| 顾玉,梁晓艳,马胜辉,等.肾病综合征病人血清 lncRNA ANRIL和 BDNF水平变化与血栓栓塞的相关性分析[J].安徽医药,2025,29(1):141-146. |
| 肾病综合征病人血清 lncRNA ANRIL和 BDNF水平变化与血栓栓塞的相关性分析 |
| Correlation analysis between changes of serum lncRNA ANRIL and BDNF levels and thromboembolism in patients with nephrotic syndrome |
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| DOI：10.3969/j.issn.1009-6469.2025.01.029 |
| 中文关键词: 肾病综合征 非编码 RNA ANRIL 脑源性神经营养因子 血栓栓塞 预测价值 |
| 英文关键词: Nephrotic syndrome Non-coding RNA ANRIL Brain derived neurotrophic factor Thromboembolism Predictive value |
| 基金项目:承德市科学技术研究课题计划项目( 202002A017) |
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| 中文摘要: |
| 目的探究肾病综合征( NS)病人血清长链非编码 RNA(lncRNA)细胞周期激酶抑制因子 4基因座中反义非编码 RNA(ANRIL)和脑源性神经营养因子( BDNF)水平变化与血栓栓塞( TE)的相关性。方法选取 2019年 3月至 2021年 12月在承德市中心医院住院治疗的 220例 NS病人为研究对象，根据随访结果将其分为 NS组 118例和 TE组 102例，另外选取同期体检健康者 100例为对照组。采用实时荧光定量 PCR(qRT-PCR)测定研究对象血清 lncRNA ANRIL水平；采用酶联免疫吸附测定(ELISA)检测研究对象血清 BDNF水平；使用全自动生化分析仪检测相关生化指标；采用 Pearson相关性分析血清 lncRNA ANRIL、 BDNF水平与生化指标相关性；采用多因素 logistic回归分析 NS病人发生 TE的影响因素；采用受试者操作特征曲线( ROC曲 线)分析血清 lncRNA ANRIL、BDNF在预测 NS病人发生 TE中的价值。结果与对照组相比较， NS组、 TE组血清 lncRNA ANRIL水平显著升高(2.54±0.43比 5.82±1.34、8.35±2.17)，BDNF水平显著降低［(32.77±8.25)μg/L比(24.49±4.58)μg/L、(18.63±3.62)μg/L］(P<0.05)；与 NS组相比较， TE组血清 lncRNA ANRIL水平显著升高， BDNF水平显著降低(P<0.05)；对照组、 NS组、 TE组三组的尿酸、血肌酐、乳酸脱氢酶、血尿素氮、尿蛋白水平呈逐渐升高趋势，血清白蛋白、血红蛋白水平呈逐渐降低趋势(P<0.05)。Pear-son相关性分析显示， NS合并 TE病人血清 lncRNA ANRIL水平与尿酸、血肌酐、乳酸脱氢酶、血尿素氮、尿蛋白水平呈正相关，与血清白蛋白、血红蛋白水平呈负相关(P<0.05)； NS合并 TE病人血清 BDNF水平与尿酸、血肌酐、乳酸脱氢酶、血尿素氮、尿蛋白水平呈负相关，与血清白蛋白、血红蛋白水平呈正相关(P<0.05)。多因素 logistic回归分析显示， lncRNA ANRIL、尿蛋白和 BDNF是 NS病人发生 TE的影响因素(P<0.05)； ROC曲线结果显示，血清 lncRNA ANRIL、BDNF预测 NS病人发生 TE的曲线下面积(AUC)分别为 0.84、0.85，两者联合预测的 AUC为 0.92，高于 lncRNA ANRIL、BDNF单独检测，特异度为 83.05%，灵敏度为 88.24%。结论 NS伴 TE病人中血清 lncRNA ANRIL水平升高， BDNF水平降低，二者对 NS病人发生 TE具有一定的预测价值。 |
| 英文摘要: |
| Objective To investigate the correlation between the changes of long non-coding RNA (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) and brain-derived neurotrophic factor (BDNF) levels and thromboembolism (TE) in patients with ne-phrotic syndrome (NS). Methods Two hundred and twenty NS patients who were hospitalized in Chengde Central Hospital fromMarch 2019 to October 2021 were selected as the study subjects, including 102 NS patients with TE as the TE group, 118 NS patientswithout TE as the NS group, and 100 healthy persons in the same period were regarded as the control group. Real-time fluorescence quantitative PCR (qRT-PCR) was applied to measure the serum lncRNA ANRIL level of the subjects; the serum BDNF level of the sub-jects was detected by enzyme-linked immunosorbent assay (ELISA); automatic biochemical analyzer was applied to detect relevant bio-chemical indicators; Pearson correlation was applied to analyze the correlation between serum lncRNA ANRIL, BDNF levels and bio-chemical indicators; multivariate Logistic regression was applied to analyze the influencing factors of TE in NS patients; the predictivevalue of serum lncRNA ANRIL and BDNF on the occurrence of TE in NS patients was analyzed by using the Receiver operating Char-acteristic curve (ROC curve). Results Compared with the control group, the serum lncRNA ANRIL level in NS group and TE group was significantly increased (2.54±0.43 vs. 5.82±1.34, 8.35±2.17). The level of BDNF was significantly decreased [(32.77±8.25) μg/L vs. (24.49±4.58) μg/L, (18.63±3.62) μg/L] (P < 0.05). Compared with NS group, the serum lncRNA ANRIL level in TE group was sig-nificantly increased, and the serum BDNF level was significantly decreased (P < 0.05). The levels of uric acid, serum creatinine, lactatedehydrogenase, blood urea nitrogen, and urine protein in the control group, NS group and TE group were gradually increased, while thelevels of serum albumin, and hemoglobin were gradually decreased (P<0.05). Pearson correlation analysis showed that the level of se-rum lncRNA ANRIL was positively correlated with the levels of uric acid, serum creatinine, lactate dehydrogenase, blood urea nitrogenand urine protein in NS patients with TE, and negatively correlated with the levels of serum albumin and hemoglobin (P<0.05); the lev-el of serum BDNF in NS patients with TE was negatively correlated with the levels of uric acid, creatinine, lactate dehydrogenase, bloodurea nitrogen and urine protein, and positively correlated with the levels of serum albumin and hemoglobin (P<0.05). Multivariate Logis-tic regression analysis showed that lncRNA ANRIL, urinary protein, and BDNF were influential factors for the occurrence of TE in NSpatients (P<0.05); the ROC curve results showed that the area under the curve (AUC) of serum lncRNA ANRIL and BDNF in predict-ing the occurrence of TE in NS patients was 0.84 and 0.85, respectively, the AUC predicted by the combination of the two was 0.92,which was higher than that of the detection of lncRNA ANRIL and BDNF alone, with specificity of 83.05% and sensitivity of 88.24%.Conclusion The level of serum lncRNA ANRIL is increased and the level of BDNF is decreased in NS patients with TE, both havecertain predictive value for the occurrence of TE in NS patients. |
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