| 邵宪萍,金小乐,张丹,等.慢性阻塞性肺疾病急性加重病人血清组蛋白 H3与外周血 NLRP3炎症小体表达的相关性[J].安徽医药,2025,29(3):570-574. |
| 慢性阻塞性肺疾病急性加重病人血清组蛋白 H3与外周血 NLRP3炎症小体表达的相关性 |
| Correlation between serum histone H3 and peripheral blood NLRP3 inflammasome expression in patients with AECOPD and its clinical significance |
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| DOI：10.3969/j.issn.1009-6469.2025.03.027 |
| 中文关键词: 慢性阻塞性肺疾病急性加重期 组蛋白 H3 Nod样受体蛋白 3 预后 |
| 英文关键词: Acute exacerbation of chronic obstructive pulmonary disease Histone H3 NLRP3 Prognosis |
| 基金项目:河北省医学科学研究基金项目( 20231451)；河北省 2019年度医学科学研究课题计划项目( 20190902) |
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| 中文摘要: |
| 目的研究慢性阻塞性肺疾病急性加重( AECOPD)病人血清组蛋白 H3与外周血 Nod样受体蛋白 3(NLRP3)炎症小体表达的相关性及其临床意义。方法选择 2020年 1月至 2021年 5月河北北方学院附属第一医院收治的 AECOPD病人作为 AECOPD组( n=105)、稳定期慢性阻塞性肺疾病( COPD)病人作为稳定期 COPD组(n=120)另取同期体检的健康志愿者作为健康组( n=100)。检测血清组蛋白 H3含量及外周血 NLRP3、凋亡相关斑点样蛋白( ASC)、胱天，蛋白酶 -1(caspase-1)的 mRNA相对表达水平；随访 AECOPD病人出院后 1年的预后情况。结果 AECOPD组的血清组蛋白 H3含量及外周血 NLRP3、ASC、cas-pase-1表达水平均高于稳定期 COPD组及健康组［( 8.39±2.26)μg/L比( 6.11±1.58)μg/L、(3.42±0.94)μg/L，1.40±0.41比( 1.12±0.37、1.00±0.24)，1.28±0.24比( 1.12±0.32、1.00±0.27)，1.21±0.28比( 1.11±0.30、1.00±0.22)］(P<0.05)； AECOPD组中预后不良病人的血清组蛋白 H3含量及外周血 NLRP3、ASC、caspase-1表达水平均高于预后良好病人［( 10.27±1.66)μg/L比( 7.41±1.88)μg/ L，1.49±0.32比 1.38±0.41，1.40±0.35比 1.21±0.27，1.33±0.24比 1.14±0.28］(P<0.05)；血清组蛋白 H3含量及外周血 ASC、caspase-1表达水平对 AECOPD具有诊断价值、对 AECOPD预后具有评估价值。结论 AECOPD发病过程中组蛋白 H3释放增加与 NL-RP3炎症小体激活可能共同参与了 AECOPD的发病及预后不良。 |
| 英文摘要: |
| Objective To study the correlation between serum histone H3 and peripheral blood Nod-like receptor protein 3(NLRP3)inflammasome expression in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and its clinical sig-nificance.Methods One hundred and five patients with AECOPD treated in the First Affiliated Hospital of Hebei North Universityfrom January 2020 to May 2021 were selected as AECOPD group, 120 patients with stable chronic obstructive pulmonary disease(COPD) as stable COPD group, and healthy volunteers who underwent physical examination at the same time as the healthy group. Thecontent of serum histone H3 and the relative mRNA expression levels of NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC) and cysteinyl aspartate specific proteinase-1 (caspase-1) in peripheral blood were detected. The prognosis of AECOPD pa-tients one year after discharge was followed up.Results The content of serum histone H3 and the expression levels of NLRP3, ASC and caspase-1 in peripheral blood of AECOPD group were higher than those of stable COPD group and healthy group [(8.39±2.26) μg/L vs. (6.11±1.58) μg/L, (3.42±0.94) μg/L, 1.40±0.41 vs. (1.12±0.37, 1.00±0.24), 1.28±0.24 vs. (1.12±0.32, 1.00±0. 27), 1.21±0.28 vs. (1.11±0.30, 1.00±0.22)] (P<0.05). In AECOPD group, the content of serum histone H3 and the expression levels of NLRP3, ASC and caspase-1 in peripheral blood of patients with poor prognosis were higher than those of patients with good prognosis[(10.27±1.66) μg/L vs. (7.41±1.88) μg/L, 1.49±0.32 vs. 1.38±0.41, 1.40±0.35 vs. 1.21±0.27, 1.33±0.24 vs. 1.14±0.28]. The content of serum histone H3 and the expression levels of ASC and caspase-1 in peripheral blood had diagnostic value and prognostic evaluation value for AECOPD. Conclusion The increased release of histone H3 and the activation of NLRP3 inflammasome in the pathogenesis of AECOPD may beinvolved in the pathogenesis and poor prognosis of AECOPD. |
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