| 吕璐,翟春霞,张莹莹.卵巢癌组织 CLDN10、三结构域蛋白 59表达及其临床意义[J].安徽医药,2025,29(1):53-57. |
| 卵巢癌组织 CLDN10、三结构域蛋白 59表达及其临床意义 |
| Expression of CLDN10 and tri-structural domain protein 59 in ovarian cancer tissues and its clinical significance |
| |
| DOI：10.3969/j.issn.1009-6469.2025.01.010 |
| 中文关键词: 卵巢肿瘤 紧密连接蛋白 10 三结构域蛋白 59 病理特征 预后 |
| 英文关键词: Ovarian neoplasms Claudin 10 Tripartite-motif protein 59 Pathological characteristics Prognosis |
| 基金项目:河南省医学科技攻关计划项目( 2018020289) |
|
| 摘要点击次数: 471 |
| 全文下载次数: 231 |
| 中文摘要: |
| 目的分析卵巢癌组织紧密连接蛋白 10(CLDN10)、三结构域蛋白 59(TRIM59)表达及其临床意义。方法该研究选取 2017年 2月至 2019年 2月在鹤壁市人民医院接受手术的 112例卵巢癌病人术中切除的卵巢癌组织及癌旁组织。采用实时荧光定量 PCR法检测 CLDN10、TRIM59 mRNA表达；采用免疫组织化学法检测 CLDN10、TRIM59蛋白表达；采用 Pearson法分析 CLDN10、TRIM59的相关性； CLDN10、TRIM59与预后的关系采用 Kaplan-Meier生存曲线分析；卵巢癌病人预后的危险因素采用 Cox回归分析。结果与癌旁组织相比，卵巢癌组织中 CLDN10 mRNA表达水平( 0.62±0.14比 1.00±0.23)及蛋白阳性表达率( 39.29%比 63.39%)明显降低( P<0.05)TRIM59 mRNA表达水平( 1.64±0.32比 1.01±0.25)及蛋白阳性表达率( 64.29%比 38.39%)明显升高( P<0.05)。根据 Pearson关性分析得知，卵巢癌组织中 CLDN10、TRIM59 mRNA表达水平呈负相关( P<0.05)。卵巢癌组织中 CLDN10、TRIM59表达与国际妇产科联盟( FIGO分期)、分化程度、淋巴结转移有关( P<0.05)。根据 Kaplan-Meier法得知，卵巢癌组织中 CLDN10阳性表达病人 3年生存率高于阴性表达病人( P<0.05)；卵巢癌组织中 TRIM59阳性表达病人 3年阳性生存率低于阴性表达病人( P<0.05)。根据 Cox回归分析表明， CLDN10阴性、 TRIM59阳性是影响卵巢癌病人预后的危险因素［ HR=2.15，95%CI:(1.15，4.00)； HR=3.55，95%CI:(1.55，8.10)］。结论卵巢癌组织中 CLDN10阴性、 TRIM59阳性是卵巢癌预后的危险因素，二者有可能作为有价值的预后标志物。 |
| 英文摘要: |
| Objective To analyze the expression and clinical significance of claudin 10 (CLDN10) and triple structural domain pro-tein 59 (TRIM59) expression in ovarian cancer tissues.Methods In this study, intraoperative resection of ovarian cancer tissues andadjacent tissues were selected from 112 ovarian cancer patients who underwent surgery in Hebi People's Hospital from February 2017to February 2019. Real-time fluorescence quantitative PCR was used to detect CLDN10 and TRIM59 mRNA expression; immunohisto-chemistry was used to detect CLDN10 and TRIM59 protein expression; Pearson method was used to analyse the correlation betweenCLDN10 and TRIM59; the relationship between CLDN10 and TRIM59 and prognosis was used for Kaplan-Meier survival curve analy-sis; risk factors for prognosis of ovarian cancer patients were analysed by Cox regression.Results Compared with the paracancerous tissues, the CLDN10 mRNA expression level (0.62±0.14 vs. 1.00±0.23) and protein-positive expression rate (39.29% vs. 63.39%) were significantly lower in ovarian cancer tissues (P<0.05), and the TRIM59 mRNA expression level (1.64±0.32 vs. 1.01±0.25) and protein-positive expression rate (64.29% vs. 38.39%) were significantly higher (P < 0.05). According to the Pearson correlation analysis, the ex-pression levels of CLDN10 and TRIM59 mRNA in ovarian cancer tissues were negatively correlated (P < 0.05). The expression ofCLDN10 and TRIM59 in ovarian cancer tissues was correlated with the International Federation of Gynecology and Obstetrics (FIGOstaging), the degree of differentiation, and lymph node metastasis (P < 0.05). According to the Kaplan-Meier method, it was known that the 3-year survival rate of patients with positive CLDN10 expression in ovarian cancer tissues was higher than that of patients with neg-ative expression (P < 0.05); the 3-year positive survival rate of patients with positive TRIM59 expression in ovarian cancer tissues waslower than that of patients with negative expression (P < 0.05). According to Cox regression analysis, CLDN10-negative and TRIM59-positive were risk factors affecting the prognosis of ovarian cancer patient [HR=2.15, 95%CI: (1.15, 4.00); HR=3.55, 95%CI: (1.55, 8.10)].Conclusion CLDN10-negative and TRIM59-positive ovarian cancer tissues are prognostic factors in ovarian cancer, and bothhave the potential to serve as valuable prognostic markers. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
