| 吴金芳,祝哲敏,王歆博,等.根皮苷对盐酸洛哌丁胺所致慢传输型便秘小鼠的通便作用及机制初探[J].安徽医药,2025,29(12):2360-2364. |
| 根皮苷对盐酸洛哌丁胺所致慢传输型便秘小鼠的通便作用及机制初探 |
| Effect of phloridzin on slow-transit constipation in mice induced by loperamide |
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| DOI：10.3969/j.issn.1009-6469.2025.12.006 |
| 中文关键词: 根皮苷 慢传输型便秘 胃肠激素 肠道菌群 钠葡萄糖共转运蛋白 1 |
| 英文关键词: Phloridzin Functional constipation Gastrointestinal hormone Gut microbiota Sodium glucose cotransporter 1 |
| 基金项目: |
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| 中文摘要: |
| 目的基于钠葡萄糖共转运蛋白 1(SGLT1)抑制剂发挥通便作用的多种优势，探究根皮苷对盐酸洛哌丁胺所致慢传输型便秘小鼠的治疗作用，初步阐释根皮苷对慢传输型便秘的治疗机制。方法 2022年 5—11月将 50只雄性 ICR小鼠通过随机数表法分为五组:空白对照组、模型组、低剂量组( 20 mg/kg根皮苷)、高剂量组( 40 mg/kg根皮苷)、阳性组(莫沙必利)，每组各 10只。空白对照组灌胃 0.9%氯化钠溶液，其他四组通过连续灌胃盐酸洛哌丁胺制造慢传输型便秘小鼠模型，模型成立后分别以根皮苷低、高剂量以及莫沙必利连续经口给药治疗 7d。以小鼠体质量和排便行为变化明确其药效，以酶联免疫吸附分析(ELISA)检测血清胃肠激素水平，以阿利新蓝( AB)染色和苏木精 -伊红( HE)染色评价结肠组织的黏液分泌情况及组织结构，以 16S rRNA表征小鼠肠道菌群组成及丰度变化。结果治疗后，根皮苷高剂量组小鼠的首粒黑便时间( 165.63±43.79)min显著低于模型组( 233.13±47.05)min、粪便含水率( 53.69±6.06)%则显著高于模型组( 38.17±6.63)%(P<0.05)。根皮苷同时改善了便秘小鼠血清 5-羟色胺( 5-HT)和血管活性肠肽( VIP)水平的异常变化，其中根皮苷高剂量组小鼠的血清 5-HT(110.78±5.11) μg/L显著高于模型组( 96.38±5.19)μg/L，而与空白对照组( 104.58±6.98)μg/L比较，差异无统计学意义( P>0.05)。此外，根皮苷增加了便秘小鼠的结肠杯状细胞数目并促进其分泌功能，且改变了其肠道菌群组成，增加了有益菌如乳酸杆菌和双歧杆菌的相对丰度。结论根皮苷对盐酸洛哌丁胺所致慢传输型便秘小鼠的治疗作用明确，作用机制可能与血清 5-HT和 VIP功能、结肠黏液分泌以及肠道菌群和微环境的改善有关。 |
| 英文摘要: |
| Objective To investigate the therapeutic effect of phlorizin on loperamide hydrochloride-induced slow transit constipa-tion (STC) in mice and to explain preliminarily the therapeutic mechanism of puerarin on slow transit constipation, based on the multi-ple advantages of sodium-glucose cotransporter 1 (SGLT1) inhibitors in exerting laxative effects. Methods From May to November2022, 50 male ICR mice were divided into 5 groups using a random number table method: blank control group, constipation modelgroup, low-dose group (20 mg/kg phlorizin), high-dose group (40 mg/kg phlorizin), and positive group (mosapride), with 10 mice in eachgroup. During the study, the blank control group was gavaged with 0.9% sodium chloride solution, while the other 4 groups were contin-uously gavaged with loperamide hydrochloride to establish the STC mouse model. After the model was successfully established, themice in each group were orally administered with low-dose phlorizin, high-dose phlorizin, and mosapride respectively for 7 consecutivedays. The efficacy was evaluated based on changes in body weight and defecation behavior of the mice. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum gastrointestinal hormones. Alcian blue (AB) staining and hematoxylin-eosin (HE)staining were performed to assess the mucus secretion and histological structure of the colonic tissue. 16S rRNA sequencing was usedto characterize the composition and abundance changes of the intestinal flora in mice.Results After treatment, the time to first black stool in the high-dose phlorizin group (165.63±43.79) min was significantly shorter than that in the model group (233.13±47.05) min,and the fecal water content (53.69±6.06)% was significantly higher than that in the model group (38.17±6.63)%, with statistically signif-icant differences (P<0.05). Phlorizin also improved the abnormal changes in serum 5-hydroxytryptamine (5-HT) and vasoactive intesti-nal peptide (VIP) levels in constipated mice. Specifically, the serum 5-HT level in the high-dose phlorizin group (110.78±5.11) μg/Lwas significantly higher than that in the constipation model group (96.38±5.19) μg/L, compared with the blank control group (104.58±6.98) μg/L; there was no statistically significant difference (P>0.05). In addition, phlorizin increased the number of colonic goblet cellsand promoted their secretory function in constipated mice, altered the composition of intestinal flora, and increased the relative abun-dance of beneficial bacteria such as Lactobacillus and Bifidobacterium.Conclusion Phlorizin has a definite therapeutic effect on loper-amide hydrochloride-induced STC in mice, and its mechanism may be related to the regulation of serum 5-HT and VIP functions, pro-motion of colonic mucus secretion, and improvement of intestinal flora and microenvironment. |
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