| 李凯鑫,马洪,张中严,等.性成熟前期大鼠卵巢扭转对性成熟期健侧卵巢组织结构及相关激素的影响[J].安徽医药,2026,30(10):2061-2066. |
| 性成熟前期大鼠卵巢扭转对性成熟期健侧卵巢组织结构及相关激素的影响 |
| Influences of unilateral ovarian torsion in sex immature on histological changes of contralateral ovarian tissue structure in sex mature rats and the detection of related hormones levels in serum |
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| DOI：10.3969/j.issn.1009-6469.2026.10.029 |
| 中文关键词: 卵巢扭转 卵巢切除术 SD大鼠 形态学 卵泡刺激素 黄体生成素 抗米勒管激素 |
| 英文关键词: Ovarian torsion Ovariectomy SD rats Histomorphology Follicle stimulating hormone Luteinizing hormone Anti-Müllerian hormone |
| 基金项目:遵义市科技计划项目(遵市科合 HZ字〔 2021〕23号) |
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| 中文摘要: |
| 目的探讨性成熟前期 SD大鼠在发生卵巢扭转后，针对扭转卵巢的不同病理状态(包括扭转未坏死、扭转疑似坏死以及扭转坏死)采取什么手术方式(保留 /切除)进行处理，能最大限度地减少性成熟时期的健康侧卵巢生殖功能损害。方法在 2022年 10月至，2024年 1月，参考国际构建“睾丸扭转”动物模型被广泛应用的 Turner法的技术路线，建立卵巢扭转的动物模型。采用完全随机分组法将 56只性成熟前期鼠龄为 3周龄 SD雌鼠，分为七组，每组大鼠数量均为 8只，简单随机抽样选择其中 1组作为假手术对照组( CG组)并进行假手术，剩下的 6组分别制作:扭转未坏死卵巢复位组( NNOR)/切除组( NNOO)、扭转疑似坏死卵巢复位组( SNOR)/切除组( SNOO)、扭转坏死卵巢复位组( NOR)/切除组( NOO)；然后继续喂养大鼠到 8周龄，即达到大鼠性成熟期。采用颈椎脱臼法处死动物，切取健康侧的卵巢石蜡包埋，并进行 HE染色观察组织形态学变化，并收集心脏血液，经离心处理后获取血清，并采用酶联免疫吸附分析( ELISA)检测卵泡刺激素( FSH)、黄体生成素( LH)、抗米勒管激素(AMH)等相关激素水平。结果性成熟期健侧卵巢组织结构改变: CG组健侧卵巢结构较清晰，卵巢的各级卵泡发育良好，成熟卵泡中颗粒细胞层多且清晰、卵泡腔内卵泡液丰富，可见完整的卵母细胞，卵巢间质中未见明显炎症细胞浸润及纤维化； NNOR组/NNOO组:健侧卵巢均未出现明显组织形态学改变； SNOR组/SNOO组:健侧卵巢部分结构排列欠佳，各级卵泡及成熟卵泡数目减少，闭锁卵泡数增多，颗粒细胞排列欠佳。 NOR组/NOO组:前者健侧卵巢结构排列紊乱，各级卵泡数明显减少，闭锁卵泡数明显增多，颗粒细胞层减少、排列紊乱，卵巢间质内可见血管扩张、淋巴细胞浸润；后者，健侧卵巢仅表现为部分卵巢结构排列欠佳。各激素( FSH、LH、AMH)水平在 NNOR组与 NNOO组间比较，以及在 SNOR组与 SNOO组间比较，均差异无统计学意义( Ρ>0.05)；与 NOO组间比较， NOR组 AMH水平［(5 365.69±586.64)ng/L比( 8 712.47±497.31)ng/L］降低， FSH(588.46±55.55比 431.23±36.32)、 LH［( 201.77±16.90)IU/L比( 128.11±12.27)IU/L］水平升高( Ρ<0.05)； CG组中各激素的水平与 NNOR组、 NNOO组以及 NOO组间比较，差异无统计学意义；与 SNOR组、 SNOO组及 NOR组间比较， AMH水平较 CG组低， FSH、LH水平较 CG组高( Ρ<0.05)。结论性成熟前期扭转疑似坏死卵巢具有保留价值，扭转坏死卵巢切除后对性成熟期健侧卵巢具有保护作用。 |
| 英文摘要: |
| Objective To explore what surgical approach (preservation/resection) should be taken for different pathological states ofthe twisted ovary (including torsion without necrosis, suspected torsion necrosis, and torsion necrosis) in pre-pubertal SD rats after ovar-ian torsion to minimize the damage to the reproductive function of the healthy contralateral ovary during sexual maturity. Methods The ovarian torsion animal model was established using a technique analogous to that described by Turner from October 2022 to Janu-ary 2024. Fifty-six female SD rats at the pre -sexual maturity stage with an age of 3 weeks were divided into 7 groups of 8 rats each us-ing a completely random grouping method. And one group was randomly selected to perform the simulated surgery as a control group(CG). The remaining 6 groups were respectively prepared as follows: the non -necrotic ovarian torsion reduction group (NNOR)/the non -necrotic ovarian torsion resection group (NNOO), the suspected necrotic ovarian torsion reduction group (SNOR)/the suspected