| 褚丽芳,朱倩,姚洁,等.血清 mir-223、HMGB1水平与脑卒中后并发血管性认知功能障碍的关系[J].安徽医药,2025,29(5):967-972. |
| 血清 mir-223、HMGB1水平与脑卒中后并发血管性认知功能障碍的关系 |
| The relationship between serum mir-223 and HMGB1 levels and vascular cognitive impairment after stroke |
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| DOI：10.3969/j.issn.1009-6469.2025.05.022 |
| 中文关键词: 高迁移率族蛋白 B1 微 RNA-223 脑卒中 血管性认知功能障碍 |
| 英文关键词: High mobility group protein B1 Micro RNA-223 Stroke Vascular cognitive impairment |
| 基金项目:石家庄市科学技术研究与发展计划项目( 211201313) |
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| 中文摘要: |
| 目的探讨血清微 RNA-223(mir-223)、血清高迁移率族蛋白 B1(HMGB1)水平与脑卒中后并发血管性认知功能障碍(VCI)的关系。方法选取 2021年 8月至 2022年 12月石家庄市人民医院就诊的 100例脑卒中病人作为研究对象，根据病人是否并发 VCI分为发生 VCI组 31例和未发生 VCI组 69例。采用实时荧光定量逆转录聚合酶链式反应( qRT-PCR)检测脑卒中病人血清 mir-223水平，酶联免疫吸附分析测定血清 HMGB1水平，并对两组病人进行 NIHSS评分。分析血清 mir-223、HMGB1水平与病人临床病理特征的关系； Pearson法分析脑卒中病人血清 mir-223与 HMGB1水平的关系； Spearman相关性分析血清 mir-223、HMGB1水平与 NIHSS评分的关系；采用多因素 logistic回归分析脑卒中病人并发 VCI的影响因素； ROC曲线评估血清 mir-223、HMGB1水平对脑卒中后是否并发 VCI的预测价值。结果脑卒中后发生 VCI组血清 mir-223(1.91±0.20比 2.15±0.22)显著低于未发生 VCI组， HMGB1水平［( 5.63±0.57)μg/L比( 5.34±0.54)μg/L］、 NIHSS评分［(15.41±2.65)分比( 3.02±0.57)分］均显著高于未发生 VCI组( P<0.05)；轻度、中度、重度 VCI病人血清 mir-223水平依次降低、 HMGB1水平依次升高，三组间比较均差异有统计学意义( P<0.05)。脑卒中病人血清 mir-223与 HMGB1水平呈负相关( r=.0.33，P<0.001)；血清 mir-223水平与 NI-HSS评分呈负相关， HMGB1水平与 NIHSS评分呈正相关( P<0.05)；脑卒中后发生 VCI组病人 Hcy、CRP水平及高血压、冠心病和心房颤动比例显著高于未发生 VCI组( P<0.05)； mir-223、HMGB1、Hcy和 CRP是脑卒中后并发 VCI的影响因素( P<0.05)；血清 mir-223、HMGB1联合预测脑卒中后 VCI发生的 AUC为 0.89，优于单项检测( Zmir-223-联合 =2.40，P=0.016；ZHMGB1-联合 =2.09，P=0.037)。结论血清 mir-223、HMGB1水平与脑卒中后并发 VCI的关系密切，为发生 VCI的影响因素，对预测脑卒中后并发 VCI具有重要价值。 |
| 英文摘要: |
| Objective To investigate the relationship between serum levels of microRNA-223 (mir-223) and high mobility group pro-tein B1 (HMGB1) and vascular cognitive impairment (VCI) after stroke.Methods A total of 100 stroke patients who visited the Peo-ple's Hospital of Shijiazhuang from August 2021 to December 2022 were collected as the study subjects.They were grouped into a VCIgroup of 31 patients and a non VCI group of 69 patients based on whether they had concurrent VCI. QRT-PCR method was applied to detect serum mir-223 level in stroke patients, enzyme-linked immunosorbent assay (ELISA) was applied to measure serum HMGB1 lev-el, and NIHSS score was performed on two groups. The relationship between serum mir-223, HMGB1 levels and clinical pathologicalcharacteristics of patients was analyzed; Pearson method was applied to analyze the relationship between serum mir-223 and HMGB1 levels in stroke patients; Spearman correlation was applied to analyze the relationship between serum mir-223, HMGB1 levels and NI-HSS score; multiple logistic regression was applied to analyze the influencing factors of VCI in stroke patients; ROC curve was appliedto evaluate the predictive value of serum mir-223 and HMGB1 levels for the occurrence of VCI after stroke.Results The level of se-rum mir-223(1.91±0.20 vs. 2.15±0.22) in the group with VCI after stroke were obviously lower than those in the group without VCI, andthe level of HMGB1[(5.63±0.57)μg/L vs. (5.34±0.54)μg/L] and NIHSS score[(15.41±2.65)points vs. (3.02±0.57)points] were obviously higher than those in the group without VCI (P<0.05); the serum mir-223 level in patients with mild, moderate, and severe VCI de-creased in sequence, while HMGB1 level increased in sequence (P<0.05). There was a negative correlation between serum mir-223 and HMGB1 levels in stroke patients (r=. 0.33, P<0.01); the serum mir-223 level was negatively correlated with NIHSS score, while the HMGB1 level was positively correlated with NIHSS score (P<0.05); the levels of Hcy and CRP, and the proportions of hypertension, cor-onary heart disease, and atrial fibrillation in patients with VCI after stroke were obviously higher than those in patients without VCI (P< 0.05); mir-223, HMGB1, Hcy, and CRP were the influencing factors of VCI after stroke (P<0.05); the combination of serum mir-223 and HMGB1 had an AUC of 0.89 for predicting occurrence of VCI after stroke, which was superior to single detection (Zmir-223-combination = 2.40,P=0.016; ZHMGB1-combination=2.09,P=0.037).Conclusion The levels of serum mir-223 and HMGB1 are closely related to the occur-rence of VCI after stroke, and they are influencing factors for the occurrence of VCI.They have important value in predicting the occur-rence of VCI after stroke. |
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