| 张浩然,崔文杰,张旭艳.ADAM17、Notch1与子宫内膜异位症的上皮 -间质转化相关性研究[J].安徽医药,2025,29(3):550-554. |
| ADAM17、Notch1与子宫内膜异位症的上皮 -间质转化相关性研究 |
| Relationship between ADAM17, Notch1 and EMT in endometriosis |
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| DOI：10.3969/j.issn.1009-6469.2025.03.023 |
| 中文关键词: 子宫内膜异位症 上皮 -间质转化 去整合素 -金属蛋白酶 17 Notch1 E-钙黏蛋白 N-钙黏蛋白 ADAM17或靠激活EMT的r的发r |
| 英文关键词: Endometriosis Epithelial-mesenchymal transitions Disintegrin and metalloprotease 17 Notch1 E-cadherin N-cadherin |
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| 中文摘要: |
| 目的探讨去整合素 -金属蛋白酶 17(ADAM17)、Notch1在子宫内膜异位症(EMs)中的表达及其与上皮 -间质转化(EMT)的关系。方法选取 2018年 10月至 2022年 12月就诊于潍坊医学院附属医院患有 EMs卵巢异位囊肿者病人 40例，在行腹腔镜或开腹时剥除的囊壁内侧的异位出血病灶作为 EMs异位内膜组( n=40)，术中收取其在位子宫内膜作为 EMs在位内膜组( n= 40)选取同期因妇科其他良性疾病来院就诊的病人 40例，在行诊刮术或内膜活检时收取其子宫内膜作为正常子宫内膜组(对照组)，。采用免疫组织化学染色法检测 EMs异位内膜、在位内膜及正常子宫内膜组中 ADAM17、Notch1、E-钙黏蛋白、 N-钙黏蛋白的定位与表达情况；结果 ADAM17、Notch1、N-钙黏蛋白在 EMs异位内膜组表达评分 5.00(4.00，6.00)分、 4.5(4.0，5.0)分、 6.0(5.0，7.0)分最高， EMs在位内膜组表达评分 4.00(3.00，4.80)分、 4.0(3.0，5.0)分、 5.0(4.0，6.0)分次之，正常子宫内膜组表达评分 2.00(2.00，3.00)分、 3.0(2.0，4.0)分、 2.5(2.0，3.0)分最低，均差异有统计学意义( P<0.05)； E-钙黏蛋白在 EMs异位内膜组表达评分 1.0(1.0，2.0)分最低， EMs在位内膜组表达评分 3.0(2.0，4.0)分次之，正常子宫内膜组表达评分 5.5(5.0，6.0)分最高，均差异有统计学意义( P<0.05)；正常内膜组中各项表达评分均差异无统计学意义( P>0.05)； EMs异位内膜组中 ADAM17与 Notch1的表达呈正相关( rs=0.46，P=0.003)，Notch1与 N-钙黏蛋白的表达呈正相关( rs=0.38，P=0.014)E-钙黏蛋白与 ADAM17、Notch1的表达分别呈负相关( rs=.0.36，P=0.024；rs=.0.44，P=0.004)，余下均差异无统计学意义( P>0.05，)； EMs在位内膜组中， ADAM17与 Notch1、N-钙黏蛋白的表达分别呈正相关( rs=0.34，P=0.031； rs=0.61，P<0.001)Notch1与 N-钙黏蛋白的表达呈正相关( rs=0.63，P <0.001)； E-钙黏蛋白与 ADAM17、Notch1、N-钙黏蛋白的表达r均呈负相关( rs=0.51，P=0.001；rs=0.32，P=0.041；rs=0.52，P<0.001)；结论 ADAM17及 Notch1偏高表达可能是产生 EMs的重要原因； 过程促成 EMs生。 |
| 英文摘要: |
| Objective To explore the expressions of a disintegrin and metalloprotease 17 (ADAM17) and Notch1 in endometriosis(EMs), as well as their relationship with epithelial-to-mesenchymal transition (EMT).Methods Forty patients with endometriosis whowere treated in The Affiliated Hospital of Weifang Medical College from October 2018 to December 2022 were selected for the study.The ovarian endometriotic cysts on the inner wall of the uterus, which were performed laparoscopic or open surgery, were included inthe EMs ectopic endometrium group (n=40), the in situ endometriums collected during the procedures as the EMs eutopic endometrium group (n=40), and endometriums obtained during curettage or endometrial biopsy from other 40 patients who came to the hospital forother benign gynecological diseases during the same period as the normal endometrium group (control group). The localization and ex-pression levels of ADAM17, Notch1, E-cadherin and N-cadherin in ectopic endometrium, eutopic endometrium and normal endometri-um were detected by immunohistochemical staining. Results The expression scores of ADAM17, Notch1 and N-cadherin were the highest in the EMs ectopic endometrium group [5.00 (4.00, 6.00), 4.5 (4.0, 5.0), 6.0 (5.0, 7.0)], followed by those in the EMs eutopic en-dometrium group [4.00 (3.00, 4.80), 4.0 (3.0, 5.0), 5.0 (4.0, 6.0)], and the normal endometrium group [2.00 (2.00, 3.00), 3.0 (2.0, 4.0),2.5 (2.0, 3.0)], and the difference was statistically significant (P<0.05). The expression score of E-cadherin was the lowest in the EMs ectopic endometrium group [1.0 (1.0, 2.0)], that in the EMs eutopic endometrium group was [3.0 (2.0, 4.0)], and the highest was in thenormal endometrium group [5.5 (5.0, 6.0)]; the difference was statistically significant (P<0.05). There were no significant differences in expression scores in the normal endometrium group (P>0.05). In the EMs ectopic endometrium group, there was a positive correlation between the expressions of ADAM17 and Notch1 (r=0.46, P=0.003), a positive correlation between Notch1 and N-cadherin (r=0.38, P=0.014), and a negative correlation between E-cadherin and ADAM17 and Notch1, respectively (r=.0.36, P=0.024; r=.0.44, P=0.004). There were no significant correlations between other expressions (P>0.05); in the EMs eutopic endometrium group, the expression of ADAM17 was positively correlated with Notch1 and N-cadherin, respectively (r=0.34, P=0.031; r=0.61, P<0.001), the expression of Notch1 was positively correlated with N-cadherin (r=0.63, P<0.001), while the expression of E-cadherin was negatively correlated with ADAM17, Notch1, and N-cadherin, respectively (r=.0.51, P=0.001; r=.0.32, P=0.041; r=.0.52, P<0.001).Conclusion The higherexpressions of ADAM17 and Notch1 may be an important cause of EMs. ADAM17 promotes the progress of EMs by activating EMT. |
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