| 赵春苗,程小刚,余擎.强酸性电解质水对饥饿态粪肠球菌生物膜的杀灭作用研究[J].安徽医药,2019,23(12):2424-2427. |
| 强酸性电解质水对饥饿态粪肠球菌生物膜的杀灭作用研究 |
| Investigation of the killing effect of strong acid electrolytic water on biofilm of enterococcus faecalis in starvation phase |
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| DOI：10.3969/j.issn.1009?6469.2019.12.023 |
| 中文关键词: 粪肠球菌 生物膜 饥饿 次氯酸 电解 根管冲洗剂 强酸性电解质水 杀菌作用 |
| 英文关键词: Enterococcus faecalis Biofilm Starvation Hypochlorous acid Electrolysis Root canal irrigants Strongaid electrolytic water Bactericidal efficiency |
| 基金项目:2018年度宝鸡市卫生健康委员会计划立项课题(2018?49) |
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| 中文摘要: |
| 目的观察强酸性电解质水(SAEW)对饥饿态粪肠球菌生物膜的杀菌作用。方法将 1.5×109CFU/mL的粪肠球菌 4℃低温离心、 PBS溶液溶解，饥饿诱导形成饥饿态粪肠球菌生物膜，用 SAEW、5.25%次氯酸钠(阳性对照)和 0.9%氯化钠溶液(NS，阴性对照)分别处理 1、5、10 min后，分别用活菌计数法和扫描电子显微镜(SEM)两种方法观察 SAEW对饥饿态粪肠球菌生物膜的杀灭作用。结果活菌计数法显示 SAEW对饥饿态粪肠球菌生物膜中的细菌表现出了有效的杀灭作用，且处理 10 min时的细菌存活率(5.31±0.74)%与 5.25%次氯酸钠的(4.06±1.44)%差异无统计学意义(P＞0.05)即杀菌作用接近。 SEM观察结果显示，随着 NS处理时间的延长细菌细胞皱缩失水现象加重； SAEW和 5.25%次氯酸钠处理后不，仅有严重的皱缩失水 |
| 英文摘要: |
| Objective To evaluate the bactericidal effect of strong acid electrolytic water(SAEW)on biofilm of enterococcus faeca? lis in starvation phase.Methods Enterococcus faecalis dilution(1.5×109 CFU/mL)was centrifuged at 4 ℃ and dissolved in PBSsolution.Enterococcus faecalis in starvation phase were incubated to develop bacterial biofilms.Then the biofilms were individuallytreated with SAEW，sodium hypochlorite(NaClO)， and normal saline for 1，5，and 10 min，respectively.Colony formation units(CFUs)counts and scanning electron microscopy(SEM)were used to investigate the bactericidal efficiency of SAEW on starved enterococcus faecalis biofilm.Results SAEW showed an effective killing effect on starved enterococcus faecalis biofilm.There wasno significant difference in bacterial survival rate between SAEW(5.31±0.74)% and 5.25% NaClO(4.06±1.44)% at 10 min(P＞0.05).That is，the killing effect is close.The results of SEM observation showed that the shrinkage and water loss of bacterialcells increased with the prolongation of NS treatment time.After treatment with SAEW and 5.25% NaClO，there was not only seri? ous shrinkage and water loss，but also dissolution and destruction of bacterial cells，perforation and fragment changes on the surface of cell membrane，and the destruction of bacterial cells became more serious with the prolongation of treatment time.Conclusion SAEW has effective bactericidal efficiency to starved enterococcus faecalis biofilm. |
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