| 郭健,曾友玲,余莉.山柰酚抑制 AKT/mTOR信号途径逆转宫颈癌 HeLa细胞的恶性行为[J].安徽医药,2025,29(7):1315-1318. |
| 山柰酚抑制 AKT/mTOR信号途径逆转宫颈癌 HeLa细胞的恶性行为 |
| Kaempferol reverses the malignant behavior of cervical cancer HeLa cells by inhibiting AKT/mTOR signal pathway |
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| DOI：10.3969/j.issn.1009-6469.2025.07.010 |
| 中文关键词: 山柰酚 宫颈肿瘤 蛋白激酶 B/哺乳动物雷帕霉素靶蛋白信号通路 黏附 迁移 侵袭 |
| 英文关键词: Kaempferol Uterine cervical neoplasms Protein kinase B/ mammalian target of rapamycin signaling pathway Adhe-sion Migration Invasion |
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| 中文摘要: |
| 目的探究山柰酚对宫颈癌细胞黏附、迁移、侵袭及蛋白激酶 B/哺乳动物雷帕霉素靶蛋白( AKT/mTOR)信号通路的调控作用。方法该研究起止时间为 2021年 12月至 2022年 12月。体外培养人宫颈癌 HeLa细胞，分为对照组、 10 μmol/L山柰酚组、 20 μmol/L山柰酚组、 40 μmol/L山柰酚组和 80 μmol/L山柰酚组，筛选出最合适山柰酚的浓度( 40 μmol/L)后又分为五组(对照组、 40 μmol/L山柰酚组、阳性药物组、抑制剂组、激活剂组)，各组处理 24 h。分析各种细胞黏附(细胞黏附实验)、迁移(划痕实验)、侵袭( Transwell小室)能力及 AKT/mTOR信号通路相关蛋白(蛋白免疫印迹法)表达水平。结果选取 40 μmol/L山柰酚进行后续实验。 40 μmol/L山柰酚组、阳性药物组细胞黏附数、迁移率、侵袭数、磷酸化( p)-AKT、p-mTOR蛋白表达水平低于对照组( P<0.05)；抑制剂组细胞黏附数、迁移率、侵袭数及 p-AKT蛋白表达水平( 0.24±0.02比 0.40±0.01)、 p-mTOR蛋白表达水平( 0.21±0.01比 0.37±0.02)较 40 μmol/L山柰酚组下调( P<0.05)，激活剂组上述指标较 40 μmol/L山柰酚组上调( P<0.05)。结论山柰酚对宫颈癌细胞(HeLa)黏附、迁移和侵袭具有抑制作用，其调控机制可能依赖于对 AKT/mTOR通路的抑制。 |
| 英文摘要: |
| Objective To explore the regulatory effects of kaempferol on the adhesion, migration, invasion and the protein kinase B/mammalian target of rapamycin (AKT/mTOR) signaling pathway of cervical cancer cells.Methods The study was conducted from De-cember 2021 to December 2022. HeLa cells from human cervical cancer were cultured in vitro and divided into control group, 10 μmol/L kaempferol group, 20 μmol/L kaempferol group, 40 μmol/L kaempferol group and 80 μmol/L kaempferol group. After the most suit-able concentration of kaempferol (40 μmol/L) was selected, the cells were divided into 5 groups (control group, 40 μmol/L kaempferolgroup, positive drug group, inhibitor group and activator group), and each group was treated for 24 hours. Cell adhesion (cell adhesion as-say), migration (scratch assay), invasion (Transwell chamber) and the expression levels of AKT/mTOR signaling pathway-related proteins (Western blotting) were analyzed.Results 40 μmol/L kaempferol was selected for subsequent experiments. The number of cell adhe-sion, migration rate, invasion, and the expression levels of phosphorylated (p) -AKT and p-mTOR protein in the 40 μmol/L kaempferol group and the positive drug group were lower than those in the control group (P<0.05). The number of cell adhesion, migration rate, inva-sion, p-AKT protein expression [(0.24±0.02) vs. (0.40±0.01)] and p-mTOR protein expression [(0.21±0.01) vs. (0.37±0.02)] in the inhibi-tor group were lower than those in the 40 μmol/L kaempferol group (P<0.05). Compared with the 40 μmol/L kaempferol group, the above indicators in the activator group were up-regulated (P<0.05).Conclusion Kaempferol can inhibit the adhesion, migration and invasionof HeLa cells (cervical cancer cells), whose regulatory mechanism may depend on the inhibition of AKT/mTOR pathway. |
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