| 林汉杰,余青,郑钟立,等.外周血中 γ干扰素和白细胞介素 -23表达与复发性流产的相关性研究[J].安徽医药,2024,28(12):2474-2478. |
| 外周血中 γ干扰素和白细胞介素 -23表达与复发性流产的相关性研究 |
| Study on the correlation between the expression of interferon gamma and interleukin-23 in peripheral blood and recurrent miscarriage |
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| DOI：10.3969/j.issn.1009-6469.2024.12.028 |
| 中文关键词: 流产，习惯性 γ干扰素 白细胞介素 -23 免疫功能指标 相关性 诊断 |
| 英文关键词: Abortion, habitual Interferon-γ Interleukin-23 Immune function index Correlation Diagnosis |
| 基金项目:佛山市卫生健康局医学科研课题( 20230221) |
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| 中文摘要: |
| 目的探讨外周血中 γ干扰素( IFN-γ)和白细胞介素 -23(IL-23)表达与复发性流产( RPL)的相关性研究。方法选取 2022年 1—12月佛山市妇幼保健院接收的 50例 RPL病人为研究对象，同期健康体检者 50例为对照组。采用酶联免疫吸附测定( ELISA)检测血清 IFN-γ、IL-23水平；采用多因素 logistic回归分析探索 RPL发生的影响因素；采用 Pearson法分析血清 IFN-γ、IL-23水平与临床资料的相关性；采用受试者操作特征曲线( ROC曲线)评价血清 IFN-γ、IL-23及其联合对 RPL发生的预测效能。结果相较于对照组， RPL组年龄、血红蛋白、 CD4+/CD8+蛋白 C、抗心磷脂抗体( ACA)IgA、IFN-γ［( 25.95±7.44)ng/L比(17.72±4.57)ng/L］、 IL-23［( 4.32±1.37)μg/L比( 2.99±0.81)μg/L］平显著升高( P<0.05)， CD8+、ACA IgG水平显著降低( P<水、0.05)。 logistic分析发现，年龄、 CD4+/CD8+、IFN-γ、IL-23均是 RPL发生的独立危险因素( P<0.05)； CD8+是 RPL发生的独立保护因素( P<0.05)。 RPL病人血清 IFN-γ与 IL-23、年龄、 CD4+/CD8+呈正相关( r=0.61、0.59、0.52，P<0.05)，与 CD8+呈负相关( r= .0.57，P<0.05)。血清 IL-23与年龄、 CD4+/CD8+正相关( r=0.55、0.56，P<0.05)与 CD8+负相关( r=.0.52，P<0.05)。 ROC结果显示血清中 IFN-γ、IL-23水平及二者联合预测 RPL发生的 AUC分别为 0.79、0.77、0，.88，其中联合预测 AUC显著高于二者单独预测 AUC(P<0.05)。结论 RPL病人血清中 IFN-γ、IL-23水平升高，与机体的免疫功能关系密切，均有望成为 RPL发生的预测因子。 |
| 英文摘要: |
| Objective To study the correlation between the expression of interferon gamma (IFN -γ) and interleukin-23 (IL-23) in pe-ripheral blood and recurrent miscarriage (RPL).Methods From January 2022 to December 2022, 50 patients with RPL admitted toFoshan Maternal and Child Health Hospital were selected as research subjects, and 50 healthy subjects during the same period were se-lected as the control group. The levels of serum IFN-γ and IL-23 were detected by enzyme-linked immunosorbent assay (ELISA); Logis-tic multiple factor regression was applied to analyze the influencing factors of RPL; Pearson method was applied to analyze the level ofserum IFN-γ and IL-23 and their correlation with clinical data; the predictive effect of serum IFN-γ, IL-23 and their combination on the occurrence of RPL was evaluated by the ROC curve. Results Compared with the control group, the levels of age, hemoglobin, CD4+/CD8+, protein C, ACA IgA, IFN-γ and IL-23 in RPL group were obviously higher (P<0.05), the levels of CD8+ and ACA IgG were obviously lower (P<0.05). Logistic analysis showed that age, CD4+/CD8+, IFN-γ and IL-23 were independent risk factors for RPL (P< 0.05); CD8+ was an independent protective factor for RPL (P<0.05). Serum IFN-γ in RPL patients was positively correlated with IL-23, age, CD4+/CD8+ (r=0.61, 0.59, 0.52, P<0.05), and negatively correlated with CD8+ (r=.0.57, P<0.05). Serum IL-23 was positively corre-lated with age, CD4+/CD8+ (r=0.55, 0.56, P<0.05), and negatively correlated with CD8+ (r=.0.52, P<0.05). The ROC results showed that the AUC of the levels of IFN-γ and IL-23 in serum and the combination for predicting the occurrence of RPL was 0.79, 0.77 and0.88, respectively, the AUC of combined prediction was obviously higher than the AUC of two independent prediction (P<0.05).Conclu. sion The levels of IFN-γ and IL-23 in the serum of patients with RPL are increased, and are closely related to the immune function ofthe body, which are expected to be the predictors of RPL. |
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