| 姜宏亮,石晓宇.沉默长链非编码RNA C2orf48 对人胃癌细胞AGS恶性行为的影响[J].安徽医药,待发表. |
| 沉默长链非编码RNA C2orf48 对人胃癌细胞AGS恶性行为的影响 |
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| 投稿时间：2025-03-13 录用日期：2025-04-17 |
| DOI： |
| 中文关键词: 长链非编码RNA C2orf48 胃癌 侵袭 真核细胞翻译起始因子4A3 血管内皮生长因子A |
| 英文关键词: |
| 基金项目:2021年巴彦淖尔市医院院内科研项目(2021020) |
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| 中文摘要: |
| 目的 探讨沉默长链非编码RNA C2orf48(LncRNA C2orf48)对人胃癌细胞AGS恶性行为的影响。方法 采用RT-qPCR检测C2orf48在胃癌组织和胃癌细胞系中的表达水平。沉默C2orf48表达AGS细胞为C2orf48组,未进行转染为阴性对照(NC)组,采用CCK-8实验检测AGS细胞增殖；采用流式细胞术检测AGS细胞凋亡和细胞周期；Transwell小室体外侵袭实验检测AGS细胞侵袭能力；Western blot分析检测AGS细胞真核细胞翻译起始因子4A3(EIF4A3)和血管内皮生长因子A(VEGFA)蛋白表达的变化。结果 LncRNA C2orf48表达在胃癌组织和细胞中显明显升高(P＜0.05)；与NC组比较,C2orf48组AGS细胞活力,G1期细胞百分率和侵袭细胞数降低,凋亡率升高(P＜0.05)；与NC组比较,C2orf48组AGS细胞EIF4A3和VEGFA表达均降低(P＜0.05)。结论 LncRNA C2orf48在胃癌组织和细胞中呈高表达。沉默LncRNA C2orf48抑制AGS细胞增殖和侵袭能力,并促进凋亡,其机制可能与抑制EIF4A3和VEGFA表达有关。 |
| 英文摘要: |
| Objective To investigate the effects of silencing long non-coding RNA C2orf48(LncRNA C2orf48)on the malignant behavior of human gastric cancer cell line AGS. Methods RT-qPCR was applied to detect the expression level of C2orf48 in gastric cancer tissues and gastric cancer cell lines. C2orf48 was silenced in AGS cells, which were categorized into the C2orf48 group, while untransfected cells were used as the negative control(NC) group. The CCK-8 assay was applied to detect AGS cell proliferation. Flow cytometry was applied to detect AGS cell apoptosis and cell cycle distribution. The Transwell invasion assay was applied to detect the invasion ability of AGS cells. Western blotting was applied to detect changes in the expression of eukaryotic translation initiation factor 4A3(EIF4A3) and vascular endothelial growth factor A(VEGFA) in AGS cells. Results The relative expression of LncRNA C2orf48 was significantly elevated in gastric cancer tissues and cells (P＜0.05). Compared with the NC group, the C2orf48 group reduced cell viability, cell population in the G1 phase, invasion cell number and increased apoptosis rate(P＜0.05). Additionally, compared with the NC group, the relative expressions of EIF4A3 and VEGFA were reduced in the C2orf48 group. Conclusion LncRNA C2orf48 is highly expressed in gastric cancer tissues and cells. Silencing LncRNA C2orf48 inhibits the proliferation and invasion abilities of AGS cells and promotes apoptosis. The underlying mechanism may be related to the suppression of EIF4A3 and VEGFA expression. |
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