| 孙颖,顾玮,王吉,等.lncRNA-BBOX1-2通过调控成纤维细胞生长因子受体 1促进胃癌的发生和发展[J].安徽医药,2025,29(1):57-63. |
| lncRNA-BBOX1-2通过调控成纤维细胞生长因子受体 1促进胃癌的发生和发展 |
| LncRNA-BBOX1-2 promotes the occurrence and progression of gastric cancer by regulating FGFR1 |
| |
| DOI：10.3969/j.issn.1009-6469.2025.01.011 |
| 中文关键词: 胃肿瘤 长链非编码 RNA BBOX1-2 成纤维细胞生长因子受体 1 调控 增殖 凋亡 |
| 英文关键词: Stomach neoplasms LncRNA-BBOX1-2 Fibroblast growth factor receptor 1 Trans-regulation Proliferation Apoptosis |
| 基金项目:上海市卫计委科研项目( 201540572) |
|
| 摘要点击次数: 518 |
| 全文下载次数: 164 |
| 中文摘要: |
| 目的探讨长链非编码 RNA(long non-coding RNA，lncRNA)BBOX1-2通过调控成纤维细胞生长因子受体 1(fibroblast growth factor receptor 1，FGFR1)对胃癌的发生发展机制的影响。方法回顾性选取 2017年 4月至 2019年 1月于上海交通大学医学院附属瑞金医院卢湾分院接受胃癌根治术 30例病人肿瘤组织及癌旁相应正常组织作为研究对象，采用实时定量 PCT(re.al-time PCR，RT-PCR)检测 lncRNA-BBOX1-2和 FGFR1表达； si-linc-BBOX1-2转染 SGC-7901细胞后，通过蛋白质印迹法 /细胞存活率分析( MTT)、细胞迁移和侵袭( Transwell)实验、细胞划痕、平板克隆一系列生物学功能实验，检测肿瘤细胞生物学功能及 FGFR1表达的变化。结果胃癌组织中的 lncRNA-BBOX1-2(3.68±0.58比 1.15±0.11)和 FGFR1(4.26±0.71比 1.19±0.18)表达显著高于癌旁正常组织( P<0.05)； si-linc-BBOX1-2转染 SGC-7901细胞后， FGFR1表达下调，细胞活力、迁移、侵袭和生存能力明显下降。结论 LincRNA-BBOX1-2可通过调控 FGFR1的表达介导胃癌细胞的增殖、凋亡、迁移和侵袭，可能为胃癌的治疗提供了新的靶点和潜在的生物学标志物。 |
| 英文摘要: |
| Objective To investigate the effect of long non-coding RNA (lncRNA) BBOX 1-2 on the development mechanism of gas-tric cancer (GC) by regulating fibroblast growth factor receptor 1 (FGFR1).Methods Tumor tissues and corresponding normal tissuesadjacent GC of 30 patients who underwent radical gastrectomy in Luwan Branch of Ruijin Hospital of Shanghai Jiao Tong UniversitySchool of Medicine from 4, 2017 to 1, 2019 were retrospectively selected as the study objects, and real-time PCR was used to detect the expression of lncRNA-BBOX1-2 and FGFR1. After SGC-7901 cells transfected with si-linc-BBOX1-2, the changes in tumor cell biolog-ical function and FGFR1 expression by a series of biological function experiments of western blotting, MTT, Transwell, cell scratches,and plate cloning were detected.Results Both lncRNA-BBOX1-2 (3.68±0.58 vs. 1.15±0.11) and FGFR1 (4.26±0.71 vs. 1.19±0.18) were overexpressed in GC tissues than those in adjacent normal tissues (P<0.05). si-linc-BBOX 1-2 transfection of SGC-7901 cells showed downregulation of FGFR1 expression and significantly decreased cell viability, migration, invasion and viability.Conclusion LincRNA-BBOX 1-2 can mediate the proliferation, apoptosis, migration and invasion of GC cells by regulating FGFR1 expression,which may provide new targets and potential biological markers for the treatment of GC. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
