| 王磊,张洁,刘春玲,等.三阴性乳腺癌中 KIAA1522表达和作用研究[J].安徽医药,2025,29(1):44-48. |
| 三阴性乳腺癌中 KIAA1522表达和作用研究 |
| KIAA1522 promotes triple negative breast cancer metastasis by activating the Wnt signaling pathway |
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| DOI：10.3969/j.issn.1009-6469.2025.01.008 |
| 中文关键词: 三阴性乳腺癌 KIAA1522 Wnt信号通路 含 IQ摸序的 GTP酶活化蛋白 1 β连环素 免疫共沉淀 |
| 英文关键词: Triple negative breast neoplasms Wnt signaling pathway KIAA1522 IQ motif containing GTPase activating protein 1 β-catenin Co-Immunocoprecipitation |
| 基金项目:河北省医学科学研究重点课题项目( 20171270) |
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| 中文摘要: |
| 目的分析三阴乳腺癌( TNBC)中 KIAA1522激活对 Wnt信号通路及促进肿瘤细胞转移的机制影响。方法该研究于 2021年 1月至 2022年 10月进行，收集唐山市人民医院手术治疗的三阴乳腺癌 36例， TNBC癌组织依据有淋巴结转移(转移组)和无淋巴结转移(未转移组)分为两组，采用蛋白质印迹法和实时荧光定量逆转录 PCR(RT-qPCR)法分别检测各组 KIAA1522、含 IQ模序的 GTP酶活化蛋白 1(IQGAP1)、 β连环素( β-catenin)的蛋白及 mRNA相对表达水平，免疫共沉淀法检测 KIAA1522、 IQGAP1分别与 β-catenin的相互作用情况。结果转移组中 KIAA1522、IQGAP1、β-catenin蛋白相对表达量( 0.37±0.05、0.28±0.02、1.50±0.08)均高于未转移组( 0.27±0.05、0.25±0.05、1.05±0.02)(P<0.05)。转移组中 KIAA1522、IQGAP-1 mRNA相对表达量( 0.95±0.03、1.08±0.10)高于未转移组( 0.73±0.05、0.99±0.12)(P<0.05)两者呈正相关关系( r=0.55，P<0.05)β-catenin mRNA在二组中的相对表达量差异无统计学意义。免疫共沉淀显示在TNBC癌组，织中 KIAA1522蛋白与 β-catenin蛋白，无相互作用，而 IQGAP1蛋白与 β-catenin蛋白相互共沉淀。结论 KIAA1522激活 Wnt信号通路，促进 TNBC肿瘤细胞的转移，机制可能与上调 IQGAP1 mRNA，过表达的 IQGAP1促进 β-catenin蛋白累积并结合成蛋白复合物有关。 |
| 英文摘要: |
| Objective To analyze the mechanism of KIAA1522 activating Wnt signaling pathway and promoting tumor cell metasta-sis in triple negative breast cancer (TNBC). Methods The study was conducted from January 2021 to October 2022, 36 cases of triplenegative breast cancer treated surgically at Tangshan People's Hospital were collected. TNBC was assigned into two groups accordingwith lymph node metastasis (metastatic group) and without lymph node metastasis (non-metastatic group). The relative expression levels of KIAA1522, IQGAP1 and β-catenin proteins and mRNA in each group were detected by Western blotting and RT-qPCR. The interac-tion of β-catenin respectively with KIAA1522 and IQGAP1 were detected by Co-Immunoprecipitation. Results The relative expres-sion levels of KIAA1522 protein, IQGAP1 protein and β -catenin protein in the metastatic group (0.37±0.05, 0.28±0.02, 1.50±0.08) were significantly higher than those in non-metastatic group (0.27±0.05, 0.25±0.05, 1.05±0.02) (P<0.05). The relative expressions ofKIAA1522 mRNA and IQGAP1 mRNA in the metastatic group (0.95±0.03, 1.08±0.10) were significantly higher than those in non-met-astatic group (0.73±0.05, 0.99±0.12) (P<0.05) and they were positively correlated (r=0.55, P<0.05), the relative expression of β-catenin mRNA in the two groups was not statistically significant. Co-immunocoprecipitation showed no interaction between KIAA1522 protein and β-catenin protein in TNBC, in contrast, IQGAP1 protein co-precipitated with β-catenin protein. Conclusion KIAA1522 activates Wnt signaling pathway and promotes the metastasis of TNBC, the mechanism may be related to upregulating of IQGAP1 mRNA, andthen overexpression IQGAP1 promotes the accumulation of β-catenin proteins and the binding of protein complexes. |
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