| 王俊玲,杨春菊,李秀萍,等.紫草素延缓秀丽隐杆线虫衰老的实验研究[J].安徽医药,2025,29(6):1127-1132. |
| 紫草素延缓秀丽隐杆线虫衰老的实验研究 |
| An experimental study on the antiaging effects of shikonin on Caenorhabditis elegans |
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| DOI：10.3969/j.issn.1009-6469.2025.06.012 |
| 中文关键词: 紫草素 秀丽隐杆线虫 自噬 衰老 寿命 |
| 英文关键词: Shikonin Caenorhabditis elegans Antiaging Autophagy Longevity |
| 基金项目:云南省教育厅科学研究基金项目( 2022J0226，2022J0230)；云南省科技厅科技计划项目( 202201AY070001-214，202301AY070001-071) |
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| 中文摘要: |
| 目的研究紫草素延缓秀丽隐杆线虫衰老的功效及其作用机制。方法 2021年 5月至 2022年 12月，给予秀丽隐杆线虫不同浓度的紫草素( 300 μmol/L、600 μmol/L和 1 200 μmol/L)处理，通过测定秀丽隐杆线虫的寿命和衰老相关表型，以及高浓度紫草素处理下线虫的消化能力、抗高温逆性和抗氧化能力，评价了紫草素延长线虫寿命抗衰老的功效；通过实时荧光定量逆转录聚合酶链反应( qRT-PCR)检测胶原相关基因表达量以及 RNA干扰实验沉默自噬基因 bec-1和 let-512，并初步探讨了紫草素延缓衰老的作用机制。结果与对照组线虫相比，浓度 300 μmol/L和 600 μmol/L的紫草素可以通过自噬延长实验组线虫寿命［最长至( 70±5)d比( 52±3)d］600 μmol/L的紫草素可以增加线虫的吞咽频率［( 105.32±3.26)次 /30 s比( 93.68±3.15)次 /30 s］和身体弯曲频率［( 39.36±5.68/30 s比( 30.16±4.86)次 /30 s)］、高温逆性环境中的存活率［( 57.67±9.29)%比( 40.33± )次，5.03)%］以及氧化应激下的存活率［( 43.00±4.58)%比( 30.67±5.51)%］减慢咽部退化进程，使评分为 1分的线虫比率增加［( 54.00±4.00)%比( 36.67±3.06)%］降低肠道泄漏率［( 25.00±3.61)%比0.67±3.79)%］上调胶原基因 col-10(1.117±0.074比(5，0.487±0.025)、 col-65(0.887±0.025比0.683±0.031)、 col-144(0.920±0.056比 0.453±0.035)、dyp-4(0.760±0.056比 0.543±0.072)和 dyp-13(0.697±0.050比 0.320±0.078)的表达量。结论紫草素可以通过自噬维持线虫体内胶原基因表达水平，稳定线虫消化系统结构，提高线虫面对氧化胁迫及急性热激的应激能力，延缓线虫衰老。 |
| 英文摘要: |
| Objective This study aimed to investigate the antiaging effects of shikonin on Caenorhabditis elegans and explore its un-derlying mechanisms.Methods From May 2021 to December 2022, Caenorhabditis elegans were treated with varying concentrations of shikonin (300 μmol/L, 600 μmol/L, and 1 200 μmol/L). Lifespan and aging-related phenotypes were measured. Antiaging effectswere evaluated by assessing digestive capacity, heat resistance, and antioxidant ability at high concentrations. Quantitative real-time fluorescence PCR was used to detect collagen-related genes expression. RNA interference experiments silenced autophagy-related genes bec-1 and let-512 to preliminary investigate shikonin's mechanism for delaying aging.Results Compared to the control group,shikonin at 300 μmol/L and 600 μmol/L extended the lifespan of treated nematodes via autophagy [(70±5) days vs. (52±3) days]. The 600 μmol/L concentration increased swallowing frequency [(105.32±3.26) times/30s vs. (93.68±3.15) times/30s] and body bending fre-quency [(39.36±5.68) times/30s vs. (30.16±4.86) times/30s]. It also enhanced survival rates under high-temperature stress [(57.67± 9.29)% vs. (40.33±5.03)%] and oxidative stress [(43.00±4.58)% vs. (30.67±5.51)%)], slowed pharyngeal degeneration [(54.00±4.00)% vs. (36.67±3.06)% with score 1)], reduced intestinal leakage [(25.00±3.61)% vs. (50.67±3.79)%], and upregulated collagen gene expres-sion: col-10 (1.117±0.074 vs. 0.487±0.025), col-65 (0.887±0.025 vs. 0.683±0.031), col-144 (0.920±0.056 vs. 0.453±0.035), dyp-4 (0.760±0.056 vs. 0.543±0.072), and dyp-13 (0.697±0.050 vs. 0.320±0.078).Conclusion Shikonin maintains collagen genes expres-sion through autophagy, stabilizes digestive system structure, enhances stress responses to oxidative stress and acute heat shock, anddelays aging of in Caenorhabditis elegans. |
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