<0.05). The recurrence rate was significantly higher in the miR-103a-2-5p high-expression group and the CDH11 low-expression group than in the miR-103a-2-5p low-expression group and the CDH11 high-expression group (P<0.05); TNM stage, lymph node metastasis, and miR-103a-2-5p were risk factors affecting the prognosis of patients (P<0.05), and CDH11 was a protective factor affecting the prognosis of patients (P<0.05); the area under the ROC curve (AUC) of the combination of miR-103a-2-5p and CDH11 in the diagnosis of CSCC was 0.836, which was obviously better than their respective diagnosis alone (P=0.018, 0.021).Conclusions The expression level of miR-103a-2-5p is increased and the expression level of CDH11 is decreased in patients with CSCC. Both of them areclosely related to the clinicopathological characteristics and prognosis of patients, and the combination of the two can better diagnoseCSCC. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
