| 李芹,胡彬,鲁圆圆,等.微 RNA-532-5p、微 RNA-200c在上皮性卵巢癌组织中的表达及其与增殖侵袭基因、预后的关系[J].安徽医药,2026,30(8):1555-1560. |
| 微 RNA-532-5p、微 RNA-200c在上皮性卵巢癌组织中的表达及其与增殖侵袭基因、预后的关系 |
| The expression of miR-532-5p and miR-200c in epithelial ovarian cancer and its relationship with proliferation and invasion genes and prognosis |
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| DOI：10.3969/j.issn.1009-6469.2026.08.014 |
| 中文关键词: 卵巢肿瘤 微 RNA-532-5p 微 RNA-200c 增殖基因 侵袭基因 预后 |
| 英文关键词: Ovarian neoplasms MiR-532-5p MiR-200c Proliferating gene Invasion gene Prognosis |
| 基金项目:云南省卫生健康委临床医学中心 2020—2022年建设项目( 202012AF013129) |
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| 中文摘要: |
| 目的探讨微 RNA(miR)-532-5p、miR-200c在上皮性卵巢癌( EOC)组织中的表达与增殖侵袭基因及预后的关系。方前瞻性选取 2020年 1月至 2021年 5月昆明市延安医院 EOC病人 120例作为恶性组，另选取同期良性卵巢肿瘤 60例病人作法为良性组。比较两组病灶组织中 miR-532-5p、miR-200c、增殖基因［ FUN14结构域蛋白 1(FUNDC1)、蛋白激酶 B(Akt)、肿瘤坏死因子 α诱导蛋白 8(TNFAIP8)、乙型肝炎病毒 X蛋白结合蛋白( HBXIP)］、侵袭基因［自噬相关基因 Beclin1、沉默信息调节因子 2(SIRT2)、含表皮生长因子的纤维蛋白细胞外基质蛋白 1(EFEMP1)、乳腺癌转移抑制基因( BRMS1)］表达水平，分析 miR-532-5p、miR-200c与增殖侵袭基因的相关性。对恶性组随访 3年，根据生存状况分为生存亚组与死亡亚组，比较两亚组病灶组织中 miR-532-5p、miR-200c表达水平，分析 miR-532-5p、miR-200c与预后的关系。结果恶性组病灶组织中 miR-532-5p表达水平 0.27±0.07低于良性组 1.05±0.11，miR-200c表达水平 1.08±0.20高于良性组 0.71±0.13(P<0.05)；恶性组病灶组织中 FUNDC1、Akt、TNFAIP8、HBXIP、EFEMP1 mRNA相对表达量高于良性组， Beclin1、SIRT2、BRMS1 mRNA相对表达量低于良性组( P<0.05)； miR-532-5p与 FUNDC1、Akt、TNFAIP8、HBXIP、EFEMP1呈负相关，与 Beclin1、SIRT2、BRMS1呈正相关， miR-200c与 FUNDC1、Akt、TNFAIP8、HBXIP、EFEMP1呈正相关，与 Beclin1、SIRT2、BRMS1呈负相关( P<0.05)。随访 3年，死亡亚组 miR-532-5p表达水平 0.19±0.05低于生存亚组 0.29±0.07，miR-200c表达水平 1.18±0.12高于生存亚组 1.06±0.08(P<0.05)；受试者操作特征曲线( ROC曲线)分析， miR-532-5p在生存病人与死亡病人中的最佳截断值为 0.23，此时的曲线下面积( AUC)为 0.81； miR-200c在生存病人与死亡病人中的最佳截断值为 1.11，此时的 AUC为 0.80；KM曲线分析， miR-532-5p低表达病人 3年生存率 62.79%(27/43)低于高表达病人 94.67%(71/75)，miR-200c高表达病人 3年生存率 56.76%(21/37)低于低表达病人 95.06%(77/81)(P<0.05)。结论 EOC组织中 miR-532-5p呈低表达， miR-200c呈高表达，均与增殖侵袭基因表达显著相关，且 miR-532-5p低表达和 miR-200c高表达时会显著降低 3年生存率。 |
| 英文摘要: |
| Objective To investigate the relationship between the expression of micrornA-532-5p (miR-532-5p) and micrornA-200C (miR-200c) in epithelial ovarian cancer (EOC) tissues, proliferation and invasion genes and prognosis.Methods Prospective selectionof 120 patients with EOC from January 2020 to May 2021 in Kunming Yan'an Hospital were selected as the malignant group, and 60patients with benign ovarian tumor during the same period were selected as the benign group. The expression levels of miR-532-5p, miR-200c, proliferating genes [FUN14 domain protein 1 (FUNDC1), protein kinase B (Akt), tumor necrosis factor α-inducing protein 8 (TNFAIP8), hepatitis B virus X-protein binding protein (HBXIP)] and invasive genes [autophagy related genes (Beclin1), silence-infor-mation regulator 2 (SIRT2), EGF containing fibulin extracellular matrix protein 1 (EFEMP1) and breast cancer metastasis suppressorgene (BRMS1)] in the lesion tissues of the two groups were compared. The correlation between miR-532-5p and miR-200c and prolifera-tion and invasion genes was analyzed. The malignant group was followed up for 3 years and divided into survival subgroup and deathsubgroup according to survival status. The expression levels of miR-532-5p and miR-200c in lesion tissues of the two subgroups were compared, and the relationship between miR-532-5p and miR-200c and prognosis was analyzed.Results The expression level of miR-532-5p in malignant group was lower than that in benign group (0.27±0.07 vs. 1.05±0.11), and the expression level of miR-200c was higher than that in benign group (1.08±0.20 vs. 0.71±0.13) (P<0.05). The mRNA relative expressions of FUNDC1, Akt, TNFAIP8,HBXIP and EFEMP1 in malignant group were higher than those in benign group, while the mRNA relative expressions of Beclin1,SIRT2 and BRMS1 in malignant group were lower than those in benign group (P<0.05). MiR-532-5p was negatively correlated withFUNDC1, Akt, TNFAIP8, HBXIP and EFEMP1, and positively correlated with Beclin1, SIRT2 and BRMS1.miR-200c was positivelycorrelated with FUNDC1, Akt, TNFAIP8, HBXIP and EFEMP1, and negatively correlated with Beclin1, SIRT2 and BRMS1 (P<0.05). After 3 years of follow-up, the expression level of miR-532-5p in the death subgroup was lower than that in the survival subgroup(0.19±0.05 vs. 0.29±0.07), and the expression level of miR-200c was higher than that in the survival subgroup (1.18± 0.12 vs. 1.06±0.08) (P< 0.05). The receiver operating characteristic curve (ROC curve) analysis showed that the optimal cut-off value of miR-532-5p in survival patients and death patients was 0.23, and the area under the curve (AUC) was 0.81. The optimal cut-off value of miR-200c in survival patients and death patients was 1.11, and the AUC was 0.80. KM curve analysis showed that the 3-year survival rate of patients with low expression of miR-532-5p was 62.79% (27/43), lower than that of patients with high expression of miR-532-5p which was 94.67% (71/75). The 3-year survival rate of patients with high miR-200c expression was 56.76% (21/37), lower than that of patients with low miR-200c expression which was 95.06% (77/81) (P<0.05). Conclusion The low expression of miR-532-5p and high expression of miR-200c in EOC tissues are significantly correlated with the expression of proliferative and invasive genes, and the low expression of miR-532-5p and high expression of miR-200c will significantly reduce the 3-year survival rate. |
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