| 刘磊,王亚丽,胡景玉.丰富环境通过海马 BDNF相关通路减轻社会隔离所致认知功能损伤[J].安徽医药,2025,29(4):687-691. |
| 丰富环境通过海马 BDNF相关通路减轻社会隔离所致认知功能损伤 |
| Influence of enriched environment on cognitive impairment induced by social isolation through hippocampal BDNF-related pathways |
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| DOI：10.3969/j.issn.1009-6469.2025.04.010 |
| 中文关键词: 丰富环境 海马 脑源性神经生长因子 /原肌球蛋白相关激酶 B信号通路( BDNF/TrkB信号通路) 社会隔离 认知功能受损 |
| 英文关键词: Enriched environment Seahorse BDNF/TrkB signaling pathways Social isolation Cognitive impairment |
| 基金项目:河北医学科学研究重点课题计划项目( 20181588) |
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| 中文摘要: |
| 目的探究丰富环境对社会隔离所致认知功能损伤的影响及其与海马脑源性神经生长因子( BDNF)相关通路的关系。方法 2023年 1—4月，将 30只小鼠按随机数字表法分为对照组( NC组)、模型组( SI组)和治疗组( SI+EE组)每组 10只。 NC组以每笼 5只饲养于标准环境中， SI组和 SI+EE组以每笼 1只分别饲养于标准环境和丰富环境中， 4周后检测小，鼠认知功能和学习记忆能力；免疫组织化学荧光染色和蛋白质印迹法检测海马组织中 BDNF和原肌球蛋白相关激酶 B(TrkB)蛋白表达；逆转录聚合酶链式反应( RT-PCR)检测海马组织中 BDNF和 TrkB mRNA表达。结果 NC组新事物分辨率为( 32.68±5.55)%、新位置分辨率为( 19.56±3.92)%、目标象限停留时间为( 47.12±7.43)s、穿越平台次数为( 4.63±0.54)次，均显著高于 SI组的( 10.89±3.57)%、(2.78±0.36)%、(30.92±2.95)s、(1.38±0.15)次， NC组逃避潜伏期显著低于 SI组( P<0.05)；和 SI组相比， SI+EE组小鼠新事物分辨率、新位置分辨率、目标象限停留时间和穿越平台次数明显升高，逃避潜伏期明显降低( P<0.05)。 NC组 BDNF、TrkB mRNA表达量显著高于 SI组的( P<0.05)；和 SI组相比， SI+EE组小鼠海马组织中 BDNF、TrkB蛋白和 mRNA表达明显升高( P<0.05)。结论丰富环境可减轻社会隔离所致小鼠的认知功能损伤，其可能和调节 BDNF/TrkB信号转导通路有关。 |
| 英文摘要: |
| Objective Exploring the impact of enriched environment on cognitive impairment caused by social isolation and its rela.tionship with the hippocampal brain-derived neurotrophic factor (BDNF) related pathway. Methods From January 2023 to April2023, 30 mice were randomly assigned into a control group (NC group), a model group (SI group), and a treatment group (SI+EE group)according to numerical methods, each group consisting of 10 mice. The NC group was housed in groups of 5 in a standard environment,while the SI and SI+EE groups were individually housed in a standard and enriched environment, respectively. After 4 weeks, cognitive function and learning and memory abilities of the mice were assessed. Immunofluorescence staining and Western blot analysis wereused to detect BDNF and Tropomyosin-related kinase B (TrkB) protein expression in hippocampal tissue. Reverse transcription poly. merase chain reaction (RT-PCR) was used to measure BDNF and TrkB mRNA expressions in hippocampal tissue. Results In the NC group, the novel object recognition, novel location recognition, target quadrant dwell time and times across the platform in NC groupwere significantly higher than those in SI group. [(32.68±5.55) % vs. (10.89±3.57) %, (19.56±3.92) % vs. (2.78±0.36) %, (47.12±7.43) s vs. (30.92±2.95) s, (4.63±0.54) times vs. (1.38±0.15) times, respectively]. The escape latency in NC group was significantly shorter than that in SI group (P<0.05). Compared with the SI group, the SI+EE group showed significantly increased performances in novel objectrecognition, novel location recognition, target quadrant dwell time and times across the platform, as well as decreased escape latency (P <0.05). The mRNA expression levels of BDNF and TrkB in NC group were significantly higher than those in SI group (P<0.05), whilecompared with SI group, the SI+EE group showed significantly increased protein and mRNA expression levels of BDNF and TrkB inhippocampal tissue (P<0.05).Conclusion Enriched environment can improve cognitive impairment in socially isolated mice, whichmay be related to the regulation of BDNF/TrkB signaling pathway. |
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