| 毕正,经加吻,朱晋如,等.基于 UPLC-QExactive-Orbitrap-MS的丹蛭降糖胶囊化学成分分析[J].安徽医药,2026,30(8):1550-1555. |
| 基于 UPLC-QExactive-Orbitrap-MS的丹蛭降糖胶囊化学成分分析 |
| Analysis of the chemical composition of Danzhi Jiangtang capsules based on UPLC-QExactive-Orbitrap-MS |
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| DOI：10.3969/j.issn.1009-6469.2026.08.013 |
| 中文关键词: 主成分分析 丹蛭降糖胶囊 液质联用 化学成分 中草药 复方合剂 化学，分析 超高效液相色谱 -四极杆 -静电场轨道阱高分辨质谱 |
| 英文关键词: Principal component analysis Danzhi Jiangtang capsules Liquid mass spectrometry Chemical composition Drugs, Chinese herbal Drug combinations Chemistry, analytic UPLC-QExactive-Orbitrap-MS |
| 基金项目:国家自然科学基金项目( 82174153)；安徽省高校协同创新项目( GXXT-2020-025)； 2型糖尿病中医药循证能力提升项目( 2023-24)；合肥综合性国家科学中心大健康研究院“揭榜挂帅”项目( 2023CXMMTCM003) |
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| 中文摘要: |
| 目的运用超高效液相色谱 -四极杆 -静电场轨道阱高分辨质谱( UPLC-QExactive-Orbitrap-MS)技术对丹蛭降糖胶囊 |
| 英文摘要: |
| Objective To conduct a systematic analysis of the main chemical components of Danzhi Jiangtang capsules (DJC) using Ultra-high performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry (UPLC-QExac-tive-Orbitrap-MS), so as to provide a scientific basis for further research on its clinical efficacy and mechanism of action. Methods From April to June 2024, UPLC-QExactive-Orbitrap-MS was used to conduct in-depth analysis of the chemical components of DJC.The samples were ultrasonically extracted with 60% methanol, then separated by the UPLC system, and finally identified using high-resolution mass spectrometry.Results This study successfully identified 164 chemical components in DJC, including flavonoids, ter-penoids, phenylpropanoids, saccharides, phenolic acids, alkaloids, cyclic peptides, organic acids, and other types of compounds.Among them, 22 compounds were derived from Moutan Cortex, 21 from Pseudostellaria heterophylla, 19 from Hirudo, 15 from SemenCuscutae, 16 from alisma, and 6 from rehmannia.Conclusions Using UPLC-QExactive-Orbitrap-MS technology, this study has com-prehensively revealed the complex chemical composition of DJC. The wide presence of the components validates its diverse biologicalactivities, providing a solid scientific foundation for further research and clinical application of DJC. |
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