| 齐飞,汪晶,白志峰,等.高良姜素通过调节 miR-124/STAT3轴对动脉粥样硬化大鼠血管内皮细胞损伤的影响[J].安徽医药,2025,29(2):258-263. |
| 高良姜素通过调节 miR-124/STAT3轴对动脉粥样硬化大鼠血管内皮细胞损伤的影响 |
| Effect of galangin on vascular endothelial cell injury in atherosclerotic rats by regulating miR-124/STAT3 axis |
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| DOI：10.3969/j.issn.1009-6469.2025.02.009 |
| 中文关键词: 动脉粥样硬化 高良姜素 微 RNA124 信号转导与转录激活因子 3 血管内皮细胞 |
| 英文关键词: Atherosclerosis Galangin Micro RNA124 Signal transducer and activator of transcription 3 Vascular endothelial cells |
| 基金项目:首都医科大学附属北京天坛医院专项课题( 2016YFC1301501) |
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| 中文摘要: |
| 目的探讨高良姜素通过调节微 RNA124(miR-124)/信号转导与转录激活因子 3(STAT3)轴对动脉粥样硬化( AS)大鼠血管内皮细胞损伤的影响。方法研究时间为 2023年 1―5月，原代培养大鼠胸主动脉内皮细胞分为对照组(未做任何处理)、模型组(80 mg/L ox-LDL处理)高良姜素组( 50 μmol/L高良姜素 +80 mg/L ox-LDL处理)、高良姜素 +inhibitor NC组(50 μmol/L高良姜素 +80 mg/L ox-LDL处理后转、染 inhibitor NC)、高良姜素 +miR-124 inhibitor组( 50 μmol/L高良姜素 +80 mg/L ox-LDL处理后转染 miR-124 inhibitor)。双萤光素酶报告基因实验验证 miR-124、STAT3靶向关系；实时荧光定量逆转录聚合酶链式反应(qRT-PCR)检测 miR-124表达；蛋白质印迹法检测 STAT3蛋白表达； MTT法测定细胞增殖；流式细胞术检测细胞凋亡；酶联免疫吸附测定( ELISA)检测炎症因子以及一氧化氮( NO)、活性氧( ROS)水平。结果 miR-124靶向调控 STAT3表达。与对照组相比，模型组 miR-124水平( 1.00±0.00比 0.45±0.06)、 D(λ)490值( 0.98±0.13比 0.44±0.05)显著下降( P<0.05)，凋亡率［( 5.58±0.68)%比( 15.83±2.06)%］、 STAT3水平( 0.48±0.05比 1.08±0.13)、 IL-1β［( 12.05±1.17)mg/L比( 22.42±2.36)mg/L］、IL-6［( 4.13±0.48)mg/L比( 9.64±0.98)mg/L］、 TNF-α［( 2.11±0.25)mg/L比( 6.63±0.66)mg/L］、 NO［( 22.26±2.33)μmol/L比( 90.25±9.44)μmol/L］、 ROS［(32.93±3.52)μmol/L比( 60.91±7.19)μmol/L］水平显著升高( P<0.05)；与模型组相比，高良姜素组 miR-124水平( 0.45±0.06比 0.89±0.01)、 D(λ) 值( 0.44±0.05比 0.84±0.09)显著升高( P<0.05)，凋亡率［( 15.83±2.06)%比( 6.28±0.72)%］、 STAT3水平(1.08±0.13比0.53±0.06)490、IL-1β［( 22.42±2.36)mg/L比( 16.92±1.93)mg/L］、IL-6［( 9.64±0.98)mg/L比 5.42±0.53)mg/L］、 TNF-α［( 6.63±0.66)mg/L比( 3.57±0.37)mg/L］、 NO［( 90.25±9.44)μmol/L比( 34.84±4.10)μmol/L］、 ROS［( 60.91±7.19)μmol/L比( 30.41±3.05)μmol/L］水平显著下降( P<0.05)，下调 miR-124抑制高良姜素对 AS大鼠血管内皮细胞损伤的改善。结论高良姜素通过上调 miR-124来下调 STAT3表达，从而减轻 AS大鼠血管内皮细胞损伤。 |
| 英文摘要: |
| Objective To investigate the effect of galangin on vascular endothelial cell injury in atherosclerosis (AS) rats by regulat-ing the micro RNA124 (miR-124)/signal transducer and activator of transcription 3 (STAT3) axis.Methods The study started fromJanuary 2023 and ended in May 2023. Primary cultured rat aortic endothelial cells were grouped into control group (without any treat-ment), model group (80 mg/L ox-LDL treatment), galangin group (50 μmol/L galangin+80 mg/L ox-LDL treatment), galangin+inhibitor NC group (transfected with inhibitor NC after treatment with 50 μmol/L galangin+80 mg/L ox-LDL), galangin+miR-124 inhibitor group (transfected with miR-124 inhibitor after treatment with 50 μmol/L galangin+80 mg/L ox-LDL). The dual luciferase gene reporter assay was applied to verify the targeting relationship between miR-124 and STAT3, real-time fluorescent quantitative reverse transcription polymerase chain reaction (qRT-PCR) to detect miR-124 expression, Western blotting to detect STAT3 protein expression, MTT meth-od to measure cell proliferation, flow cytometry to detect cell apoptosis, and enzyme-linked immunosorbent assay (ELISA) to detect thelevels of inflammatory factors, nitric oxide (NO) and reactive oxygen species (ROS). Results MiR-124 targeted and regulated the STAT3 expression. Compared with the control group, the miR-124 level (1.00±0.00 vs. 0.45±0.06) and D(λ)490 value (0.98±0.13 vs. 0.44±0.05) in the model group were obviously decreased (P<0.05), while the apoptosis rate [(5.58±0.68) % vs. (15.83±2.06) %], STAT3 level (0.48±0.05 vs. 1.08±0.13), and the levels of IL-1β [(12.05±1.17) mg/L vs. (22.42±2.36) mg/L], IL-6 [(4.13±0.48) mg/L vs. (9.64± 0.98) mg/L], TNF-α [(2.11±0.25) mg/L vs. (6.63±0.66) mg/L], NO [(22.26±2.33) μmol/L vs. (90.25±9.44) μmol/L], ROS [(32.93±3.52) μmol/L vs. (60.91±7.19) μmol/L] were obviously increased (P<0.05). Compared with the model group, the miR-124 level (0.45±0.06 vs. 0.89±0.01) and D(λ)490 value (0.44±0.05 vs. 0.84±0.09) in the galangin group were obviously increased (P<0.05), while the apoptosis rate [(15.83±2.06) % vs. (6.28±0.72) % ], and the levels of STAT3 (1.08±0.13) vs. (0.53±0.06), IL-1β [(22.42±2.36) mg/L vs. (16.92± 1.93) mg/L[, IL-6 [(9.64±0.98) mg/L vs. (5.42±0.53) mg/L], TNF-α [(6.63±0.66) mg/L vs. (3.57±0.37) mg/L], NO [(90.25±9.44) μmol/L vs. (34.84±4.10) μmol/L], and ROS [(60.91±7.19) μmol/L vs. (30.41±3.05) μmol/L] were obviously decreased (P<0.05). Down-regula-tion of miR-124 inhibited the improvement of galangin on vascular endothelial cell injury in AS rats.Conclusion Galangin can down-regulate the expression of STAT3 by up-regulating miR-124, thereby alleviating the injury of vascular endothelial cells in AS rats. |
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