| 秦万,郭生琼,欧维正,等.贵州省某专科医院非结核分枝杆菌的菌种鉴定及耐药检测分析[J].安徽医药,2024,28(8):1608-1612. |
| 贵州省某专科医院非结核分枝杆菌的菌种鉴定及耐药检测分析 |
| Identification and drug resistance analysis of nontuberculous mycobacteria in a specialized hospital in Guizhou province |
| |
| DOI：10.3969/j.issn.1009-6469.2024.08.026 |
| 中文关键词: 非结核分枝杆菌 菌种鉴定 药物敏感试验 微量肉汤稀释法 基因芯片法 |
| 英文关键词: Nontuberculous mycobacteria Strain species identification Drug susceptibility test Microbroth dilution method Gene chip |
| 基金项目:贵州省科技厅基础研究计划(黔科合基础〔 2020〕1Y355) |
|
| 摘要点击次数: 519 |
| 全文下载次数: 383 |
| 中文摘要: |
| 目的探讨非结核分枝杆菌( NTM)的菌种鉴定分布及耐药性，为临床诊治提供参考依据。方法搜集 2017―2020年贵州某专科医院微量肉汤稀释法药敏试验对 -硝基苯甲酸( PNB)培养基阳性的疑似 NTM菌株，经聚合酶链式反应( PCR)-荧光探针法初步鉴定为 NTM后，用基因芯片法进行菌种鉴定，采用微量肉汤稀释法进行 15种药物测试。结果贵州地区流行的 100例 NTM菌株经鉴定分为 10种菌型类别。其中，占比最高者为龟 /脓肿分枝杆菌 48%(48/100)其后依次为鸟分枝杆菌 16%(16/100)，堪萨斯分枝杆菌 12%(12/100)，胞内分枝杆菌 11%(11/100)。药敏结果显示 100株 NTM总，耐药率 61.8%，龟/脓肿分枝杆菌耐药率最高 76.4%，其后依次为微黄分枝杆菌 73.3%、偶然分枝杆菌 55.6%、鸟分枝杆菌 53.8%和戈登分枝杆菌 53.3%。男女病人比例为 63∶37，男性耐药率 56.1%；女性耐药率 71.5%。结论贵州地区有 10种 NTM菌种流行，龟/脓肿分枝杆菌是该地区 NTM优势菌种。不同菌种间耐药率差异较大，龟/脓肿分枝杆菌耐药率最高。男性病人多于女性，女性耐药率高于男性。应用快速 NTM菌种鉴定和药敏试验，可为 NTM防控项目的决策者提供参考依据，有助于促进医务人员对抗 NTM药物的科学选择。 |
| 英文摘要: |
| Objective To explore the identification, distribution and drug resistance of nontuberculous mycobacteria, and to providereference for clinical diagnosis and treatment. Methods Suspected NTM strains with positive PNB culture medium were collectedfrom a specialized hospital in Guizhou from 2017 to 2020. After being preliminarily identified as NTM by PCR-fluorescence probe method, the strains were identified by gene chip method, and 15 drugs were tested bybroth microdilution method.Results The 100 NTM strains circulating in Guizhou were classified into 10 types, of which the highest proportion was 48% (48/100) of M.chelonae /M. bscessus, followed by M.avium 16% (16/100), M. Kansasii 12% (12/100), M.intracellulare 11% (11/100). The results of drug sensitivityshowed that the total drug resistance rate of 100 NTM strains was 61.8%, and the highest drug resistance rate was 76.4%, followed byMycobacterium flavum 73.3%, Mycobacterium fortuitum 55.6%, Mycobacterium avium 53.8% and Mycobacterium Gordon 53.3%. The ratio of male to female patients was 63∶37, and the male drug resistance rate was 56.1%. The female drug resistance rate was 71.5%. Conclusions There are 10 kinds of NTM strains prevalent in this area, and Mycobacterium tortoise/abscess is the dominant NTM strain in this area. The drug resistance rate varies greatly among different strains, and the drug resistance rate of Mycobacterium chelonei/Ab- scess is the highest. There are more male patients than female, and the drug resistance rate of female is higher than that of male. The ap-plication of rapid NTM strain identification and drug sensitivity test can provide reference for the decision makers of NTM preventionand control projects, and help to promote the scientific choice of anti-NTM drugs for medical staff. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
