| 袁军,常时新,王乐,等.双模态碳量子点间充质干细胞成像及细胞分化研究[J].安徽医药,2024,28(10):2094-2098. |
| 双模态碳量子点间充质干细胞成像及细胞分化研究 |
| Research on bimodal carbon dots imaging of mesenchymal stem cells and cell differentiation |
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| DOI：10.3969/j.issn.1009-6469.2024.10.038 |
| 中文关键词: 示踪物 柠檬酸 二乙烯三胺 显微镜检查，共焦 细胞光度测定法 碳量子点 间充质干细胞 双模态成像 成骨分化 成脂肪分化 探针 |
| 英文关键词: Tracer Citric acid Diethylenetriamine Microscopy, confocal Cytophotometry Carbon dots Mesenchymal stem cells Bimodal imaging Osteogenic differentiation Adipogenic differentiation Probe |
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| 摘要点击次数: 821 |
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| 中文摘要: |
| 目的制备双模态碳量子点( carbon dots，CDs)探针并探索其示踪间充质干细胞( MSCs)的可行性。方法 2022年 10月至 2023年 5月，以钆喷酸葡胺、柠檬酸及二乙烯三胺为前驱体，采用水热法合成 Gd∶CDs［钆( Gd)掺杂的碳量子点］，进行表征并用于 MSCs标记及成像。检测不同浓度 Gd∶CDs(10、25、50 nmol/L)对 MSCs成骨分化及成脂肪分化的影响。结果 Gd∶CDs生物安全性好，荧光量子产率为 76%，纵向弛豫率为 5.572 7 mmol-1·s-1，具备双模态成像潜力，可用于 MSCs标记。不同浓度 Gd ∶CDs(10、25、50 nmol/L)标记的 MSCs经成骨诱导后，吸光度［D(λ)］分别为 2.368±0.014、2.253±0.084及 2.133±0.127，与对照组 D(λ)(2.334±0.062)相比，高浓度 Gd∶CDs对 MSCs成骨分化有少许影响( P<0.05)中低浓度的 Gd∶CDs标记对 MSCs成骨分化无明显影响( P>0.05)。不同浓度 Gd∶CDs(10、25、50 nmol/L)标记的 MSCs经成脂肪，诱导后， D(λ)分别为 0.274±0.036、0.286±0.032、0.262±0.065，与对照组 D(λ)(0.254±0.026)相比，各组对 MSCs的成脂肪分化无明显影响( P>0.05)。结论采用水热法制备的 Gd∶CDs荧光量子产率及纵向弛豫率高，具有示踪 MSCs的潜能，且对 MSCs的分化影响较小。 |
| 英文摘要: |
| Objective To prepare bimodal carbon dot probes and explore their feasibility for tracking mesenchymal stem cells (MSCs).Methods Gadolinium-doped carbon dots (Gd∶CDs) were synthesized using Gd-DTPA, citric acid, and diethylenetriamine asprecursors via a hydrothermal method from October 2022 to May 2023. The synthesized Gd∶CDs were characterized and used for labeling and tracing MSCs. The effects of different concentrations of Gd∶CDs (10, 25, 50 nmol/L) on the osteogenic and adipogenic differentiation of MSCs were assessed.Results Gd∶CDs showed good biocompatibility, with a fluorescence quantum yield of 76% and a longitudinal relaxivity of 5.5727 mmol-1·s-1, indicating their potential for bimodal imaging and MSCs labeling. After osteogenic induction of MSCs labeled with different concentrations of Gd∶ CDs (10, 25, 50 nmol/L), the optical density values were 2.368±0.014,2.253±0.084, and 2.133±0.127, respectively. Compared with the control group (optical density value of 2.334±0.062), the high concentration of Gd∶CDs had a slight effect on the osteogenic differentiation of MSCs (P < 0.05), while the medium and low concentration groups showed no significant effect on osteogenic differentiation of MSCs (P > 0.05). After adipogenic induction of MSCs labeled with different concentrations of Gd∶CDs (10, 25, 50 nmol/L),the optical density values were 0.274±0.036, 0.286±0.032, and 0.262±0.065,respectively. Compared with the control group (optical density value of 0.254±0.026), none of the experimental groups showed a significant effect on the adipogenic differentiation of MSCs (P > 0.05).Conclusion The Gd∶CDs synthesized in this experiment exhibitshigh fluorescence quantum yield and longitudinal relaxivity, making them suitable for tracking MSCs with minimal influence on theirdifferentiation. |
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