| 贾冠美,曹朗,王江涛,等.环状 RNA_001209对高糖诱导视网膜神经节细胞凋亡的影响[J].安徽医药,2025,29(8):1550-1555. |
| 环状 RNA_001209对高糖诱导视网膜神经节细胞凋亡的影响 |
| Effect of Circ_001209 on high-glucose-induced apoptosis of retinal ganglion cells by regulating the miR-138-5p/SOX7 axis |
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| DOI：10.3969/j.issn.1009-6469.2025.08.013 |
| 中文关键词: 糖尿病视网膜病变 环状 RNA_001209 微 RNA-138-5p/SRY盒相关基因 7轴 高血糖 视网膜神经节细胞 细胞凋亡 |
| 英文关键词: Diabetic retinopathy Circ_001209 miR-138-5p/SOX7 axis Hyperglycemia Retinal ganglion cells Apoptosis |
| 基金项目:保定市科技计划项目( 2141ZF218) |
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| 中文摘要: |
| 目的探讨环状 RNA(Circ)_001209调节微 RNA(miR)-138-5p/SRY盒相关基因 7(SOX7)轴对高糖诱导视网膜神经节细胞凋亡的影响。方法实时荧光定量逆转录聚合酶链反应( qRT-PCR)检测 2022年 9月至 2023年 9月保定市第二中心医院眼科诊室接收的 30例糖尿病性视网膜病变( DR)病人及同期健康体检人员血清中 Circ_001209、miR-138-5p、SOX7 mRNA表达；取生长良好的视网膜神经节 RGC-5细胞，设置空白组、高糖组、高糖 +小干扰 RNA(si)NC组、高糖 +si Circ_001209组、高糖 +si Circ_001209+miRNA抑制剂( inhibitor)NC组、高糖 +si Circ_001209+miR-138-5p inhibitor组；噻唑蓝( MTT)、流式细胞仪检测 RGC-5细胞增殖及凋亡； qRT-PCR检测 RGC-5细胞 Circ_001209、miR-138-5p、SOX7 mRNA表达；双萤光素酶验证 miR-138-5p与 Circ_001209、SOX7的靶向关系；蛋白质印迹法检测 SOX7、B淋巴细胞瘤 -2(Bcl-2)、 Bcl-2相关 X蛋白( Bax)表达。结果 DR病人血清中 Circ_001209、SOX7 mRNA表达显著增加， miR-138-5p表达显著降低( P<0.05)。与空白组相比，高糖组凋亡率［( 32.24±3.24)%比( 7.04±0.71)%］、 Circ_001209、SOX7 mRNA及蛋白、 Bax蛋白表达显著增加；增殖率［( 44.01±4.41)%比(94.25±5.18)%］、 miR-138-5p、Bcl-2蛋白表达显著降低( P<0.05)；与高糖 +si NC组相比，高糖 +si Circ_001209组凋亡率［(17.34±1.74)%比( 32.11±3.22)%］、 Circ_001209、SOX7 mRNA及蛋白、 Bax蛋白表达显著降低，增殖率［( 78.28±7.84)%比( 43.87±4.39)%］、 miR-138-5p、Bcl-2蛋白表达显著增加( P<0.05)； miR-138-5p inhibitor则逆转了干扰 Circ_001209对高糖诱导 RGC-5细胞的保护作用。 miR-138-5p与 Circ_001209、SOX7具有靶向调节关系。结论干扰 Circ_001209可抑制高糖诱导视网膜神经节细胞凋亡，减轻高糖诱导 RGC-5细胞受损，机制可能与上调 miR-138-5p/SOX7轴有关。 |
| 英文摘要: |
| Objective To investigate the effect of CircRNA (Circ)_001209 on high-glucose-induced apoptosis of retinal ganglion cells by regulating the microRNA (miR)-138-5p/sex-determining region Y-box 7 (SOX7) axis.Methods Real-time fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression levels of Circ_001209, miR-138-5p andSOX7 mRNA in serum from 30 patients with diabetic retinopathy (DR) and healthy individuals undergoing physical examinations dur-ing the same period. These individuals were admitted to the Ophthalmology Department of Second Central Hospital of Baoding City, He-bei Province, between September 2022 and September 2023. Retinal ganglion RGC-5 cells were cultured and assigned to the followinggroups: blank group, high glucose group, high glucose + small interfering RNA (siRNA, si) NC group, high glucose + si Circ_001209group, high glucose + si Circ_001209 + miRNA inhibitor NC group, and high glucose+si Circ_001209 + miR-138-5p inhibitor group. MTT assay and flow cytometry were used to detect the proliferation and apoptosis of RGC-5 cells. qRT-PCR was performed to measure the mRNA expression of Circ_001209, miR-138-5p, and SOX7 in RGC-5 cells. Dual-luciferase reporter assay was applied to verify the targeting relationship between miR-138-5p and Circ_001209/SOX7. Western blotting was applied to detect the protein expression of SOX7, B cell-lymphoma-2 (Bcl-2), and Bcl-2 associated X protein (Bax). Results The expression levels of Circ_001209 and SOX7 mRNA in the serum of DR patients were significantly up-regulated, while miR-138-5p expression was markedly down-regulated(P < 0.05). Compared with the blank group, the apoptosis rate [(32.24±3.24) % vs. (7.04±0.71) % ], the expression levels of Circ_001209,SOX7 mRNA and protein, and Bax protein in the high glucose group were significantly increased, while the proliferation rate [(44.01±4.41) % vs. (94.25±5.18) %] and the expression of miR-138-5p and Bcl-2 protein were significantly decreased (P < 0.05). Compared with the high glucose + si NC group, the apoptosis rate [(17.34±1.74) % vs. (32.11±3.22) % ], the expression levels of Circ_001209,SOX7 mRNA and protein, and Bax protein in the high glucose + si Circ_001209 group were significantly decreased, while the prolifera-tion rate [(78.28±7.84) % vs. (43.87±4.39) %], miR-138-5p expression, and Bcl-2 protein expression were significantly increased (P < 0.05). The miR-138-5p inhibitor reversed the protective effect of Circ_001209 knockdown on high-glucose-induced RGC-5 cells. MiR-138-5p exhibited a targeting regulatory relationship with Circ_001209 and SOX7.Conclusions Knockdown of Circ_001209 inhibits high-glucose-induced apoptosis of retinal ganglion cells and alleviates high-glucose-induced damage to RGC-5 cells. The mechanism may involve upregulating the miR-138-5p/SOX7 axis. |
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