| 李媛,刘娟,范伟,等.参藤养血颗粒质量标准研究[J].安徽医药,2025,29(9):1758-1762. |
| 参藤养血颗粒质量标准研究 |
| Quality standard of Shenteng Yangxue granules |
| |
| DOI：10.3969/j.issn.1009-6469.2025.09.012 |
| 中文关键词: 中药制剂分析 质量控制 参藤养血颗粒 薄层色谱法 高效液相色谱法 橙皮苷 质量标准 |
| 英文关键词: Analysising drug form of Chinese materia medica Quality control Shenteng Yangxue granules Thin layer chroma-tography High performance liquid chromatography Hesperidin Quality control |
| 基金项目:上海市卫生健康委员会科研项目( 20214Y0280)；上海市浦东新区卫生系统优秀青年医学人才培养计划( PWRq2020-48)；上海中医药大学附属第七人民医院人才培养计划( BDX2022-01，XX2023-11) |
|
| 摘要点击次数: 227 |
| 全文下载次数: 366 |
| 中文摘要: |
| 目的建立参藤养血颗粒的质量标准。方法借助薄层色谱法( TLC)对参藤养血颗粒处方中白术、陈皮、女贞子和制何首乌开展定性鉴别；借助高效液相色谱法( HPLC)对参藤养血颗粒中橙皮苷的含量进行测定。结果白术、陈皮、女贞子和制何首乌薄层色谱鉴别分离度良好，专属性强，且阴性样品无干扰；用 HPLC法测定橙皮苷对照品，其在 0.4~2.0 μg(r=0.999 7)范围内线性关系良好，加样回收率均值为 98.14%(n=6)RSD值为 0.47%，3批参藤养血颗粒中橙皮苷平均含量分别为 0.156 8、 0.154 1、0.156 4 g/L。结论该文建立的 TLC和 HPLC操作简便，专属性强，稳定性及重复性良好，可作为参藤养血颗粒的方法，质量控制方法。 |
| 英文摘要: |
| Objective To establish the quality standard of Shenteng Yangxue granules.Methods Thin layer chromatography (TLC) was used to carry out qualitative identification of Atractylodis Macrocephalae Rhizoma, Citrus reticulata Blanco, Ligustrum lucidum Ait and Polygoni multiflori Radix Praeparata in the prescription of Shenteng Yangxue granules; High performance liquid chromatography (HPLC) was used to determine the content of hesperidin in Shenteng Yangxue granules.Results The TLC of Atractylodis Macrocepha. lae Rhizoma, Citrus reticulata Blanco, Ligustrum lucidum Ait and Polygoni multiflori Radix Praeparata was distinct and highly specific,with no interference of negative samples. The HPLC method was used to determine the hesperidin reference sample. The linear relation-ship was good within the range of 0.4~2.0 μg (r=0.999 7). The mean spiked recovery rate was 98.14% (n=6), with an RSD of 0.47%. The average content of hesperidin in three batches of Shenteng Yangxue granules was 0.156 8, 0.154 1, 0.156 4 g/L, respectively.Con. clusion The methods of TLC and HPLC are simple, specific, stable and reproducible, which can be used for the quality control ofShenteng Yangxue granules. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
