| 李波波.司库奇尤单抗治疗银屑病的LncRNA表达差异研究[J].安徽医药,待发表. |
| 司库奇尤单抗治疗银屑病的LncRNA表达差异研究 |
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| 投稿时间：2025-08-16 录用日期：2025-09-17 |
| DOI： |
| 中文关键词: 银屑病 外泌体 lncRNA 司库奇尤单抗 |
| 英文关键词: |
| 基金项目:内蒙古自治区医师协会临床医学研究和临床新技术推广项目(YSXH2024KYF002)；公立医院科研联合基金科技项目(2024GLLH0048)； |
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| 中文摘要: |
| 目的 采用高通量测序结合生物信息学分析方法探索司库奇尤单抗治疗银屑病对血浆外泌体lncRNA表达水平的影响。方法 随机收集2023年9月-2024年5月于内蒙古自治区人民医院皮肤性病科就诊的接受司库奇尤单抗治疗16周的中重度斑块状银屑病患者10名,分别提取这10名患者用药前后血浆中的外泌体,提取RNA进行RNA-seq转录组测序, 通过筛选差异表达的lncRNA和mRNA,预测差异lncRNA的靶基因,并与差异mRNA进行交叉比对,最终对共有靶基因开展GO功能注释及KEGG通路富集分析。结果 本研究检测到差异表达的lncRNA和mRNA,数目分别为37656个和17799个,其中1744个lncRNA和253个mRNA显著上调, 1517个lncRNA和298个mRNA显著下调；GO结果提示,差异lncRNA靶基因可能通过调控染色体结构功能、影响基因稳定性、影响跨膜转运(阴离子和有机酸)等参与银屑病的发展；KEGG结果提示, 差异lncRNA靶基因主要富集在系统性红斑狼疮、中性粒细胞胞外诱捕网(NETs)、嗅觉信号转导通路、Toll样受体通路、趋化因子信号通路。结论 银屑病患者血浆外泌体中差异表达的lncRNA可能与疾病的发病机制密切相关,为司库奇尤单抗治疗银屑病提出基因层面上可能的途径,也为银屑病的临床诊断、治疗及预后评估提供了新的潜在靶点。 |
| 英文摘要: |
| Study on Differential LncRNA Expression in Psoriasis Treated with Secukinumab
Objective This study aims to elucidate the effect of Secukinumab on the psoriasis transcriptome by analyzing longitudinal changes in long non-coding RNA (lncRNA) expression in plasma-derived exosomes from patients undergoing treatment, using high-throughput RNA sequencing and comprehensive bioinformatic analysis.Methods Ten patients with moderate-to-severe psoriasis were prospectively enrolled and received 16-week Secukinumab therapy at the People’s Hospital of Inner Mongolia between September 2023 and May 2024. Plasma-derived extracellular vesicles were isolated from all participants before and after treatment. RNA was extracted and subjected to high-throughput transcriptome sequencing to identify differentially expressed lncRNAs and mRNAs. Potential targets of dysregulated lncRNAs were predicted computationally and validated via overlap analysis with differentially expressed mRNAs. Functional enrichment analyses, including Gene Ontology (GO) and KEGG pathway analyses, were subsequently conducted.Results A total of 37,656 lncRNAs and 17,799 mRNAs were found to be differentially expressed. Among these, 1,744lncRNAs and 253 mRNAs were significantly upregulated, while 1,517 lncRNAs and 298 mRNAs were significantly downregulated. GO analysis indicated that the target genes of differentially expressed lncRNAs may contribute to psoriasis pathogenesis through regulating chromosomal structure and function, maintaining genomic stability, and modulating transmembrane transport of anions and organic acids. KEGG pathway analysis revealed significant enrichment in systemic lupus erythematosus, neutrophil extracellular trap formation, olfactory signaling, Toll-like receptor signaling, and chemokine signaling pathways.Conclusion Differential expression of lncRNAs in plasma-derived exosomes may play a critical role in the pathogenesis of psoriasis, unveiling novel molecular mechanisms behind Secukinumab’s efficacy. These findings provide potential new targets for the clinical diagnosis, treatment, and prognostic assessment of psoriasis. |
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