| Objective To analyze the gene expression of mouse intracranial hemorrhage (ICH) tissues based on bioinformatics meth-ods, and to explore the differentially expressed genes (DEGs) affecting ICH development and drug prediction for disease treatment.Methods Gene expression profiles were obtained by downloading GSE216607 and GSE200575 from the Gene Expression Omnibus(GEO) for analysis, and DEGs were screened (P<0.05,|logFC|≥2), which were taken to be intersected. Then the signaling pathways ofthe target genes were enriched using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Next the protein-pro-tein interaction (PPI) network was constructed by Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database. Final-ly, the hub genes were screened in Cytoscape software, and real-time quantitative polymerase chain reaction (RT-qPCR) was performed to validate the hub genes, and the key genes were imported into Coremine Medical database to map out the drugs with potential thera-peutic effects. Transcription factors (TFs) associated with DEGs were predicted using the University of California, Santa Cruz GenomicsInstitute (UCSC) Genome Browser Database in conjunction with the JASPAR database, a database of transcription factor binding profiles.Results A total of 81 up-regulated DEGs were identified, and GO enrichment analysis results showed that they were involved in leuko-cyte migration, cytokine activity and so on. Moreover, KEGG enrichment analysis results showed that they were related to cytokine signal-ing pathway, IL-17 signaling pathway, tumor necrosis factor (TNF) signaling pathway and other signaling pathways. Five hub genes, inter-leukin-1 beta (IL-1β), chemokine (C-C motif) ligand 2 (CCL2), C-X-C motif chemokine ligand 1 (CXCL1), tissue inhibitor of metallopro-teinases 1 (TIMP1), and Serpin family E member 1 (SERPINE1), were finally screened by PPI network. RT-qPCR showed that the mRNA content of IL-1β, CCL2, CXCL1, TIMP1, and SERPINE1 were significantly increased in the brain tissues of mice in the ICH model groupwhen compared with the Sham group (P<0.05). The herbal medicines such as ginseng flower, ginseng lu, ginseng leaf, ginseng, and teatree root, which were screened by Coremine Medical, may serve as potential source of molecular drugs for ICH, and screened moleculessuch as Inermin, Aposiopolamine, Frutinone A, arachidonate, alexandrin_qt, beta-sitosterol, Stigmasterol, Girinimbin, sitosterol, 9,12,15-docosatrienol, icosa-11,14,17-trienoic acid methyl ester as potential molecular drugs for the treatment of ICH.Conclusion Genes such as IL-1β, CCL2, CXCL1, TIMP1, and SERPINE1 are involved in the pathological mechanisms following intracranial hemorrhage, andthat Inermin, Aposiopolamine, Frutinone A, arachidonate, alexandrin_qt, beta-sitosterol, Stigmasterol, Girinimbin, sitosterol, 9, 12, 15-docosatrienol, and icosa-11,14,17-trienoic acid methyl ester may be potential molecular drugs for the treatment of ICH. |
