| 金唤然,高晶晶,王丽红,等.血清长链非编码 RNA-肺腺癌转移相关转录本 1、微 RNA-143-3p表达水平与骨髓增生异常综合征临床预后的相关性[J].安徽医药,2026,30(1):164-169. |
| 血清长链非编码 RNA-肺腺癌转移相关转录本 1、微 RNA-143-3p表达水平与骨髓增生异常综合征临床预后的相关性 |
| Correlation between serum long non-coding RNA metastasis associated lung adenocarcinom a transcript 1 and microRNA-143-3p expression levels and clinical prognosis of patients with myelodysplastic syndrome |
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| DOI：10.3969/j.issn.1009-6469.2026.01.033 |
| 中文关键词: 骨髓增生异常综合征 肺腺癌转移相关转录本 1 微小 RNA-143-3p 病情严重程度 预后 |
| 英文关键词: Myelodysplastic syndrome Metastasis associated lung adenocarcinom a transcript 1 Micro RNA-143-3p Severity of condition Prognosis |
| 基金项目:河南省卫生健康委员会资助项目( LHGJ202100221) |
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| 中文摘要: |
| 目的探讨血清长链非编码 RNA-肺腺癌转移相关转录本 1(lncRNA-MALAT1)、微 RNA-143-3p(miR-143-3p)表达水平与骨髓增生异常综合征( MDS)病人临床预后的相关性。方法选取 2019年 1月至 2020年 6月在郑州大学第一附属医院诊治的 91例 MDS病人为观察组，另外选取同时期该院体检健康的 53例志愿者作为健康组。采用实时荧光定量 PCR(qRT-PCR)法检测血清 lncRNA-MALAT1、miR-143-3p表达水平；收集分析病人的临床病理特征与血清 lncRNA-MALAT1、miR-143-3p表达水平的关系；使用 ENCORI数据库预测血清 lncRNA-MALAT1与 miR-143-3p的靶向关系； Pearson法分析血清 lncRNA-MALAT1与 miR-143-3p表达的相关性； Spearman法分析血清 lncRNA-MALAT1、miR-143-3p与病情严重程度的相关性；血清 lncRNA-MALAT1和 miR-143-3p水平与预后的关系采用 Kaplan-Meier法分析。结果与健康组相比， MDS病人血清 lncRNA-MALAT1表达水平显著上调( 1.01±0.13比 2.33±0.45)，miR-143-3p表达水平显著下调( 0.99±0.10比 0.52±0.12)(P<0.001)； miR-143-3p与 lncRNA-MALAT1存在靶向结合位点，且病人 lncRNA-MALAT1与 miR-143-3p表达呈负相关( r=.0.70，P<0.001)；分析 MDS病人临床特征发现血清 lncRNA-MALAT1、miR-143-3p表达水平与国际预后评分系统( IPSS-R)分级有关( P<0.001)且随着 MDS病人病情严重程度的增加， lncRNA-MALAT1水平逐渐升高(2.12±0.36比 2.35±0.41比 2.58±0.52)miR-143-3p水平逐，渐降低(0.62± 0.18比 0.50±0.15比 0.41±0.11)(P<0.001)； Spearman分析显示血清 lncRNA-MALAT1与病情严，重程度呈正相关( r=0.52，P< 0.001)miR-143-3p与病情严重程度呈负相关( r=.0.56，P<0.001)；生存分析结果显示 lncRNA-MALAT1低表达 MDS病人 3年生存率( 34/，42，80.95%)高于 lncRNA-MALAT1高表达病人( 28/49，57.14%)(χ2=6.40，P=0.011)； miR-143-3p高表达 MDS病人 3年生存率( 35/44，79.54%)高于 miR-143-3p低表达病人( 27/47，61.70%)(χ2=5.11，P=0.024)。结论 MDS病人血清中 lncRNA-MALAT1表达水平上调， miR-143-3p表达水平下调，二者水平变化与病情严重程度和预后密切相关，有望成为评估 MDS病人预后的靶点。 |
| 英文摘要: |
| Objective To investigate the correlation between the expression levels of serum long non-coding RNA metastasis associat-ed lung adenocarcinom a transcript 1 (lncRNA MALAT1) and microRNA-143-3p (miR-143-3p) with clinical prognosis of myelodysplas-tic syndrome (MDS) patients.Methods Ninety-one MDS patients diagnosed and treated in The First Affiliated Hospital of ZhengzhouUniversity from January 2019 to June 2020 were selected as the observation group, and 53 healthy volunteers who underwent physicalexaminations in the same hospital as the health group. Real time fluorescence quantitative PCR (qRT-PCR) method was applied to de-tect the expression levels of serum lncRNA-MALAT1 and miR-143-3p; the relationship between the clinical and pathological character-istics of patients and the expression levels of serum lncRNA-MALAT1 and miR-143-3p was collected and analyzed; the ENCORI data-base was applied to predict the targeting relationship between serum lncRNA-MALAT1 and miR-143-3p; Pearson method was applied to analyze the correlation between serum lncRNA-MALAT1 and miR-143-3p expression; Spearman method was applied to analyze the correlation between serum lncRNA-MALAT1, miR-143-3p and the severity of disease; the relationship between serum levels of ln-cRNA-MALAT1 and miR-143-3p and prognosis was analyzed using Kaplan-Meier method.Results Compared with the health group, the serum lncRNA-MALAT1 expression level in MDS patients was obviously upregulated (1.01±0.13 vs. 2.33±0.45), and the miR-143-3p expression level was obviously downregulated (0.99±0.10 vs. 0.52±0.12) (P<0.001); miR-143-3p had a targeted binding site with ln-cRNA MALAT1, and there was a negative correlation between the expression of lncRNA MALAT1 and miR-143-3p in patients (r= .0.70, P<0.001); by analyzing the clinical characteristics of MDS patients, it was found that the expression levels of serum lncRNA-MALAT1 and miR-143-3p were related to international prognostic scoring system (IPSS-R) grading (P<0.001), and as the severity of MDS patients increased, the level of lncRNA-MALAT1 gradually increased (2.12±0.36 vs. 2.35±0.41 vs.2.58±0.52), and the level of miR-143-3p gradually decreased (0.62±0.18 vs. 0.50±0.15 vs. 0.41±0.11) (P<0.001); Spearman analysis showed that serum lncRNA-MALAT1 was positively correlated with the severity of disease (r=0.52, P<0.001), while miR-143-3p was negatively correlated with the severity of disease (r=.0.56, P<0.001); the survival analysis results showed that the 3-year survival rate (34/42, 80.95%) of MDS pa-tients with low expression of lncRNA-MALAT1 was higher than that of lncRNA-MALAT1 patients with high expression (28/49, 57.14%) (χ2=6.40, P=0.011); the 3-year survival rate of MDS patients with high expression of miR-143-3p (35/44, 79.54%) was higher than that of patients with low expression of miR-143-3p (27/47, 61.70%) (χ2=5.11, P=0.024).Conclusions The expression level of ln-cRNA-MALAT1 is upregulated and the expression level of miR-143-3p is downregulated in the serum of MDS patients. The changes intheir levels are closely related to the severity and prognosis of disease, and they are expected to become targets for clinical prognosis ofMDS patients. |
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