| 张翊,李鑫,冉静,等.微 RNA-124-3p、成纤维细胞生长因子受体样因子 1在食管癌组织中的表达与临床病理参数及术后复发转移的相关性[J].安徽医药,2026,30(9):1842-1847. |
| 微 RNA-124-3p、成纤维细胞生长因子受体样因子 1在食管癌组织中的表达与临床病理参数及术后复发转移的相关性 |
| Expression of microRNA-124-3p and fibroblast growth factor receptor-like 1 in esophageal cancer tissues and their association with clinicopathological parameters and postoperative recurrence and metastasis |
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| DOI：10.3969/j.issn.1009-6469.2026.09.027 |
| 中文关键词: 食管肿瘤 临床病理参数 复发 转移 微 RNA-124-3p 成纤维细胞生长因子受体样因子 1 |
| 英文关键词: Esophageal neoplasms Clinicopathological parameters Recurrence Metastasis MicroRNA-124-3p Fibroblast growth factor receptor like 1 |
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| 中文摘要: |
| 目的探究微 RNA(miR)-124-3p、成纤维细胞生长因子受体样因子 1(FGFRL1)在食管癌组织中的表达水平及其与食管癌病人临床病理参数及术后复发转移的相关性。方法前瞻性研究。采用连续入组的方式，选取 2017年 1月至 2021年 12月在重庆市两江新区人民医院接受食管癌根治术的 115例食管癌病人(食管腺癌 20例，鳞癌 95例)为研究对象，根据术后随访有无发生复发转移分为预后良好组( 71例)和预后不良组( 44例)。利用实时荧光定量逆转录聚合酶链反应( qRT-PCR)法检测术中切除食管癌组织和癌旁组织中 miR-124-3p、FGFRL1 mRNA的表达水平，收集食管癌病人的临床病理资料。采用 Pearson相关性分析检验 miR-124-3p与 FGFRL1 mRNA表达之间的相关性；采用 Cox回归分析探究影响食管癌病人术后复发转移的重要因素；采用受试者操作特征曲线( ROC曲线)分析 miR-124-3p与 FGFRL1 mRNA预测食管癌病人术后复发转移的预后价值。结果食管腺癌和鳞癌组织中 miR-124-3p表达水平低于腺癌和鳞癌癌旁组织( 0.76±0.12比 1.04±0.20，0.80±0.13比 1.01±0.17)， FGFRL1 mRNA表达水平高于腺癌和鳞癌癌旁组织( 1.72±0.32比 0.98±0.14，1.77±0.36比 1.02±0.18)(均 P<0.05)。 miR-124-3p与 FGFRL1 mRNA表达之间呈负相关( P<0.05)。 miR-124-3p在低分化、 TNM分期为 Ⅲ期、肿瘤长径 >4 cm、浸润深度为 T3~T4、淋巴结转移 N1~N3的食管癌组织中表达最低， FGFRL1 mRNA在低分化、 TNM分期为 Ⅲ期、肿瘤长径 >4 cm、浸润深度为 T3~T4、淋巴结转移 N1~N3的食管癌组织中表达最高( P<0.05)。预后不良组 TNM分期为 Ⅲ期、淋巴结转移 N1~N3病人占比以及 FGFRL1 mRNA表达( 2.06±0.45比 1.57±0.31)较高， miR-124-3p表达较低( 0.69±0.09比 0.85±0.12)(均 P<0.05)。 TNM分期增加、淋巴结转移、 miR-124-3p表达下降以及 FGFRL1 mRNA表达上升是食管癌病人术后复发转移的危险因素( P<0.05)。联合 miR-124-3p与 FGFRL1 mRNA预测食管癌病人术后复发转移的曲线下面积( AUC)高于单独预测 AUC(P<0.05)。结论食管癌病人癌组织中 miR-124-3p表达下降， FGFRL1 mRNA表达上升，且与病人肿瘤分化程度、 TNM分期、肿瘤长径、浸润深度、淋巴结转移有关；联合 miR-124-3p与 FGFRL1 mRNA可作为术后复发转移的预测标志物。 |
| 英文摘要: |
| Objective To investigate the expression levels of microRNA (miR) -124-3p and fibroblast growth factor receptor like 1(FGFRL1) in esophageal cancer tissues and to analyze their association with clinicopathological parameters and postoperative recur.rence and metastasis in patients with esophageal cancer.Methods This prospective study enrolled 115 patients who underwent radi.cal resection for esophageal cancer at Chongqing Liangjiang New Area People's Hospital from January 2017 to December 2021, includ.ing 20 cases of esophageal adenocarcinoma and 95 cases of esophageal squamous cell carcinoma. The patients were divided into a goodprognosis group (71 cases) and a poor prognosis group (44 cases) based on the presence or absence of recurrence or metastasis duringpostoperative follow-up. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the ex. pression levels of miR-124-3p and FGFRL1 mRNA in surgically resected esophageal cancer tissues and paired adjacent non-cancerous tissues. Clinicopathological data were collected. Pearson correlation analysis was used to assess the correlation between miR-124-3pand FGFRL1 mRNA. Cox regression analysis was performed to identify factors affecting postoperative recurrence and metastasis inesophageal cancer patients. Receiver operating characteristic curve (ROC curve) analysis was used to evaluate the predictive value ofmiR-124-3p and FGFRL1 mRNA for postoperative recurrence and metastasis.Results The expression levels of miR-124-3p in esoph. ageal adenocarcinoma and squamous cell carcinoma tissues was significantly lower than those in adjacent non-cancerous tissues (0.76±0.12 vs. 1.04±0.20, 0.80±0.13 vs. 1.01±0.17), while the FGFRL1 mRNA levels were significantly higher (1.72±0.32 vs. 0.98±0.14, 1.77±0.36 vs. 1.02±0.18) (all P<0.05). A negative correlation was observed between miR-124-3p and FGFRL1 mRNA (P<0.05). The lowest expression of miR-124-3p and the highest expression of FGFRL1 mRNA were found in esophageal cancer tissues with poor dif.ferentiation, TNM stage Ⅲ , tumor diameter >4 cm, depth of invasion T3-T4, and lymph node metastasis N1-N3 (P<0.05). The poor prognosis group had significantly higher proportions of patients with TNM stage Ⅲ and N1-N3 lymph node metastasis, higher FGFRL1 mRNA expression (2.06±0.45 vs. 1.57±0.31), and lower miR-124-3p expression (0.69±0.09 vs. 0.85±0.12) (all P<0.05). Increased TNM stage, lymph node metastasis, decreased expression of miR-124-3p, and increased expression of FGFRL1 mRNA were risk factors for postoperative recurrence and metastasis (P<0.05). The area under the curve (AUC) for the combined use of miR-124-3p and FGFRL1 mRNA in predicting postoperative recurrence and metastasis was significantly higher than that of either marker alone (P<0.05).Conclu. sions The expression of miR-124-3p is downregulated and FGFRL1 mRNA is upregulated in esophageal cancer tissues, and thesechanges are associated with tumor differentiation, TNM staging, tumor size, depth of invasion, and lymph node metastasis. The combina.tion of miR-124-3p and FGFRL1 mRNA may serve as predictive marker for postoperative recurrence and metastasis. |
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