| 唐建军,黄晓蓉,吴逢斌,等.膝骨关节炎病人血清 ADAMTS5和 CXCR4表达与病情严重程度的相关性分析[J].安徽医药,2025,29(1):95-99. |
| 膝骨关节炎病人血清 ADAMTS5和 CXCR4表达与病情严重程度的相关性分析 |
| Correlation analysis between the expression of ADAMTS5 and CXCR4 in serum and the severity of knee osteoarthritis |
| |
| DOI：10.3969/j.issn.1009-6469.2025.01.019 |
| 中文关键词: 骨关节炎，膝 解聚蛋白样金属蛋白酶 5 趋化因子受体 4 炎症因子 病情严重程度 |
| 英文关键词: Osteoarthritis, knee Disintegrin-like and metalloproteinase with thrombospondin type l motif 5 Chemokine receptor 4 Inflammatory factors Severity |
| 基金项目:四川省中医药管理局科学技术研究项目( 2020LC0054) |
|
| 摘要点击次数: 538 |
| 全文下载次数: 231 |
| 中文摘要: |
| 目的探究膝骨关节炎( KOA)病人血清解聚蛋白样金属蛋白酶 5(ADAMTS5)和趋化因子受体 4(CXCR4)表达水平与病情严重程度的相关性。方法选取 2021年 1月至 2023年 1月在成都医学院第一附属中医医院就诊治疗的 KOA病人 136例为研究对象，根据 KL分级将 KOA病人分为轻度组 46例、中度组 68例、重度组 22例；另选取同期在该院体检健康者 136例作为健康组。采用酶联免疫吸附测定(ELISA)检测血清 ADAMTS5、CXCR4和白细胞介素 -17(IL-17)、肿瘤坏死因子 α(TNF-α)、基质金属蛋白酶组织抑制因子 -1(TIMP-1)水平；采用 Pearson分析血清 ADAMTS5和 CXCR4水平与相关指标相关性；采用受试者操作特征曲线( ROC曲线)分析血清 ADAMTS5和 CXCR4在 KOA重度病人中的诊断价值。结果与健康组相比较， KOA组的炎症因子 IL-17、TNF-α水平和身体质量指数均显著上调， TIMP-1水平显著下调( P<0.05)； KOA组血清 ADAMTS5(3.63±1.15)g/L水平较健康组( 2.23±0.59)g/L显著上调( P<0.05)； KOA组血清 CXCR4(302.48±53.46)ng/L水平较健康组( 163.97±35.82)ng/L显著上调(P<0.05)； KOA病人血清 ADAMTS5和 CXCR4水平随 KOA病情严重程度的加重均呈现逐渐上升的趋势(P<0.05)； Pearson 相关分析显示， KOA病人血清 ADAMTS5、CXCR4水平与 IL-17、TNF-α、身体质量指数均呈正相关，与 TIMP-1呈负相关( P<0.05)； ROC结果显示，血清 ADAMTS5、CXCR4诊断 KOA重度病人的曲线下面积(AUC)分别为 0.89、0.89，两者联合诊断的 AUC为 0.98，均优于其各自单独诊断(Z=2.25、3.06，P<0.05)，且特异度为 96.49%，灵敏度为 95.45%。结论 KOA病人血清 ADAMTS5、CXCR4水平均显著上调，两者表达水平随病人病情严重程度的加重而逐渐上升，且在诊断重症 KOA病人中具有重要价值。 |
| 英文摘要: |
| Objective To investigate the correlation between the expression levels of serum depolymerin-like metalloproteinase 5(ADAMTS5) and chemokine receptor 4 (CXCR4) in patients with knee osteoarthritis (KOA) and the severity of the disease.Methods A total of 136 KOA patients who were treated in the First Affiliated Hospital of Traditional Chinese Medicine of Chengdu Medical Col-lege from January 2021 to January 2023 were regarded as the study subjects, according to Kellgren Lawrence (KL) classification, KOApatients were grouped into mild group (46 cases), moderate group (68 cases) and severe group (22 cases); in addition, 136 healthy peo-ple in the same period in our hospital were regarded as the healthy control group. Serum levels of ADAMTS5, CXCR4, interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α) and tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) were measured by enzyme-linked immunosorbent assay (ELISA); Pearson was applied to analyze the correlation between serum ADAMTS5 and CXCR4 levels and relat-ed indicators; the diagnostic value of serum ADAMTS5 and CXCR4 in patients with severe KOA was analyzed by the ROC curve.Re. sults Compared with the healthy control group, the levels of inflammatory factors IL-17, TNF-α and body mass index in KOA group were obviously increased, while the level of TIMP-1 was obviously decreased (P<0.05). The serum levels of ADAMTS5 (3.63±1.15)g/Lin the KOA group were significantly elevated compared to the healthy control group (2.23±0.59)g/L. The serum levels of CXCR4(302.48±53.46)ng/L in the KOA group were significantly elevated compared to the healthy control group (163.97±35.82)ng/L (P<0.05);the levels of serum ADAMTS5 and CXCR4 in patients with KOA showed a gradual increase with the aggravation of the severity of KOA(P<0.05); Pearson correlation analysis showed that serum ADAMTS5 and CXCR4 levels in KOA patients were positively correlatedwith IL-17, TNF-α and body mass index, and negatively correlated with TIMP-1(P<0.05); ROC curve results showed that the area un-der the curve (AUC) of serum ADAMTS5 and CXCR4 in the diagnosis of severe KOA patients was 0.89 and 0.89, respectively, theAUC of the combined diagnosis of the two was 0.98, which was better than their respective diagnosis alone (Z=2.25 and 3.06, P<0.05), with specificity of 96.49% and sensitivity of 95.45%.Conclusion The levels of serum ADAMTS5 and CXCR4 in KOA patients are ob-viously increased, the expression levels of both of them gradually increase with the aggravation of the severity of the patient's condition,and have important value in the diagnosis of severe KOA patients. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
