| 巢蕾,曹雨诞,陈佩东.黄芩汤抗TNBS诱导的小鼠溃疡性结肠炎活性研究[J].安徽医药,2015,19(12):2292-2295. |
| 黄芩汤抗TNBS诱导的小鼠溃疡性结肠炎活性研究 |
| Effect of huangqin decoction on TNBS induced ulcerative colitis in mice |
| 投稿时间：2015-08-20 |
| DOI： |
| 中文关键词: 溃疡性结肠炎 黄芩汤 髓过氧化物酶 液质联用 2,4,6-三硝基苯磺酸钠 |
| 英文关键词: ulcerative colitis huangqin decoction MPO HPLC/MS 2,4,6-trinitrobenzenesulfonic acid |
| 基金项目:南京中医药大学风险基金(No CX201202) |
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| 中文摘要: |
| 目的 研究黄芩汤水煎液中主要成分及对2,4,6-三硝基苯磺酸钠 (TNBS)造模balb/c小鼠的溃疡性结肠炎的作用。方法 HPLC-MS法分析水煎液成分。雄性balb/c 小鼠随机分为空白对照组,TNBS模型组,柳氮磺嘧啶阳性对照组(500 mg·kg-1),黄芩汤水煎液1,2,4 g·kg-1治疗组。TNBS造模7 d后,研究各组小鼠的结肠病理评分、微观评分、体质量、小鼠结肠长度和血清髓过氧化物酶(MPO)。结果 与标准品对照,黄芩汤水煎液中含有复方中主要成分,黄芩汤水煎液可使TNBS造模balb/c小鼠的体质量明显增加,改善身体的一般状况和小鼠结肠黏膜的损伤,下调MPO。结论 黄芩汤水煎液中含有各药物的主要成分,对TNBS诱导的小鼠溃疡性结肠炎具有较好的治疗作用。 |
| 英文摘要: |
| Objective To investigate the main constituents and the effects of Huangqin decoction on 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced colitis in mice. Methods The decoction was analyzed by HPLC-MS, and the animals were randomly divided into 6 groups(n=10):normal control, TNBS control, positive control(500 mg·kg-1) and Huangqin decoction groups with 1,2,4 g·kg-1 respectively. 7 days after the induction of colitis with TNBS, the effects were studied by pathological score, histological analysis, body loss, colon length as well as by determination of MPO.Results The decoction had the main compounds of the plants. After the treatment with the decoction, the body weight and colon length were increased, the microscopic and macroscopic score were lowered, and the level of serum MPO activity was markedly decreased. Conclusion Hangqin decoction has the main constituents from various plants, and is effective in the treatment of colitis induced by TNBS. |
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