| 马木提江·阿巴拜克热,陈慧,刘学.橡皮筋挂线手术方法制备复杂性肛瘘大动物模型与评估[J].安徽医药,2017,21(3):458-461. |
| 橡皮筋挂线手术方法制备复杂性肛瘘大动物模型与评估 |
| Establishment and evaluation of large animal model of complex anal fistula using rubber ligation surgery |
| 投稿时间：2016-11-01 |
| DOI： |
| 中文关键词: 直肠瘘 挂线法 磁共振成像 猪 |
| 英文关键词: Rectal fistula Thread-ligating therapy Magnetic resonance imaging Swine |
| 基金项目:新疆维吾尔自治区区域协同创新专项(科技援疆项目)基金资助(2016E02063) |
|
| 摘要点击次数: 2020 |
| 全文下载次数: 616 |
| 中文摘要: |
| 目的 制备和评估橡皮筋挂线手术方式的大动物肛瘘模型。方法 选择15头猪(体质量35~42 kg)随机数字表法分成两组,实验组(8头)全麻下用橡皮筋挂线手术方法建立24个肛瘘瘘管管道,对照组(7头)全麻下用人工损伤方法建立21个管道。30 d后,所有动物进行MRI检查,实验组取出橡皮筋,两组接受临床和组织病理学评估。结果 实验组与对照组在体质量、性别、瘘管数量等一般特征上对比差异无统计学意义(P>0.05)。实验后所有动物均存活。实验组24个瘘管中,瘘管不愈合者(符合临床肛瘘者)18个,经核磁共振证实在高信号状态下可清晰呈现典型瘘道结构形态者15个,经病理学确认固定的瘘管样结构者14个,临床造模成功率75.00%。对照组21个瘘管中,15个管道完全愈合,6个不愈合(符合临床肛瘘者),经核磁共振证实在高信号状态下可清晰呈现典型瘘道结构形态者5个,经病理学确认固定的瘘管样结构者4个,临床造模成功率28.57%。 造模成功率比较,实验组显著优于对照组(P<0.05)。结论 橡皮筋挂线手术方法建立肛瘘模型稳定可靠,磁共振成像及组织学证实,实验组挂线手术方法建立的大动物瘘管具有出丰富的肉芽组织,大动物肛瘘模型可以进一步用于研究肛瘘的诊断与治疗领域。 |
| 英文摘要: |
| Objective To create and evaluate an experimental porcine model of fistula-in-ano by using rubber ligation surgery. Methods Fifteen healthy porcine were assigned into two groups randomly.Experiment group animals received rubber ligation procedure and control group received artificial damage processes.Thirtydays later after each procedure,fistula model of all porcine were detected by MRI imaging and pathological examination. Results All animals were alive.Baseline data of two groups were not significant statistically.Clinical success rate of animal model on experiment group was 75.00% (18 fistulas were confirmed in clinical,15 fistulas were confirmed by MRI,and 14 fistulas were confirmed by pathology).Clinical success rate of porcine fistula model of control group was 28.57% (6 fistulas were confirmed in clinical,5 fistulas were confirmed by MRI,and 4 fistulas were confirmed by pathology).Experimental group was significantly better than control group (P<0.05). Conclusions Operation method with rubber ligation can establish reliable fistula model.Model fistula had rich granulation tissue through the confirmation of MRI and pathology.Animal models of anal fistula can be used for the diagnosis and treatment research of anal fistula. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
