| 王科程,程德均,张鑫,等.血清 circ核因子 Ⅰ/Ⅹ的表达与慢性心力衰竭心肌重构和心功能的关系[J].安徽医药,2025,29(2):353-357. |
| 血清 circ核因子 Ⅰ/Ⅹ的表达与慢性心力衰竭心肌重构和心功能的关系 |
| Serum circNfix expression in patients with chronic heart failure and its relationship with myocardial remodeling and cardiac function |
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| DOI：10.3969/j.issn.1009-6469.2025.02.029 |
| 中文关键词: 心力衰竭 环状 RNA circ核因子 Ⅰ/Ⅹ 心肌重构 心功能 |
| 英文关键词: Heart failure Circular RNA CircNfix Myocardial remodeling Cardiac function |
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| 中文摘要: |
| 目的分析血清 circ核因子 Ⅰ/Ⅹ(Nfix)的表达与慢性心力衰竭心肌重构和心功能的关系。方法选取 2020年 7月至 2021年 8月于三二〇一医院进行治疗的慢性心力衰竭病人 105例作为观察组，另选取同期在三二〇一医院体检健康者 52例为对照组。采用实时荧光定量 PCR检测血清 circNfix表达水平；采用超声心动图和二维平面图检测心肌重构及心功能相关指标；用全自动电化学发光免疫分析仪检测 N末端 B型利钠肽原( NT-proBNP)水平，放射免疫法测定肿瘤坏死因子 -α(TNF-α)水平；采用 Pearson相关性分析血清 circNfix表达与 NT-proBNP、TNF-α、心肌重构和心功能指标的相关性；采用多因素 logistic回归分析影响慢性心力衰竭发生的危险因素。结果观察组 Ⅰ~Ⅱ级血清 circNfix表达水平显著低于对照组( 0.62±0.14比 1.01±0.23， P<0.05)Ⅲ级 0.49±0.12和Ⅳ级 0.19±0.05表达水平依次显著低于 Ⅰ~Ⅱ级( P<0.05)；观察组 Ⅰ~Ⅱ级 NT-proBNP［( 9.32±2.14) μg/L比(5.7，3±1.11)μg/L］和 TNF-α［(35.27±10.24)ng/L比( 24.94±6.58)ng/L］表达水平高于对照组( P<0.05)，Ⅲ级［(12.90±2.78) μg/L、(54.84±12.29)ng/L］和 Ⅳ级［(15.76±3.06)μg/L、(92.93±16.69)ng/L］表达水平依次显著高于 Ⅰ~Ⅱ级( P<0.05)。观察组 Ⅰ ~Ⅱ级左室舒张末期内径( LVEDD)、左心房内径( LAD)、左心室后壁厚度( LVPWD)和左心室质量指数(LVMI)均显著高于对照组( P<0.05)Ⅳ级显著高于 Ⅲ和Ⅰ~Ⅱ级( P<0.05)而左心室重构指数( LVRI)、心排血量( CO)和左心室射血分数( LVEF)均显著低于对照组(，P<0.05)，Ⅳ级显著低于 Ⅲ和Ⅰ~Ⅱ级(，P<0.05)。根据 pearson相关性分析得知，观察组血清 circNfix表达水平与 NT-proBNP、TNF-α、LVEDD、LVPWD和 LVMI呈负相关( P<0.05)，而与 CO和 LVEF呈正相关( P<0.05)。根据 logistic回归分析得知， circNfix低水平是影响慢性心力衰竭发生的危险因素( P<0.05)。结论慢性心力衰竭病人血清 circNfix表达量降低，并与心肌重构和心功能密切相关。 |
| 英文摘要: |
| Objective To analyze the expression level of circNfix in serum of patients with chronic heart failure and its relationshipwith myocardial remodeling and cardiac function.Methods From July 2020 to August 2021, 105 patients with chronic heart failurewho were treated in 3201 hospital were regarded as the study group, in addition, 52 healthy people were selected as the control group.The expression of circNfix in serum was detected by real-time fluorescent quantitative PCR; echocardiography and two-dimensional pla-nar images were used to detect myocardial remodeling and cardiac function related indicators; the level of NT-proBNP was detected by automatic electrochemiluminescence immunoassay; the level of TNF-α was measured by radioimmunoassay; Pearson correlation wasused to analyze the correlation between the expression of circNfix in serum and NT-proBNP, TNF-α, myocardial remodeling and cardi-ac function; Multivariate logistic regression was used to analyze risk factors affecting the development of chronic heart failure.Results The expression level of circNfix in grade I-Ⅱ in the study group was obviously lower than that in the control group (0.62±0.14 vs. 1.01±0.23, P<0.05), the expression level in grade Ⅲ and grade Ⅳ was obviously lower than that in grade Ⅰ-Ⅱ (0.49±0.12,0.19±0.05 vs. 0.62±0.14, P<0.05); the expression levels of NT-proBNP [(9.32±2.14)μg/L vs. (5.73±1.11)μg/L] and TNF-α [(35.27±10.24)ng/L vs. (24.94±6.58)ng/L] in grade Ⅰ -Ⅱ in the study group were obviously higher than the control group (P<0.05), the expression levels ingrade Ⅲ [(12.90±2.78)μg/L, (54.84±12.29)ng/L] and grade Ⅳ [(15.76±3.06)μg/L, (92.93±16.69)ng/L] were obviously lower than thosein grade Ⅰ-Ⅱ (P<0.05). LVEDD, LAD, LVPWD and LVMI in grade Ⅰ-Ⅱ in the study group were obviously higher than those in the control group (P<0.05), and those in grade Ⅳ were obviously higher than those in grade Ⅲ and grade Ⅰ-Ⅱ (P<0.05), LVRI, CO and LVEF were obviously lower than those in the control group (P<0.05), and those in grade Ⅳ were obviously lower than those in grade Ⅲ and Ⅰ-Ⅱ (P<0.05). According to Pearson correlation analysis, the expression level of circNfix in serum of the study group was nega-tively related to NT-proBNP, TNF-α, LVEDD, LVPWD and LVMI (P<0.05), but positively correlated with CO and LVEF (P<0.05). Ac-cording to logistic regression analysis, low circNfix levels are a risk factor for the development of chronic heart failure (P<0.05).Conclu. sion The expression of circNfix in serum is decreased in patients with chronic heart failure,which is closely related to myocardial re-modeling and cardiac function. |
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