necrot-ic ovarian torsion resection group (SNOO), the necrotic ovarian torsion reduction group (NOR)/the necrotic ovarian torsion resectiongroup (NOO).. Then continue to raise them until they reach 8 weeks of age, which was the period of sexual maturity. The animals weresacrificed by cervical dislocation method, and the healthy ovary was cut and paraffin-embedded for HE staining to observe the histologi-cal changes. The heart blood was retained and the serum was collected by centrifugation and the levels of related hormones that Folliclestimulating hormone (FSH), Luteinizing hormone (LH), Anti-Müllerian hormone (AMH) were detected by ELISA.Results Changes inthe structure of the healthy ovary during the period of sexual maturity: In the CG group, the structure of the healthy ovary was clear,with good development of follicles at all levels, a clear granulosa cell layer in mature follicles, abundant follicular fluid, and intact oo-cytes visible. There was no obvious inflammatory cell infiltration or fibrosis in the ovarian stroma. In the NNOR group and NNOOgroup: no obvious morphological changes in the healthy ovary were observed. In the SNOR group and SNOO group: some structural ar-rangements in the healthy ovary were poor, with a reduction in the number of follicles at all levels and mature follicles, an increase inthe number of atretic follicles, and poor arrangement of granulosa cells. In the NOR group and NOO group: the former showed disor-dered arrangement of the healthy ovary structure, a significant reduction in the number of follicles at all levels, a significant increase inthe number of atretic follicles, a reduction and disordered arrangement of the granulosa cell layer, and visible vascular dilation and lym-phocytic infiltration in the ovarian stroma. The latter showed only partial poor arrangement of some ovarian structures in the healthy ova-ry. The levels of hormones (FSH, LH, AMH) between the NNOR group and the NNOO group, as well as between the SNOR group andthe SNOO group, showed no statistically significant differences (P > 0.05); when comparing the NOR group with the NOO group, the AMH level [(5 365.69±586.64) ng/L vs. (8 712.47±497.31) ng/L] was decreased and the FSH (588.46±55.55 vs. 431.23±36.32) and LH [(201.77±16.90) IU/L vs. (128.11±12.27) IU/L] levels were increased in the NOR group (P < 0.05); when comparing the hormone levelsin the CG group with those in the NNOR group, NNOO group, and NOO group, there were no statistically significant differences; howev-er, when compared with the SNOR group, SNOO group, and NOR group, the AMH level was lower and the FSH and LH levels werehigher in the CG group (P<0.05).Conclusion Torsion of the suspected necrotic ovary in the prematurity period has preservation val-ue, and the resection of the necrotic ovary has a protective effect on the healthy ovary in the sexual maturity period. |
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