| 罗亚兰,陈清霞.血清外泌体微 RNA-191在胰腺导管腺癌诊断及预后评价中的价值[J].安徽医药,2025,29(9):1762-1766. |
| 血清外泌体微 RNA-191在胰腺导管腺癌诊断及预后评价中的价值 |
| Diagnostic and prognostic value of serum exosomal miR-191 levels in pancreatic ductal adenocarcinoma |
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| DOI：10.3969/j.issn.1009-6469.2025.09.013 |
| 中文关键词: 胰腺肿瘤 微 RNA-191 癌，导管 慢性胰腺炎 糖类抗原 19-9 诊断 预后 |
| 英文关键词: Pancreatic neoplasms MiR-191 Carcinoma, ductal Chronic pancreatitis Carbohydrate antigen 19-9 Diagno-sis Prognosis |
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| 中文摘要: |
| 目的分析胰腺导管腺癌( PDAC)病人血清外泌体微 RNA-191(miR-191)水平变化，并探讨其诊断和预后价值。方法选取 2017年 6月至 2020年 12月成都市第三人民医院收治的 PDAC病人 68例( PDAC组)、慢性胰腺炎( CP)病人 33例( CP组)和医院体检健康者 41例(健康对照组)为研究对象。采用实时荧光定量 PCR法检测血清外泌体 miR-191的表达水平；分析 PDAC组病人血清外泌体 miR-191水平变化；采用受试者操作特征曲线( ROC曲线)评估血清外泌体 miR-191在 PDAC中的诊断价值，并探讨其与 PDAC组病人临床病理特征的关系；采用 Kaplan-Meier生存曲线法和 Log-Rank检验分析血清外泌体 miR-191水平与 PDAC病人预后的关系。结果 PDAC组病人血清外泌体 miR-191表达水平 2.691±0.892显著高于健康对照组 1.001±0.487及 CP组 1.253±0.656(F=30.09，P<0.001)。血清外泌体 miR-191单独诊断 PDAC的 ROC曲线下面积为 0.82，其诊断效能显著优于糖类抗原 19-9(Z=2.50，P=0.013)。PDAC病人血清外泌体 miR-191高表达与临床 TNM分期( χ2=3.84，P=0.049)和淋巴结转移(χ2=4.77，P=0.029)呈显著相关。此外，血清外泌体 miR-191表达水平较高的 PDAC病人总体生存率较低，差异有统计学意义(Log-Rank χ2=11.63，P<0.001)。结论 PDAC病人血清外泌体 miR-191表达水平明显升高，可能有助于 PDAC的临床诊断及预后评估。 |
| 英文摘要: |
| Objective To investigate the changes of serum exosomal MicroRNA 191 (miR-191), as well as its diagnostic and prognos-tic value, in the pancreatic ductal adenocarcinoma (PDAC) patients.Methods A total of 68 PDAC patients and 33 chronic pancreati-tis (CP) patients, who were admitted to The Third People's Hospital of Chengdu and 41 healthy controls undergoing physical examina-tion in the same hospital from June 2017 to December 2020 were enrolled in this study. The levels of serum exosomal miR-191 in these subjects were detected by real-time fluorescence quantitative PCR (RT-qPCR). Changes in serum exosomal miR-191 expression levelswere analyzed in PDAC group. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy of serumexosomal miR-191 in PDAC patients, and the relationship between serum exosomal miR-191 levels and clinicopathological characteris-tics was also explored. Kaplan-Meier survival curves and Log-Rank test were used to analyze the relationship between serum exosomal miR-191 levels and prognosis of PDAC patients.Results The expression level of serum exosomal miR-191 in PDAC group was signifi-cantly higher than that in control and CP groups (2.691±0.892 vs. 1.001±0.487, 1.253±0.656)(F=30.09,P<0.001). The area under the ROC curve of serum exosomal miR-191 for the diagnosis of PDAC was 0.82. The diagnostic efficacy of serum exosomal miR-191 was better than carbohydrate antigen 19-9(Z=2.50,P=0.013). High expression of serum exosomal miR-191 in PDAC patients showed signif-icant correlation with clinical TNM staging (χ2=3.84,P=0.049) and lymph node metastasis (χ2=4.77,P=0.029).Additionally, PDAC pa-tients with high level of serum exosomal miR-191 showed significantly lower overall survival rate with statistical difference (Log-Rank χ2=11.63, P<0.001).Conclusion Increased expression level of serum exosomal miR-191 in PDAC patients may contribute to the clini-cal diagnosis and prognosis assessment of PDAC. |
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