| 王俊丽,张鼎旋,渠婧,等.他克莫司下调肾病大鼠瞬时受体电位阳离子通道蛋白 6的表达对蛋白尿和肾小球硬化的影响[J].安徽医药,2026,30(2):264-268. |
| 他克莫司下调肾病大鼠瞬时受体电位阳离子通道蛋白 6的表达对蛋白尿和肾小球硬化的影响 |
| Ramification of tacrolimus in proteinuria and glomerulosclerosis by down-regulating the expression of TRPC6 in nephrotic rats |
| |
| DOI：10.3969/j.issn.1009-6469.2026.02.010 |
| 中文关键词: 他克莫司 肾病 阿霉素 肾小球硬化 瞬时受体电位阳离子通道蛋白 6 |
| 英文关键词: Tacrolimus Nephrosis Adriamycin Glomerulosclerosis TRPC6 |
| 基金项目:国家重点研发计划项目( 2022YFC2705100)；广州市科技计划项目( 2024A03J1018)；广州市卫生计生科技一般引导项目( 20221A011009、20241A011020) |
|
| 摘要点击次数: 420 |
| 全文下载次数: 205 |
| 中文摘要: |
| 目的探讨瞬时受体电位阳离子通道蛋白 6(TRPC6)在蛋白尿发病机制中的作用，以及他克莫司治疗肾小球硬化的作用机制。方法 2024年 1―7月， 30只健康雄性 SD大鼠采用随机数字表法分成健康组、阿霉素肾病模型组和他克莫司治疗组，各 10只，常规检测各组大鼠 24 h尿蛋白定量及血生化指标，应用免疫组化技术检测足细胞 TRPC6的分布和表达。结果与健康组比较，阿霉素肾病模型组大鼠 24 h尿蛋白显著增加［(342.17±13.70)mg/24 h比( 12.12±3.91)mg/24 h］并出现低蛋白血症、高脂血症、肾功能损害和 FSGS病理变化，同时肾小球和肾小管间质中 TRPC6蛋白表达量明显升高( 543.5，8±56.07比 166.37±32.53)；与阿霉素肾病模型组大鼠比较，他克莫司治疗组大鼠 24 h尿蛋白( 88.58±24.12)mg/24 h显著减少及病理改变减轻，同时进一步降低了 TRPC6的表达( 312.63±38.24)从而减轻了蛋白尿和改善肾小球硬化。结论 TRPC6在阿霉素肾病大鼠肾小球的表达明显升高，表明 TRPC6是肾小球足细胞损，伤的指标之一，而他克莫司可能通过下调肾病大鼠 TRPC6的表达对蛋白尿和肾小球硬化损害起到治疗作用。 |
| 英文摘要: |
| Objective The role of TRPC6 in the pathogenesis of proteinuria and the molecular mechanism of tacrolimus in the treat-ment of glomerulosclerosis were studied.Methods From January to July 2024, 30 healthy male SD rats were randomly assigned to oneof three groups: normal control group, adriamycin nephropathy model group and tacrolimus treatment group, with 10 rats in each catego-ry. The 24 h urinary protein and blood biochemical indexes of rats in each group were routinely detected. The presence and expressionof TRPC6 in podocytes were assessed using immunohistochemical techniques.Results Compared with the normal control group, the 24-hour urinary protein of rats in the adriamycin nephropathy model group was significantly increased[(342.17±13.70) mg/24 h vs. (12.12±3.91) mg/24 h], accompanied by hypoproteinemia, hyperlipidemia and renal pathological changes of FSGS. At the same time,the expression of TRPC6 protein (543.58±56.07 vs. 166.37±32.53) in glomeruli and tubulointerstitium was significantly increased.Compared with the adriamycin nephropathy model group, the 24 h urinary protein (88.58±24.12) mg/24 h of rats treated with tacrolimuswas significantly reduced, and the expression of TRPC6 (312.63±38.24) was further reduced, thereby alleviating proteinuria and glo-merulosclerosis.Conclusion The expression of TRPC6 in glomeruli of adriamycin nephropathy rats was significantly increased, indi-cating that TRPC6 is one of the indexes of glomerular podocyte injury, and tacrolimus plays a therapeutic role in proteinuria and glo-merulosclerosis by down-regulating the expression of TRPC6 in nephrotic rats. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
