| 宋家木,任书伟,丁小崇,等.三阴性乳腺癌组织中微 RNA-331-3p和跨膜蛋白 119水平变化与临床预后的相关性[J].安徽医药,2024,28(8):1582-1586. |
| 三阴性乳腺癌组织中微 RNA-331-3p和跨膜蛋白 119水平变化与临床预后的相关性 |
| Correlation between the changes of miR-331-3p and TMEM119 levels and clinical prognosis in triple negative breast cancer |
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| DOI：10.3969/j.issn.1009-6469.2024.08.020 |
| 中文关键词: 三阴性乳腺癌 微小 RNA-331-3p 跨膜蛋白 119 预后 |
| 英文关键词: Triple negative breast neoplasms MicroRNA-331-3p Transmembrane protein 119 Prognosis |
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| 中文摘要: |
| 目的探究三阴性乳腺癌( TNBC)组织中微 RNA-331-3p(miR-331-3p)和跨膜蛋白 119(TMEM119)水平变化与临床预后的相关性。方法选取 2017年 1月至 2019年 1月在信阳市中心医院行手术治疗的 98例 TNBC病人的癌组织以及癌旁组织，分别作为 TNBC组和癌旁组。在术后 3年对所有病人进行随访，分为预后良好组为 23例，预后不良组为 75例。通过实时荧光定量 PCR(qRT-PCR)法检测 miR-331-3p和 TMEM119的表达水平；采用 Pearson法分析 TNBC组织中 miR-331-3p和 TMEM119表达水平的相关性；分析 miR-331-3p和 TMEM119水平与 TNBC病人预后的关系；影响 TNBC病人预后的影响因素采用 Cox回归的方法进行分析。结果 TNBC组 TMEM119(1.53±0.24)的表达水平与癌旁组相比( 1.01±0.13)显著上升， miR-331-3p(0.74±0.13)的表达水平与癌旁组( 1.02±0.11)相比显著下降( t=41.53、41.53，P<0.05)。 Pearson相关性分析结果显示， miR-331-3p和 TMEM119表达水平呈负相关关系( r=-0.49，P<0.001)。 miR-331-3p和 TMEM119表达水平与肿瘤长径、 TNM分期、腋窝淋巴结转移有关( P<0.05)。 miR-331-3p高表达组病人 3年内生存率 16/49(32.65%)高于低表达组 7/49(14.29%)； TMEM119高表达组病人 3年内生存率 4/49(8.16%)低于低表达组 19/49(38.78%)(Z=4.60，P=0.032；Z=12.78，P<0.001)。预后不良组 miR-331-3p |
| 英文摘要: |
| Objective To explore the correlation between the changes of microRNA-331-3p s(miR-331-3p) and transmembrane pro- tein 119 (TMEM119) levels in triple negative breast cancer (TNBC) tissues and clinical prognosis.Methods The cancerous tissues and adjacent tissues of 98 patients with TNBC who underwent surgical treatment in Xinyang Central Hospital from January 2017 to Jan-uary 2019 were selected as TNBC group and adjacent group respectively. All patients were followed up 3 years after surgery, and weredivided into good prognosis group (23 cases) and bad prognosis group (75 cases).The expression levels of miR-331-3p and TMEM119 were detected by real-time fluorescent quantitative PCR (qRT-PCR); Pearson method was used to analyze the correlation between miR331-3p and TMEM119 expression levels in TNBC tissues;The relationship between the levels of miR-331-3p and TMEM119 and theprognosis of TNBC patients was analyzed. The factors influencing the prognosis of TNBC patients were analyzed by Cox regressionmethod.Results The expression level of TMEM119 (1.53±0.24) in the TNBC group was significantly increased compared with that in the para-cancer group (1.01±0.13), and the expression level of miR-331-3p (0.74±0.13) was significantly decreased compared with that in the para-cancer group (1.02±0.11) (t=41.53, 41.53, P<0.05). Pearson correlation analysis showed that there was a negative correla- tion between miR-331-3p and TMEM119 expression levels (r=-0.49,P<0.001. The 3-year survival rate of patients with high expression of miR-331-3p was 16/49 (32.65%) higher than that of patients with low expression of Mir-331-3p was 7/49 (14.29%).The 3-year surviv-al rate of TMEM119 patients in the high expression group was 4/49 (8.16%) lower than that in the low expression group 19/49 (38.78%)(Z=4.60,P=0.032; Z=12.78, P<0.001). The expression level of miR-331-3p (0.65±0.13) in the poor prognosis group was significantlylower than that in the good prognosis group (1.03±0.12), and the expression level of TMEM119 (1.69±0.27) was significantly higherthan that in the good prognosis group (1.02±0.14) (t=38.74,33.30, P<0.05).The results showed that the expression levels of TNM stage(HR=5.88), miR-331-3p (HR=2.45) and TMEM119 (HR=2.86) were risk factors affecting the prognosis of TNBC patients (P<0.05).Con- clusion The expression of miR-331-3p is low and TMEM119 is high in cancer tissues of TNBC patients, and the levels of both areclosely related to prognosis assessment. |
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