| 伍超,杨旭,陈桦,等.糖尿病视网膜病变病人血清微小 RNA?29c、微小 RNA?194的表达[J].安徽医药,2020,24(10):2034-2038. |
| 糖尿病视网膜病变病人血清微小 RNA?29c、微小 RNA?194的表达 |
| Expressions of serum miR?29c and miR?194 in patients with diabetic retinopathy |
| |
| DOI：10.3969/j.issn.1009?6469.2020.10.030 |
| 中文关键词: 糖尿病视网膜病变/病因学 微小 RNA?29c 微小 RNA?194 血红蛋白 A，糖基化 血糖 血清 |
| 英文关键词: Diabetic retinopathy/ etiology microRNA?29c microRNA?194 Hemoglobin A,glycosylated Blood glucose Blood serum |
| 基金项目: |
|
| 摘要点击次数: 1437 |
| 全文下载次数: 484 |
| 中文摘要: |
| 目的检测微小 RNA?29c(miR?29c)和微小 RNA?194(miR?194)在糖尿病视网膜病变(DR)病人血清中的表达特点及其临床意义。方法通过实时荧光定量 PCR(qRT?PCR)检测在汉中市人民医院就诊的 98例 DR病人、 98名Ⅱ型糖尿病(T2DM)病人和 98例同期体检健康者(NC)血清中 miR?29c和 miR?194的表达水平，多因素 logistic回归分析影响 DR发生的危险因素。 Pearson相关分析 DR病人血清 miR?29c和 miR?194与各生化指标的相关性。结果 DR组、 DM组和 NC组总胆固醇(TC)、低密度脂蛋白胆固醇(LDL?C)、高密度脂蛋白胆固醇(HDL?C)、三酰甘油(TG)、踝臂指数(ABI)、脉搏波传导速度(PWV)水平比较，差异无统计学意义(P＞0.05)； DR组和 DM组血清中糖化血红蛋白(HbA1c)、空腹血糖(FPG)水平及 miR?29c表达水平分别为(9.40±1.61)%比(8.53±1.78)%、(9.41±1.76)比(8.42±1.67)mmol/L、(1.29±0.57)比(1.12±0.25)明显高于 NC组:(7.93±2.64)%、(6.41±1.62)mmol/L、(1.01±0.10)，DR组明显高于 DM组，而 DR组和 DM组 miR?194表达水平明显低于 NC组， DR组明显低于 DM组(P＜0.05)； DR病人血清中 miR?29c相对表达量与 HbA1c、FPG水平均呈正相关(P＜0.05)与血清 miR?194相对表达量负相关(P＜0.05)； DR病人 miR?194与 miR?29c相对表达量呈负相关(P＜0.05)；多因素 logistic归分析发现， FPG水平高、回，HbA1c水平高、 miR?29c水平高是 DR发生的独立危险因素(均 P＜0.05)miR?194水平高是 DR发生的保护因素(P＜0.05)。结论 DR病人血清中 miR?29c表达相对 DM病人和健康者上调， miR?194表达，下调， miR?29c和 miR?194与 DR的发生发展有关。 |
| 英文摘要: |
| Objective To detect the expressions and clinical significances of microRNA?29c(miR?29c)and microRNA?194(miR? 194)in serum of patients with diabetic retinopathy(DR).Methods The expression levels of miR?29c and miR?194 in serum of 98 patients with DR，98 patients with type 2 diabetes mellitus(T2DM)and 98 healthy subjects(NC)in Hanzhong People′s Hos? pital were detected by real?time fluorescence quantitative PCR(qRT?PCR)，and the risk factors of DR were analyzed by multivari?ate logistic regression.Pearson correlation analysis was used to analyze the correlations between serum miR?29c and miR?194 andbiochemical parameters in DR patients.Results There was no significant difference in total cholesterol(TC)，low density lipopro? tein cholesterol(LDL?C)，high density lipoprotein cholesterol(HDL?C)triglyceride(TG)，ankle brachial index(ABI)and pulse wave velocity(PWV)levels among DR，DMandNCgroups(P＞0.05).T，he serum levels of glycosylated hemoglobin(HbA1c)， fasting plasma glucose(FPG)and miR?29c in DR group and DM group were(9.40±1.61)vs.(8.53±1.78)%，(9.41±1.76)vs.(8.42±1.67)mmol/L，(1.29±0.57)vs.(1.12±0.25)，respectively，which were significantly higher than those in NC group(7.93±2.64)%，(6.41±1.62)mmol/L，(1.01±0.10)，DR group was significantly higher than that in DM group，while the level of miR?194 in DR group and DM group was significantly lower than that in NC group，and that in DR group was significantly lower than that in DM group(P＜0.05).The relative expression of miR?29c in serum of DR patients was positively correlated with HbA1c and FPG levels(P＜0.05)，and negatively correlated with the relative expression of miR?194 in serum(P＜0.05).There was a positive corre? lation between the relative expressions of miR?194 and miR?29c in DR patients(P＜0.05).Multivariate logistic regression analysis showed that high levels of FPG，HbA1c and miR?29c were independent risk factors for DR(all P＜0.05)，high level of miR?194 was protective factor for DR(P＜0.05).Conclusions The expression of miR?29c in serum of DR patients is up?regulated and theexpression of miR?194 is down?regulated compared with that of DM patients and healthy people，the expressions of miR?29c and miR?194 are related to the development of DR. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
