| 杜晖,任静,郑妍.高效液相色谱法同时测定莨菪浸膏片中的 4种活性组分[J].安徽医药,2020,24(4):674-676. |
| 高效液相色谱法同时测定莨菪浸膏片中的 4种活性组分 |
| The simultaneous determination of four bioactive components in belladonna extract tablets by HPLC |
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| DOI：10.3969/j.issn.1009?6469.2020.04.009 |
| 中文关键词: 色谱法，高压液相 莨菪浸膏片 /分析 东莨菪碱 山莨菪碱 阿托品 东莨菪内酯 |
| 英文关键词: Chromatography,high pressure liquid Belladonna extract tablet/analysis Scopolamine Anisodamine Atropinesulphate Atropine sulphate |
| 基金项目:陕西省创新能力支撑计划( 2018KJXX?089)；陕西省中医药管理局科研项目( 13?ZY005) |
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| 中文摘要: |
| 目的建立高效液相色谱法( HPLC)同时测定莨菪浸膏片中东莨菪碱、山莨菪碱、硫酸阿托品和东莨菪内酯的含量。方法采用 Welch C18(150 mm×4.6 mm，5 μm)色谱柱，甲醇 -0.05%磷酸溶液梯度洗脱，流速 1.0 mL/min，进样量 20 μL，检测波长 215 nm。结果 4种组分分别在 0.501～20.059 μg/mL，0.988～19.761 μg/mL，0.468～9.361 μg/mL和 0.502～10.030 μg/mL浓度范围内线性关系良好，平均回收率在 98.3%～100.0%之间， RSD小于 2.8%。结论该方法简单准确，灵敏度高，可用于莨菪浸膏片真伪辨析和质量控制。 |
| 英文摘要: |
| Objective To establish a high performance liquid chromatography(HPLC)method for simultaneous determination of scopolamine，anisodamine，atropine sulphate and atropine sulphate in belladonna extract tablets.Methods HPLC separations were carried out on a Welch C18 column(150 mm×4.6 mm，5 μm)with a linear gradient elution using methanol and 0.05% phosphoricacid at a flow rate of 1.0 mL/min.The injection volume was 20 μL and the detection wavelength was 215 nm.Results Good linear relationships between the peak area and concentration were found in the ranges of 0.501～20.059 μg/mL，0.988～19.761 μg/mL，0.468～9.361 μg/mL and 0.502～10.030 μg/mL for four components，respectively.The average recoveries were in the ranges of 98.3%～100.0% with the RSD less than 2.8%.Conclusion The method is simple，accurate and sensitive.It can be used for distin? guishing the false and quality control of the belladonna extract tablets. |
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