| 陈哲,于康英,周鸣鸣.血管紧张素Ⅱ与血管平滑肌细胞中自噬水平的关系及机制研究[J].安徽医药,2020,24(5):947-951. |
| 血管紧张素Ⅱ与血管平滑肌细胞中自噬水平的关系及机制研究 |
| Study on the relationship and mechanism of autophagy in vascular smooth machine cells and Ang Ⅱ |
| |
| DOI：10.3969/j.issn.1009?6469.2020.05.025 |
| 中文关键词: 血管紧张素Ⅱ 肌细胞，平滑肌 自噬 受体，血管紧张素， 2型 西罗莫司 |
| 英文关键词: Angiotensin Ⅱ Myocytes,smooth muscle Autophagy Receptor,angiotensin,type 2 Sirolimus |
| 基金项目:江苏省高等学校自然科学基金面上项目( 17KJD310004) |
|
| 摘要点击次数: 2153 |
| 全文下载次数: 1059 |
| 中文摘要: |
| 目的研究血管紧张素 Ⅱ(Ang Ⅱ)是否能够引起血管平滑肌细胞( VSMC)自噬，探讨其相关作用机制。方法以 VSMC为研究对象，设立组别分别为空白组、 Ang Ⅱ组、自噬抑制剂( 3?MA)组、自噬促进剂雷帕霉素( Rap)组、血管紧张素 Ⅱ(AT1)受体抑制剂( ARB)组、 3?MA+Ang Ⅱ组、 Rap+Ang Ⅱ组、 ARB+Ang Ⅱ组，采用四唑盐( MTT)比色法检测细胞存活率，蛋白质免疫印迹法检测自噬相关蛋白脂化和膜相关蛋白( LC3 Ⅱ)表达的变化，免疫荧光法检测自噬小体数目的变化。结果实验中空白组吸光度为( 0.159±0.001)Ang Ⅱ组吸光度为( 0.151±0.003)，相比空白组，加入 Ang Ⅱ药物对细胞存活率变化不明显，差异无统计学意义(P＞0.05)。蛋白，质免疫印迹结果显示加入 Ang Ⅱ促进 VSMC的 LC3?Ⅱ表达量增多，呈现时间依赖性、浓度依赖性，表明 Ang Ⅱ促进 VSMC自噬发生。 3?MA对 Ang Ⅱ(10-7 mol/L，24 h)引起的 LC?3Ⅱ表达量增多起到抑制作用， Rap对 AngⅡ(10-7 mol/L，24 h)引起的 LC?3Ⅱ表达量增多起到叠加作用。 ARB对 Ang Ⅱ(10-7 mol/L，24 h)引起的 LC?3Ⅱ表达量增多起到抑制作用，表明自噬是由 Ang Ⅱ通过 ATI受体引起的。结论 Ang Ⅱ通过 AT1受体引起血管平滑肌细胞发生自噬。 |
| 英文摘要: |
| Objective To study if angiotensin Ⅱ(Ang Ⅱ)is able to cause autophagy in vascular smooth muscle cells(VSMC) and the related mechanisms of autophagy.Methods The VSMC was adopted as the research object.The establishment of groups were the control group，the Ang Ⅱ group，the 3?MA+Ang Ⅱ group，the Rap+ Ang Ⅱ group，the ARB+ Ang Ⅱ group，the 3?MA(autophagy inhibitor)group，the rapamycin(autophagy accelerator)group，the Angiotensin Ⅱ type 1 receptor blocker(ARB，AT1 receptor inhibitors)group.MTT detected the cell viability，western blot detected the changes of the expression of autophagy?related proteins LC3?Ⅱ，immunofluorescence detected the changes of the number of autophagic bodies.Results MTT results showed that compared to the control group，adding Ang Ⅱ drugs have little effect on cell survival，which suggested that Ang Ⅱ did not affectcell survival.Western blot showed that adding Ang Ⅱ could increase VSMC expression of LC3?Ⅱ，exhibiting a time?dependent， concentration?dependent manner，which suggested that Ang Ⅱ can promote VSMC autophagy.3?MA play an inhibitory effect on Ang Ⅱ(10-7 mol/L，24 h)caused increased expression of LC?3Ⅱ.Rapamycin play an overlay effect on ANG Ⅱ(10-7 mol/L，24 h) caused increased expression of LC?3Ⅱ.ARB play an inhibitory effect on Ang Ⅱ(10-7 mol/L，24 h)caused increased expression of LC?3Ⅱ，suggesting that Ang Ⅱ induced autophagy through ATI receptor.Conclusion Ang Ⅱ induces autophagy in vascular smooth muscle cells via AT1 receptor. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
