| 李铃铃,尹翼,何爱琴.卵巢浆液性肿瘤组织中转导蛋白 β样 1X相关蛋白 1、睾素 2表达对病人的预后价值[J].安徽医药,2024,28(9):1761-1766. |
| 卵巢浆液性肿瘤组织中转导蛋白 β样 1X相关蛋白 1、睾素 2表达对病人的预后价值 |
| Prognostic value of transducin β-like 1X-related protein 1 and testosterone 2 expression in ovarian serous tumor tissue in patients |
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| DOI：10.3969/j.issn.1009-6469.2024.09.015 |
| 中文关键词: 卵巢肿瘤 转导蛋白 β样 1X相关蛋白 1 睾素 2 预后 |
| 英文关键词: Ovarian neoplasms Transducin β-like 1X-related protein 1 Testosterone 2 Prognosis |
| 基金项目:江苏省南通市科技计划项目( MS22022007) |
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| 中文摘要: |
| 目的探究卵巢浆液性肿瘤组织中转导蛋白 β样 1X相关蛋白 1(TBL1XR1)、睾素 2蛋白表达水平及其与预后的关系。方法选取 2015年 4月至 2017年 4月南通大学附属肿瘤医院 102例卵巢浆液性癌病人(恶性组)50例卵巢交界性浆液性肿瘤病人(交界组)48例卵巢良性浆液性肿瘤病人(良性组)。免疫组织化学法检测组织中 TBL1XR1、，睾素 2蛋白表达。实时荧光定量 PCR检测血，清 TBL1XR1、睾素 2 mRNA表达水平。 Kaplan-Meier分析 TBL1XR1、睾素 2蛋白不同表达情况与生存时间关系。 Cox回归模型分析恶性组病人不良预后影响因素。受试者操作特征曲线( ROC曲线)分析血清 TBL1XR1、睾素 2 mRNA对恶性组不良预后预测价值。结果恶性组 TBL1XR1、睾素 2蛋白高表达率高于交界组，交界组又高于良性组( P<0.05)。恶性组、交界组、良性组血清 TBL1XR1(2.27±0.58，1.41±0.37，1.06±0.13)、睾素 2 mRNA表达水平( 2.66±0.67，1.58±0.40，1.09±0.15)逐次降低( P<0.05)。 FIGO分期为 Ⅲ~Ⅳ期、淋巴结转移及组织学分级为高级别 TBL1XR1、睾素 2蛋白高表达率高于 Ⅰ~Ⅱ期、淋巴结无转移及低级别( P<0.05)。生存分析显示， TBL1XR1、睾素 2蛋白高表达组 5年累积生存率分别低于 TBL1XR1、睾素 2低表达组( P<0.05)。 Cox多因素回归分析， FIGO分期 Ⅲ~Ⅳ期、淋巴结发生转移、组织学分级为高级别、 TBL1XR1高表达、睾素 2高表达是卵巢浆液性癌病人不良预后危险因素( P<0.05)。恶性组死亡病人血清 TBL1XR1(2.65±0.61比 1.82±0.54)、睾素 2 mRNA表达水平( 3.10±0.76比 2.15±0.57)高于生存病人( P<0.05)。 ROC曲线结果显示，血清 TBL1XR1、睾素 2 mRNA预测恶性组病人不良预后的曲线下面积( AUC)都为 0.84；二者联合预测不良预后的 AUC为 0.94，优于 TBL1XR1、睾素 2 mRNA单独预测(Z=2.24、P=0.025，Z=2.17、P=0.030)。结论卵巢浆液性癌组织及血清 TBL1XR1、睾素 2均为高表达，与 FIGO分期、淋巴结转移、组织学分级及不良预后密切相关，血清 TBL1XR1联合睾素 2具有较高的预后预测价值。 |
| 英文摘要: |
| Objective To investigate the expression levels of transducin β-like 1X-related protein 1 (TBL1XR1) and testosterone 2 in ovarian serous tumor tissues and their relationship with prognosis.Methods A total of 102 patients with ovarian serous tumor (malig-nant group), 50 patients with ovarian borderline serous tumors (borderline group), and 48 patients with ovarian benign serous tumors(benign group) admitted to hospital from April 2015 to April 2017 were selected from the Affiliated Cancer Hospital of Nantong Univer-sity. Immunohistochemistry was used to detect the protein expression of TBL1XR1 and testosterone 2 in tissues. Real-time fluorescence quantitative PCR was performed to detect the serum mRNA expression levels of TBL1XR1 and testosterone 2. Kaplan-Meier analysiswas performed to analyze the associations between different protein expression profiles of TBL1XR1 and testosterone 2 and the survivaltime. Cox regression modeling was performed to analyze the factors influencing the poor prognosis of the patients in the malignantgroup. Receiver operating characteristic (ROC) curves were analyzed to determine the predictive value of the serum TBL1XR1 and tes-tosterone 2 mRNA for poor prognosis in the malignant group.Results The rates of high TBL1XR1 and testosterone 2 protein expres-sion in the malignant group were greater than those in the borderline group, and the rates in the borderline group were greater thanthose in the benign group (P<0.05). The mRNA expression levels of serum TBL1XR1 (2.27±0.58, 1.41±0.37, 1.06±0.13) and testoster-one 2 (2.66±0.67, 1.58±0.40, 1.09±0.15) in the malignant group, borderline group, and benign group decreased successively (P<0.05). High-grade patients with FIGO stage Ⅲ-Ⅳ disease, lymph node metastasis and histological grade had higher expression levels of theTBL1XR1 and testosterone 2 proteins than patients with FIGO stage Ⅰ-Ⅱ disease, no lymph node metastasis and low-grade disease (P < 0.05). Survival analysis revealed that the 5-year cumulative survival rate was lower in the TBL1XR1 and testosterone 2 high-ex- pression groups than in the TBL1XR1 and testosterone 2 low-expression groups (P<0.05). Cox multivariate regression analysis revealed that FIGO stage Ⅲ -Ⅳ , lymph node metastasis, high-grade histology, high TBL1XR1 expression and high testosterone 2 expressionwere risk factors for poor prognosis in patients with ovarian serous carcinoma (P<0.05). The mRNA expression levels of serum TBL1XR1 (2.65±0.61 vs. 1.82±0.54) and testosterone 2 (3.10±0.76 vs. 2.15±0.57) in patients who died were greater than those in pa- tients who survived in the malignant group (P<0.05). The area under the curve (ROC) of serum TBL1XR1 and testosterone 2 mRNA forpredicting poor prognosis in patients in the malignant group was 0.84; the AUC of the combination of TBL1XR1 and testosterone 2mRNA for predicting poor prognosis was 0.94, which was better than that of TBL1XR1 or testosterone 2 mRNA alone (Z=2.24, P= 0.025; Z=2.17, P=0.030).Conclusions Both tissue and serum TBL1XR1 and testosterone 2 were highly expressed in ovarian seroustumors and were closely related to FIGO stage, lymph node metastasis, histological grade and poor prognosis. The combination of serumTBL1XR1 and testosterone 2 had a high prognostic predictive value. |
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