| 洪强,何胜利.高效液相色谱波长切换法同时测定甲亢灵片中4种成分的含量[J].安徽医药,2017,21(4):639-642. |
| 高效液相色谱波长切换法同时测定甲亢灵片中4种成分的含量 |
| Simultaneous determination of four ingredients in Jiakangling tablets by HPLC wavelength switching method |
| 投稿时间：2016-11-30 |
| DOI： |
| 中文关键词: 高效液相色谱法 甲亢灵片 去甲蟛蜞菊内酯 蟛蜞菊内酯 乌索酸 迷迭香酸 |
| 英文关键词: HPLC method Jiakangling Pian Isodemethy wedelolactonel Wedelolac-tone Ursolic acid Rosmarinic acid |
| 基金项目: |
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| 摘要点击次数: 2273 |
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| 中文摘要: |
| 目的 建立高效液相色谱(HPLC)波长切换法,同时测定甲亢灵片中去甲蟛蜞菊内酯、蟛蜞菊内酯、乌索酸和迷迭香酸含量。方法 采用Kromasil C18色谱柱(250 mm×4.6 mm,5 μm),流动相为甲醇(A)-0.5%冰醋酸溶液(B),梯度洗脱；柱温为30 ℃；进样量为10 μL；流速为1.0 mL·min-1；去甲蟛蜞菊内酯和蟛蜞菊内酯检测波长为λ1=351 nm,乌索酸检测波长为λ2=210 nm,迷迭香酸检测波长为λ3=330 nm。结果 去甲蟛蜞菊内酯、蟛蜞菊内酯、乌索酸和迷迭香酸在35 min内得到较好的分离,在给定浓度范围内与峰面积呈现良好线性,分别为5.90～118.00 mg·L-1(r=0.999 5)、5.37～107.40 mg·L-1(r=0.999 7)、5.04～100.80 mg·L-1(r=0.999 6)、7.65～153.00 mg·L-1(r=0.999 3)；平均加样回收率(n=6)和RSD值分别为98.6％(1.39％)、96.8％(0.83％)、99.2％(1.02％)、97.4％(0.85％)。结论 建立的HPLC方法可同时测定甲亢灵片中去甲蟛蜞菊内酯、蟛蜞菊内酯、乌索酸和迷迭香酸含量,方法简便、重现性好、灵敏度高,可用于甲亢灵片中去甲蟛蜞菊内酯、蟛蜞菊内酯、乌索酸和迷迭香酸的含量测定。 |
| 英文摘要: |
| Objective To establish an HPLC method for simultaneously determining the contents of isodemethyl wedelolactone,wedelolac-tone,ursolic acid and rosmarinic acid in Jiakangling tablets.Methods A stable HPLC method was established and the chromatography method was accomplished on a Kromasil C18 column (250 mm×4.6 mm,5 μm),with a mobile phase consisting of methanol (A)-0.5% acetic acid (B) with gradient elution;the column temperature was at 30 ℃;all the injection volume was 10 μL;the flow rate was 1.0 mL·min-1;isodemethyl wedelolactone and wedelolac-tone were detected at 351 nm,ursolic acid was detected at 210 nm,rosmarinic acid was detected at 330 nm.Results Isodemethyl wedelolactone,wedelolac-tone,ursolic acid and rosmarinic acid had good separation in 35 min.They showed good linearity in the given concentration range at 5.90-118.00 mg·L-1 (r=0.999 5),5.37-107.40 mg·L-1 (r=0.999 7),5.04-100.80 mg·L-1 (r=0.999 6),7.65-153.00 mg·L-1 (r=0.999 3),respectively.The average recoveries (n=6) and RSD were 98.6％(1.39％),96.8％(0.83％),99.2％(1.02％),97.4％ (0.85％),respectively. Conclusions This HPLC method could simultaneously determine the contents of isodemethyl wedelolactone,wedelolac-tone,ursolic acid and rosmarinic acid in Jiakangling tablets.The method is simple,sensitive and reliable,which can be used as a quantitative analysis method of isodemethyl wedelolactone,wedelolac-tone,ursolic acid and rosmarinic acid for Jiakangling tablets. |
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