| 李春红,杨洁,黄婧菲,等.hsa_circ_0077223在子宫内膜样癌中表达及意义[J].安徽医药,2025,29(2):379-384. |
| hsa_circ_0077223在子宫内膜样癌中表达及意义 |
| The expression and significance of hsa_circ_0077223 in endometrioid endometrial carcinoma |
| |
| DOI：10.3969/j.issn.1009-6469.2025.02.034 |
| 中文关键词: 癌，子宫内膜样 hsa_circ_0077223 早期诊断 |
| 英文关键词: Carcinoma,endometrioid Hsa_circ_0077223 Early diagnosis |
| 基金项目: |
|
| 摘要点击次数: 602 |
| 全文下载次数: 335 |
| 中文摘要: |
| 目的探讨 hsa_circ_0077223在子宫内膜样癌( EEC)中的表达水平及其与临床病理特征的关系，为 EEC研究高效能分子诊断标志物提供理论依据。方法选取 2例 EEC组织和 2例正常子宫内膜组织进行高通量测序分析，以 P<0.05，且|log2Fold Changes|≥1.5为筛选标准，选择上调显著的 4个 circRNA中 Log2FC最高的 hsa_circ_0077223作为研究对象，后在来自 2021年 7月至 2022年 7月潍坊市人民医院 35例 EEC组织和 35例正常子宫内膜组织中进行验证。生物信息学分析: GO富集分析， hsa_circ_0077223潜在结合位点的反向基因预测， hsa_circ_0077223编码能力预测。结果 EEC中 hsa_circ_0077223表达水平显著高于正常对照组(1.646±1.102比 0.653±0.457)且 hsa_circ_0077223表达水平与 FIGO分期和组织分化程度有关； ROC曲线显示 AUC为 0.85，95%CI:(0.76，0.94)灵敏度为 0.7，7，特异度为 0.89，最大约登指数为 0.66，差异有统计学意义( P<0.001)。 CPAT分析 hsa_circ_0077223具有翻译成多，肽潜能， circbank数据库预测了 49个存在 hsa_circ_0077223结合位点的 miRNA。结论 hsa_circ_0077223在正常组织中表达量显著低于 EEC组织且与 EEC组织分化级别和分期紧密相关， hsa_circ_0077223有望作为新分子标志物成为 EEC早期筛查的高效能指标。 |
| 英文摘要: |
| Objective To investigate the expression level of hsa_circ_0077223 in endometrioid endometrial carcinoma (EEC) and itsrelationship with clinicopathological features, so as to provide theoretical basis for the study of high performance biomarkers in EEC.Methods Two EEC tissues and two normal endometrial tissues were selected for high-throughput sequencing analysis. P<0.05 and log2Fold Changes≥1.5 as the screening criteria, four circRNAs significantly up-regulated were selected for primer design to screen outhsa_circ_0077223, which was verified in 35 EEC tissues and normal endometrial tissues obtained from Weifang People's Hospital fromJuly 2021 to July 2022. Bioinformatics analysis included GO enrichment analysis, downstream gene prediction of hsa_circ_0077223 po-tential binding site, and coding ability prediction of hsa_circ_0077223.Results The expression level of hsa_circ_0077223 in EEC was significantly higher than that in normal control group (1.646±1.102 vs. 0.653±0.457), and the expression level of hsa_circ_0077223was related to FIGO staging and the degree of tissue differentiation. The ROC curve showed that AUC was 0.85, 95%CI (0.76, 0.94), sensitivity 0.77, specificity 0.89, and the maximum approximation index 0.66, with statistically significant differences (P<0.001). CPATanalysis results showed that hsa_circ_0077223 had the potential to be translated into peptides, and circbank database predicted 49miRNAs with binding sites of hsa_circ_0077223.Conclusions The expression level of hsa_circ_0077223 in normal tissues is signifi-cantly lower than that in EEC tissues and is closely related to the degree of differentiation and staging of EEC tissues. Hsa_circ_0077223 is expected to be a new molecular marker and an effective indicator for early screening of EEC. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
