| 陈铖,詹观明,葛丹丹,等.黄芪多糖对小鼠癌因性疲乏的保护作用及机制研究[J].安徽医药,2025,29(8):1509-1512. |
| 黄芪多糖对小鼠癌因性疲乏的保护作用及机制研究 |
| Protective effect and mechanism of Astragalus polysaccharides on cancer-induced fatigue in mice |
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| DOI：10.3969/j.issn.1009-6469.2025.08.005 |
| 中文关键词: 黄芪 疲劳 黄芪多糖 癌因性疲乏 肝糖原 血尿素氮 乳酸 |
| 英文关键词: Astragalus membranaceus Fatigue Astragalus polysaccharide Cancer-related fatigue Liver glycogen Blood urea nitrogen Lactic acid |
| 基金项目:安徽省中医药传承创新科研项目重点项目( 2024CCCX019) |
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| 中文摘要: |
| 目的探讨黄芪多糖对小鼠癌因性疲乏( CRF)的改善作用。方法 2023年 6—12月将 30只雌性 SPF级 BALB/c小鼠以随机数字表法分为正常对照组，模型组，黄芪多糖高、中、低剂量组，每组 6只。除正常对照组外其余各组小鼠腋下注射 4T1细胞构建荷 4T1肿瘤三阴性乳腺癌( TNBC)小鼠模型，给药 12 d后对各组小鼠进行爬杆实验，给药 14 d后对各组小鼠进行负重游泳实验，在负重游泳实验结束后 1h检测小鼠血清中血尿素氮( BUN)、乳酸水平及肝脏中肝糖原水平；检测小鼠肿瘤组织体积、体质量及肿瘤组织中凋亡相关蛋白表达量。结果黄芪多糖中剂量组( 7.15±0.75)min、高剂量组( 7.80±0.97)min的负重游泳时间显著高于模型组( 5.75±0.45)min，黄芪多糖中剂量组( 202.50±9.48)min、高剂量组( 221.00±10.18)min的爬杆时间显著高于模型组( 181.33±15.24)min；黄芪多糖可改善荷瘤小鼠的肝糖原消耗，降低血清中尿素氮和乳酸的含量，抑制肿瘤的生长，通过 Bcl-2相关 X蛋白( Bax)B细胞淋巴瘤 -2(Bcl-2)促进肿瘤的凋亡。结论黄芪多糖可通过改善肝糖原的消耗、蛋白质的水解代谢和乳酸的产生，促进肿瘤凋亡从而缓解疲乏。 |
| 英文摘要: |
| Objective To investigate the effect of Astragalus polysaccharide (APS) on cancer-induced fatigue (CRF) in mice.Meth. ods From June 2023 to December 2023, thirty female SPF BALB/c mice were assigned into normal control group, model group andAstragalus polysaccharide high-dose, medium-dose and low-dose groups based on random number table, with 6 mice in each group. Inaddition to the normal control group, the other groups of mice were injected with 4T1 cells under the armpit to build a 4T1 triple nega-tive breast cancer (TNBC) mouse model. After 12 days of administration, the mice in each group were subjected to pole climbing experi-ment, and after 14 days of administration, the mice in each group were subjected to weight-bearing swimming experiment. The levels ofurea nitrogen (BUN) and lactic acid (LD) in serum and liver glycogen in mice were detected 1 h after the weight-bearing swimming ex.periment.Results The swimming time to exhaustion in the medium-dose group of Astragalus polysaccharide (7.15±0.75) min and the high-dose group (7.80±0.97) min was significantly longer than that in the model group (5.75±0.45) min. The climbing time in the medi-um-dose group (202.50±9.48) min and the high-dose group (221.00±10.18) min was significantly longer than that in the model group(181.33±15.24) min. Astragalus polysaccharide improved the liver glycogen consumption, decreased the content of urea nitrogen andlactic acid in serum, inhibited tumor growth, and promoted tumor apoptosis through Bcl-2 associated X protein (Bax) B cell lymphoma-2 (Bcl-2).Conclusion Astragalus polysaccharide can alleviate fatigue by improving liver glycogen consumption, protein hydrolytic me-tabolism and lactic acid production. |
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