| 张国鑫,赵跃斌,史雅静,等.“针药结合”对早期糖尿病视网膜病变大鼠血 -视网膜屏障的保护作用及机制研究[J].安徽医药,2026,30(10):2114-2120. |
| “针药结合”对早期糖尿病视网膜病变大鼠血 -视网膜屏障的保护作用及机制研究 |
| Protective effect and mechanism of "acupuncture combined with medication" on the blood-retinal barrier in rats with early diabetic retinopathy |
| |
| DOI：10.3969/j.issn.1009-6469.2026.10.039 |
| 中文关键词: 糖尿病视网膜病变 电针疗法 辛伐他汀 “针药结合” 大鼠 血 -视网膜屏障 血管内皮生长因子 血管生成素 -1 |
| 英文关键词: Diabetic retinopathy Electro-acupuncture therapy barrier Vascular endothelial growth factor Angiopoietin-1 |
| 基金项目:山西省中医药管理局项目( 2024ZYY2C133)；太原市科学技术局国家区域医疗中心科技创新计划项目( 202259) |
| 作者 | 单位 | E-mail | | 张国鑫 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | | | 赵跃斌 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | | | 史雅静 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | | | 王单峰 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | | | 渠鹏霞 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | | | 张莉 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | | | 张晶晶 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | | | 王文燕 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | | | 张晋峰 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | | | 贺美芳 | 山西医科大学第九临床医学院、太原市中心医院内分泌科,山西太原 030000 | 717120015@qq.com |
|
| 摘要点击次数: 78 |
| 全文下载次数: 22 |
| 中文摘要: |
| 目的探讨“针药结合”对糖尿病视网膜病变大鼠血 -视网膜屏障的保护作用及机制研究。方法研究时间为 2023年 10月至 2024年 4月。将 50只 SPF级 SD大鼠适应性饲养 7d后称体质量，依据体质量采用随机数字表法分为正常组、模型组、针刺组、药物组、针药组，建模成功后，正常组、模型组分别进行双蒸水灌胃和抓取、固定但不做针刺(假针刺)，针刺组予以电针干预 +双蒸水，药物组予以辛伐他汀药液灌胃 +假针刺，针药组予以电针干预 +辛伐他汀药液灌胃。检测各组大鼠血糖水平和血脂四项； HE染色观察大鼠视网膜病理变化；酶联免疫吸附分析(ELISA)检测血清内皮素 -1(ET-1)一氧化氮(NO)及氧化型低密度脂蛋白(OXLDL)的含量，蛋白质印迹法检测各组视网膜组织中血管内皮细胞因子(VEGF)和血管生、成素 -1(Ang-1)蛋白表达水平，逆转录聚合酶链式反应法(RT-PCR)测定各组视网膜组织中 VEGF和 Ang-1mRNA表达水平。结果与正常组相比，模型组和各治疗组的空腹血糖(GLU)糖化血清蛋白(GSP)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、ET-1、OXLDL的含量和视网膜组织中 VEGF、Ang-1蛋白及mR、NA表达水平均升高；与模型组相比，各治疗组的血糖、血脂水平以及视网膜组织中 VEGF、Ang-1蛋白 |
| 英文摘要: |
| Objective To explore the protective effect and mechanism of acupuncture combined with medication on the blood-retinal barrier in rats with diabetic retinopathy.Methods The research period was from October 2023 to April 2024. A total of 50 SPF-grade Sprague-Dawley (SD) rats were adaptively fed for 7 days and then weighed. Based on body weight, the rats were randomly divided into 5groups using a random number table: normal group, model group, acupuncture group, medication group, and acupuncture combinedwith medication group. After successful modeling, the normal group and model group were given double-distilled water by gavage andwere handled and fixed without acupuncture (sham acupuncture), the acupuncture group electroacupuncture intervention plus double-distilled water, the medication group simvastatin solution by gavage plus sham acupuncture, and the acupuncture combined with medi-cation group electroacupuncture intervention plus simvastatin solution by gavage. Blood glucose levels and blood lipids parameterswere detected in each group. The retinal pathological changes in each group were observed by hematoxylin-eosin (HE) staining. Serum levels of endothelin-1 (ET-1), nitric oxide (NO) and oxidized low-density lipoprotein (OXLDL) were measured by enzyme-linked immu-nosorbent assay (ELISA). The expression levels of vascular endothelial cytokine (VEGF) and angiopoietin-1 (Ang-1) in retinal tissues of each group were detected by Western blotting, and mRNA expression levels of VEGF and Ang-1 in retinal tissues of each group were determined by reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with normal group, model group and alltreatment groups showed elevated levels of fasting glucose (GLU), glycosylated serum protein (GSP), total cholesterol (TC), low-density lipoprotein cholesterol(LDL-C), ET-1, OXLDL, as well as increased VEGF and Ang-1 protein and mRNA expression in retinal tissues.Compared with model group, all treatment groups showed decreased blood glucose, lipid levels, and VEGF and Ang-1 protein in retinaltissues [(0.51±0.15), (0.34±0.07), (0.33±0.04), (0.26±0.03), respectively] as well as decreased mRNA expression. The medication groupand the acupuncture combined with medication group showed significant decreases in TC, LDL-C, ET-1 and OXLDL (P<0.05), while the acupuncture combined with medication group showed significant increases in NO and high-density lipoprotein cholesterol (HDL-C) levels (P<0.05). HE staining revealed that the acupuncture combined with medication group exhibited more significant improvement inthe ganglion cell layer of retinal tissues, with cells arranged in a double-layer pattern.Conclusion Acupuncture combined with medi-cation can regulate the metabolism of blood glucose and blood lipids, and downregulate the expression levels of VEGF, Ang-1 protein and mRNA in retinal tissues, which is considered to be one of the possible mechanisms underlying its protective effect. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
