| 陈晓亮,汪旭.栀芩合剂质量控制方法的研究[J].安徽医药,2017,21(1):38-41. |
| 栀芩合剂质量控制方法的研究 |
| Quality control method of zhiqin mixture |
| 投稿时间：2016-08-18 |
| DOI： |
| 中文关键词: 栀芩合剂 质量标准 薄层色谱法 高效液相色谱法 黄芩苷 |
| 英文关键词: Zhiqin mixture Quality sttandard TLC HPLC Baicalin |
| 基金项目: |
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| 摘要点击次数: 2232 |
| 全文下载次数: 635 |
| 中文摘要: |
| 目的 提高并完善非标准制剂栀芩合剂质量标准。方法 采用薄层色谱法(TLC)对栀芩合剂中栀子、黄柏、黄芩的定性鉴别；采用高效液相色谱法(HPLC)测定栀芩合剂中黄芩苷的含量。色谱柱:Diamonsil C18 (250 mm×4.6 mm,5 μm) ,以甲醇-0.1%磷酸溶液(45∶55)为流动相,流速为1.0 mL·min-1,检测波长为280 nm。结果 在TLC定性鉴别中,供试品在与对照药材(或对照品)相应位置上出现相对应的特征斑点,阴性对照在相对应位置无干扰,专属性强；HPLC法可以测定黄芩苷的含量,黄芩苷的线性范围为0.096 4～0.867 6 μg (r=1),平均加样回收率为99.08%(n=9,RSD=1.17%)。结论 该质量标准确保栀芩合剂快速定性、定量分析,实验方法简单、重复性良好、结果稳定、可靠,能够用于栀芩合剂的质量控制。 |
| 英文摘要: |
| Objective To improve and complete the quality standards of the off-standard preparationof zhiqin mixture. Methods TLC was used to make qualitative identification of gardenia,golden cypress and Scutellaria baicalensis in zhiqin mixture;HPLC was used to determine the content of the baicalin in zhiqin mixture.Diamonsil C18 column (250 mm×4.6 mm,5 μm) was adopted,with the mixture of methanol-0.1% phosphoric acid (45∶55) as the mobile phase.The flow rate was set at 1.0 mL·min-1,and the wavelength for detection was 280 nm. Results In the qualitative identification of TLC,the test samples had correspondent characteristic spots in the locations in accordant with that in contrasted medicines(or contrasted articles),while the negative control was not disturbed in the correspondent locations,which manifested strong specificity.The content of baicalin was determined by HPLC,the linearity range was good within 0.096 4～0.867 6 μg (r=1),and the average recovery was 99.08%(n=9,RSD=1.17%). Conclusions The method to guarantee the quick qualitative analysis and quantitative analysis of Zhiqin mixture by establishing the quality standard is simple,repeatible,stable and reliable,which can be used for the quality control of zhiqin mixture. |
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