| 徐子涵,商玮,赵智明,等.五痹汤对大鼠佐剂性关节炎的疗效及肿瘤坏死因子 α、白细胞介素 1β水平的影响[J].安徽医药,2020,24(6):1240-1244. |
| 五痹汤对大鼠佐剂性关节炎的疗效及肿瘤坏死因子 α、白细胞介素 1β水平的影响 |
| Efficacy of Wubi decoction on adjuvant arthritis rats and its effects on the levels of TNF?α and IL?1 |
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| DOI：10.3969/j.issn.1009?6469.2020.06.045 |
| 中文关键词: 关节炎，实验性 关节炎，类风湿 肿瘤坏死因子 α 白细胞介素 1β 甲氨蝶呤 大鼠， Sprague?Dawley 五痹汤 |
| 英文关键词: Arthritis,experimental Arthritis,rheumatoid Tumor necrosis factor?alpha Interleukin?1beta Methotrexate Rats,Sprague?Dawley Wubi decoction |
| 基金项目:军队系统国家中医药管理局重点专科(专病)建设项目( 2007ZDZB001) |
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| 中文摘要: |
| 目的观察五痹汤对大鼠佐剂性关节炎的治疗效果及血清和关节滑液肿瘤坏死因子 α(TNF?α)、白细胞介素 1β(IL?1β)水平的影响。方法将 40只雄性 SD大鼠采用随机数字表法分为四组:健康组、模型组、五痹汤组和甲氨蝶呤组，每组各 10只。除健康组外，其余三组大鼠均建立佐剂性关节炎模型，于造模 9d后给药，未用药的实验日给予 0.9%氯化钠溶液 2毫升 /只灌胃，连续用药 14 d。观察大鼠足爪肿胀度、关节炎症程度及踝关节病理，酶联免疫吸附剂测定( ELISA)检测血清和关节滑液 TNF?α、IL?1β水平。结果从 d21起五痹汤组大鼠足爪肿胀度与模型组相比有所减轻( P＜0.05)。五痹汤组与甲氨蝶呤组大鼠 AI评分明显低于模型组，从 d18起差异有统计学意义( P＜0.05)。与健康组比较，模型组大鼠踝关节病理评分明显增高( P＜ 0.05)；五痹汤组与甲氨蝶呤组大鼠踝关节病理评分均低于模型组(P＜0.05)。与健康组比较，模型组大鼠血清和关节滑液 TNF?α、 IL?1β的水平均升高( P＜0.05)；经五痹汤治疗后，大鼠血清 TNF?α、IL?1β水平为( 105.62±7.01)pg/mL、(19.10±3.85)pg/mL，与模型组大鼠( 165.54±21.81)pg/mL、(33.67±4.51)pg/mL相比均明显降低( P＜0.05)；大鼠关节滑液 TNF?α、IL?1β水平为( 22.04±5.30)pg/mL、(32.11±1.82)pg/mL，与模型组大鼠( 38.33±8.67)pg/mL、(43.90±3.21)pg/mL相比均明显降低( P＜0.05)。结论五痹汤可减轻佐剂性关节炎大鼠足爪肿胀，缓解关节炎症反应，改善踝关节病理评分，降低血清和关节滑液 TNF?α、IL?1β水平，对大鼠佐剂性关节炎具有治疗作用。 |
| 英文摘要: |
| Objective To observe the therapeutic effect of Wubi decoction on adjuvant arthritis in rats and to explore its influence on TNF?α and IL?1 in serum and joint fluid.Methods Totally 40 male SD rats were assigned into 4 groups using random number table method:Healthy group，Model group，Wubi decoction group and MTX group，with 10 rats in each group.Rat models of adjuvantarthritis were prepared with rats in all groups except for the normal group.Drugs would be given 9 days after modeling.Each rat wasgiven 2 mL of 0.9% sodium chloride solution on the untreated days.Each group was given medication continuously for 14 days.Footswelling，arthritic index and ankle joint pathology were detected.The levels of TNF?α and IL?1 in serum and joint fluid were detect?ed by enzyme linked immunosorbent assay(ELISA).Results From d21，the paw swelling degree of Wubi decoction group was sig? nificantly reduced in comparison with Model group(P＜0.05).The AI scores of Wubi decoction group and MTX group were signifi? cantly lower than those of Model group，and the difference was statistically significant from d18(P＜0.05).Compared with Normal group，the ankle joint pathological score of Model group was significantly higher(P＜0.05).However，the ankle joint pathological scores of Wubi decoction group and MTX group were lower than that of Model group(P＜0.05).Compared with Normal group，the levels of TNF?α and IL?1β in serum and joint fluid in Model group were significantly increased(P＜0.05).After treatment with Wu? bi decoction，the levels of TNF?α and IL?1β in serum were(105.62±7.01)pg/mL and(19.10±3.85)pg/mL，respectively，which were significantly lower than(165.54±21.81)pg/mL and(33.67±4.51)pg/mL in Model group(P＜0.05).The levels of TNF?α and IL?1β in joint fluid were(22.04±5.30)pg/mL and(32.11±1.82)pg/mL in Wubi decoction group，which were significantly lower than(38.33±8.67)pg/mL and(43.90±3.21)pg/mL in Model group(P＜0.05).Conclusion Wubi decoction can alleviate paw swelling in adjuvant arthritis rats，relieve joint inflammation，improve ankle joint pathological score，reduce the levels of TNF?α and IL?1β in serum and joint fluid，which has a therapeutic effect on adjuvant arthritis rats. |
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