| 胡灵利,樊卫平.纤维蛋白黏合剂负载地塞米松调节伤口炎症加速愈合进程的研究[J].安徽医药,2025,29(8):1513-1518. |
| 纤维蛋白黏合剂负载地塞米松调节伤口炎症加速愈合进程的研究 |
| Fibrin adhesive loaded with dexamethasone adjust wound inflammation and accelerated healing process |
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| DOI：10.3969/j.issn.1009-6469.2025.08.006 |
| 中文关键词: 组织黏合剂 纤维蛋白 地塞米松 伤口愈合 组织再生 炎症反应 |
| 英文关键词: Tissue adhesives Fibrin Dexamethasone Wound closure Tissue regeneration Inflammatory response |
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| 中文摘要: |
| 目的探究纤维蛋白黏合剂( Fibrin)负载地塞米松( Dex)后对皮肤伤口愈合的影响。方法 2022年 1—10月，该研究将成年 SD雄性大鼠 48只按随机数字表法分成生物毒性组和皮肤全层缺损组，每组各 24只，每组再按随机数字表法分为对照组、 Dex组、 Fibrin组和 Fibrin+Dex组，每个亚组各 6只。在 SD大鼠后背创建直径 1 cm的皮肤全层缺损模型，对伤口拍照并计算术后第 7天和第 14天的伤口闭合率，通过苏木精 -伊红( HE)染色和免疫组织化学染色对再生皮肤组织进行病理学分析。统计肉芽厚度和肿瘤坏死因子 α(TNF-α)表达；通过羟脯氨酸试剂盒分析再生胶原的沉积水平；采用蛋白质印迹法检测转化生长因子 β(TGF-β)和血管内皮生长因子( VEGF)表达量。用皮下埋植实验验证 Fibrin+Dex的生物毒性。结果 Dex组毒性Fibrin组毒性和 Fibrin+Dex组毒性与正常肝脏组织的病理学切片基本一致。 Fibrin+Dex组在第 7天(69.2±2.1)%和第 14天( 98.1±2.7)、%的伤口闭合率显著高于对照组［( 39.2±3.6)%、(77.6±4.4)%］、 Dex组［( 48.9±4.1)%、(83.3±3.4)%］、 Fibrin组［( 57.1±3.1)%、(92.8±3.0)%］。 Fibrin+Dex组的 TNF-α表达量较低，表明抑制了炎症反应，同时， TGF-β和 VEGF水平分别是 Dex组的 2.1倍和 Fibrin组的 1.4倍，且肉芽组织更厚，羟脯氨酸水平更高。结论 Fibrin通过负载地塞米松，可以降低伤口愈合的炎症反应，从而加速伤口愈合进程。 |
| 英文摘要: |
| Objective To explore the effects of Fibrin loading dexamethasone on wound healing.Methods From January to October 2022, 48 adult male SD rats were randomly divided into a biotoxicity group and a full-thickness skin defect group using a random num-ber table method, with 24 rats in each group. Each group was further divided into a control group, Dex group, Fibroin group, and Fibro-in+Dex group using a random number table method, with 6 rats in each subgroup. A full-thickness skin defect model with a diameter of 1 cm was created on the back of SD rats. The wound closure for each group was measured on day 7 and 14 after the operation. The path-ological sections of HE and immunohistochemical staining were used to evaluate the regenerated skin tissues. Granulation thicknessand tumor necrosis factor-α (TNF-α) expression were measured. Hydroxyproline kit was used to analyze collagen deposition levels in re-generated skin tissues. The contents of transforming growth factor-β (TGF-β) and vascular endothelial growth factor (VEGF) were de-tected by western blotting.Validation of the biological toxicity of Fibroin+Dex through subcutaneous implantation experiment.Results Dex grouptoxicity, Fibroin grouptoxicity and Fibroin+Dex grouptoxicity is basically consistent with the pathological sections of normal liver tissue.The wound closure rates of the Fibroin+Dex group on day 7 (69.2±2.1)% and day 14 (98.1±2.7)% were significantly higher than thoseof the control group [(39.2±3.6)% , (77.6±4.4)% ], Dex group [(48.9±4.1)% , (83.3±3.4)% ], and Fibroin group [(57.1±3.1)% , (92.8±3.0)%]. The expression of TNF-α for Fibrin+Dex group was lower, indicating that the inflammatory response was suppressed. Addition-ally, the levels of TGF-β and VEGF in the Fibrin+Dex group were 2.1 times and 1.4 times, which were higher than those in the Dexgroup and the Fibrin group respectively, and the granulation tissue of Fibrin+Dex group was thicker with a higher hydroxyproline levelat than in other groups.Conclusion Fibrin loading dexamethasone can reduce the inflammatory reaction to accelerate the wound heal-ing process. |
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