| 林峰旭,邓利超,伟勒斯,等.HMGB-1、p-AKT和 MMP-9在 AGA大鼠关节软骨和滑膜中的表达及意义[J].安徽医药,2026,30(10):1981-1985. |
| HMGB-1、p-AKT和 MMP-9在 AGA大鼠关节软骨和滑膜中的表达及意义 |
| Expression and significance of HMGB-1, p-AKT and MMP-9 inarticular cartilage and synovium of rat with AGA |
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| DOI：10.3969/j.issn.1009-6469.2026.10.014 |
| 中文关键词: 关节炎，痛风性 高迁移率族蛋白 1(HMGB-1) 磷酸化蛋白激酶 B(p-AKT) 基质金属蛋白酶 -9(MMP-9) 超声(US) |
| 英文关键词: Arthritis,gouty High mobility group box1 protein (HMGB-1) Phosphorylated protein kinase B (p-AKT) Matrix me-talloproteinase-9 (MMP-9) Ultrasound(US) |
| 基金项目:内蒙古自治区科技计划项目( 2025YFSH0051) |
| 作者 | 单位 | E-mail | | 林峰旭 | 内蒙古医科大学内蒙古临床医学院,内蒙古自治区呼和,浩特 010017
内蒙古自治区人民医院骨科中心,内蒙古自治区呼和浩特 010017 | | | 邓利超 | 内蒙古自治区人民医院骨科中心,内蒙古自治区呼和浩特 010017 | | | 伟勒斯 | 内蒙古医科大学内蒙古临床医学院,内蒙古自治区呼和,浩特 010017
内蒙古自治区人民医院骨科中心,内蒙古自治区呼和浩特 010017 | | | 闫超 | 内蒙古医科大学内蒙古临床医学院,内蒙古自治区呼和,浩特 010017
内蒙古自治区人民医院骨科中心,内蒙古自治区呼和浩特 010017 | | | 石竟成 | 内蒙古医科大学内蒙古临床医学院,内蒙古自治区呼和,浩特 010017
内蒙古自治区人民医院骨科中心,内蒙古自治区呼和浩特 010017 | | | 王长海 | 内蒙古医科大学内蒙古临床医学院,内蒙古自治区呼和,浩特 010017
内蒙古自治区人民医院骨科中心,内蒙古自治区呼和浩特 010017 | wangchanghai.2008@163.com |
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| 中文摘要: |
| 目的探讨高迁移率族蛋白 B1(HMGB-1)、磷酸化蛋白激酶 B(p-AKT)和基质金属蛋白酶 -9(MMP-9)在急性痛风性关节炎( AGA)大鼠滑膜和关节软骨表达及意义。方法研究起止时间为 2024年 6—12月。将 30只清洁级雄性 Sprague-Dawley(SD)大鼠分为实验组和对照组，每组 15只。两组均在自由摄取诱导饲料( 3%氧嗪酸钾 +15%酵母 +82%基础饲料)和饮用正常水的基础上，连续饲养 6周。实验组将单钠尿酸盐( MSU)结晶混悬液 0.2 mL(25 g/L)经超声( US)介导注射至大鼠右侧踝关节间隙，对照组以相同方法注射 0.2 mL 0.9%氯化钠溶液。造模期间，定时测量大鼠血尿酸值、步态变化，进行肾脏组织病理切片检查，并对滑膜与关节软骨进行组织病理学观察与免疫组织化学染色，评估 HMGB-1、p-AKT和 MMP-9的表达水平。结果 对照组相比，实验组 SD大鼠踝关节肿胀度更高( P<0.05)步态跛行变化更明显( Z=.2.89，P<0.01)。免疫组织化学染色显示，与造模后 48 h实验组滑膜 HMGB-1表达水平为 0.266±0.025，，高于对照组的 0.158±0.008，差异有统计学意义( P<0.01)；各时间点 p-AKT和 MMP-9的表达水平亦均高于对照组( P<0.01)。结论 HMGB-1、p-AKT和 MMP-9在 AGA大鼠滑膜及关节软骨表达水平更高，可能与其参与炎症反应及介导免疫因子的释放有关。 |
| 英文摘要: |
| Objective To investigate the expression and significance of high mobility group box1protein(HMGB-1), phosphorylated protein kinase B (p-AKT), and matrix metalloproteinase-9 (MMP-9) in synovium and articular cartilage of rats with acute gouty arthritis (AGA).Methods The study period was from June 2024 to December 2024. Clean grade male Sprague Dawley (SD) rats were randomlydivided into an experimental group and a control group, 15 rats in each group. Both groups were fed continuously for 6 weeks on the ba-sis of free intake of induction feed (3% potassium oxonate+15% yeast+82% basic feed) and drinking normal water. The experimental group injected 0.2 mL (25 g/L) of monosodium urate (MSU) crystal suspension into the right ankle joint space of rats under ultrasound(US) guidance, while the control group was injected with 0.2 mL of 0.9% sodium chloride solution using the same method. During themodeling period, blood uric acid levels and gait changes in rats were measured at regular intervals, and renal tissue pathology sectionswere examined. Synovium and articular cartilage were observed histologically and stained with immunohistochemistry to evaluate theexpression levels of HMGB-1, p-AKT, and MMP-9. Results Compared with the control group, the experimental group of SD rats showed higher ankle joint swelling (P<0.05) and more significant changes in gait claudication (Z=.2.89, P<0.01). The immunohisto-chemical results showed that the expression level of HMGB-1 in the synovium of the experimental group was 0.266 ± 0.025 at 48 hoursafter modeling, which was higher than that of the control group 0.158 ± 0.008, and the difference was statistically significant (P<0.01). The expression levels of p-AKT and MMP-9 at each time point were also higher than those in the control group (P<0.01).Conclusion HMGB-1, p-AKT, and MMP-9 are expressed at higher levels in the synovium and articular cartilage of SD rats with AGA, which may berelated to their involvement in inflammatory response and mediating the release of immune factors. |
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