| 何青峰,朱砂.神经元特异性蛋白 3对上皮性卵巢癌细胞恶性生物学进程的影响[J].安徽医药,2026,30(1):124-128. |
| 神经元特异性蛋白 3对上皮性卵巢癌细胞恶性生物学进程的影响 |
| Effect of neuronal-specific septin-3 on malignant biological progression of epithelial ovarian cancer cells |
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| DOI：10.3969/j.issn.1009-6469.2026.01.025 |
| 中文关键词: 卵巢肿瘤 神经元特异性蛋白 3 整合位点家族成员 3α 增殖 β连环蛋白 凋亡 小干扰 RNA |
| 英文关键词: Ovarian neoplasms Neuronal-specific septin 3 Wnt3α Proliferation β-catenin Apoptosis Small interfering RNA |
| 基金项目:四川大学华西第二医院新芽基金项目( KX167) |
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| 中文摘要: |
| 目的研究神经元特异性蛋白 3(SEPTIN3)对上皮性卵巢癌 SKOV3细胞恶性生物学进程的影响。方法研究时间为 2023年 5—11月。通过蛋白质印迹法分析上皮性卵巢癌细胞以及正常卵巢上皮细胞中 SEPTIN3的表达；将上皮性卵巢癌 SKOV3细胞设置为 3个实验组:小干扰 RNA阴性对照( siRNA NC)实验组、小干扰 RNA神经元特异性蛋白 3(siRNA SEPTIN3)实验组与 KYA1797K实验组。采用细胞计数试剂盒( CCK-8)法分析 SKOV3细胞的增殖速度；采用 Transwell实验方法分析 SKOV3细胞的侵袭数量；采用流式细胞术检测 SKOV3细胞的凋亡情况；采用蛋白质印迹法分析整合位点家族成员 3α/β连环蛋白( Wnt3α/β-catenin)通路相关蛋白的表达。结果与正常卵巢上皮细胞比较，上皮性卵巢癌细胞的 SEPTIN3表达上调(IOSE80细胞、 SKOV3细胞、 A2780细胞、 HO-8910细胞的 SEPTIN3表达水平分别为 0.19±0.02比 0.81±0.06、0.85±0.03、0.76± |
| 英文摘要: |
| Objective To investigate the effect of neuronal-specific septin 3 (SEPTIN3) on the malignant biological process of epi-thelial ovarian cancer SKOV3 cells.Methods The study period was from May to November 2023. Western blotting was adopted toquantify SEPTIN3 expression in epithelial ovarian cancer cells and normal ovarian epithelial cells. The cells were allocated random-ly into three groups: small interfering RNA negative control (siRNA NC), small interfering RNA neuronal-specific septin 3 (siRNA SEPTIN3), and KYA1797K groups. SKOV3 cell proliferation was measured via cell counting kit-8 (CCK-8) assay. Transwell assaywas utilized to assess SKOV3 cell invasion. Flow cytometry was performed to analyze SKOV3 apoptosis, and Western blotting wasutilized to examine proteins implicated in the Wingless-type MMTV integration site family 3α/β-catenin (Wnt 3α/β-catenin) signal-ing pathway.Results The level of SEPTIN3 was found to be significantly elevated in epithelial ovarian cancer cells when contrast-ed with normal ovarian epithelial cells; the expression levels of SEPTIN3 in IOSE80 cells, SKOV3 cells, A2780 cells and HO-8910 cells were 0.19±0.02, 0.81±0.06, 0.85±0.03, and 0.76±0.05, respectively. Compared with the siRNA NC group, the propagation rateof SKOV3 cells was diminished in the siRNA SEPTIN3 and KYA1797K groups (P < 0.05). The invasive number of SKOV3 cells in the siRNA SEPTIN3 and KYA1797K groups was significantly decreased (P < 0.05). The apoptosis rate of SKOV3 cells in the siR.NA SEPTIN3 and KYA1797K groups was increased (P < 0.05). The expression levels of Wnt3α and β -catenin of SKOV3 cells in siRNA SEPTIN3 and KYA1797K groups were down-regulated; The expression levels of Wnt3α of SKOV3 cells in the three groupswere 0.77±0.08, 0.21±0.05 and 0.23±0.03;The expression levels of β -catenin of SKOV3 cells in the three groups were 0.75±0.03, 0.25±0.05 and 0.22±0.01.Conclusion SEPTIN3 expression is increased in epithelial ovarian cancer cells. SEPTIN3 inhibition de-creases SKOV3 cell proliferation and invasion while increasing apoptosis, which is mediated by its regulation of the Wnt3α/β -catenin pathway. |
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