| 赵晓娟,赵永红,王淑梅,等.高效液相色谱法测定不同时间人血浆中帕拉米韦的浓度及临床观察[J].安徽医药,2021,25(2):243-246. |
| 高效液相色谱法测定不同时间人血浆中帕拉米韦的浓度及临床观察 |
| Determination the concentration of peramivir in human plasma at different time by HPLC and its clinical observation |
| |
| DOI：10.3969/j.issn.1009-6469.2021.02.007. |
| 中文关键词: 色谱法，高压液相 帕拉米韦 人血浆药物浓度 临床观察 |
| 英文关键词: Chromatography,high pressure liquid Peramivir Plasma drug concentration Clinical observation |
| 基金项目:河北省卫生和健康委员会科研基金项目( 20170573) |
|
| 摘要点击次数: 1513 |
| 全文下载次数: 390 |
| 中文摘要: |
| 目的测定帕拉米韦给药后不同时间人血浆中的药物浓度，观察病人用药后安全性及疗效，为临床使用帕拉米韦提供依据。方法高效液相色谱( HPLC)法测定帕拉米韦的血药浓度，以 5 mmol/L磷酸二氢钾溶液( 0.1%三乙胺) -乙腈为流动相，磷酸调 pH 5.0；检测波长 210 nm；流速 1.0 mL/min；用药后对病人进行安全性随访。结果血浆中无干扰测定的内源性物质，线性范围为 1~50μg/mL，最低定量限( LLOQ)为 1 μg/mL，回收率 102.5%~110.4%，日间精密度及稳定性均良好， 5例病人不同时间血浆中的帕拉米韦均可检出； 5例病人的发热缓解时间分别为 33、26、26、48、54 h，流感症状缓解时间分别为 89、>120、28、 48、54 h，发生 1例不良反应。结论 HPLC检测方法可靠，适用于人血浆中帕拉米韦的测定。帕拉米韦能快速缓解流感症状，虽然出现的不良事件为轻度，但也要监测其不良反应，以保证用药安全性及有效性。 |
| 英文摘要: |
| Objective To determine the concentration of drug in human plasma at different times after the administration of peramivir, and to observe the safety and efficacy of the drug after administration, and to provide a basis for clinical use of peramivir.Methods The plasma concentration of peramivir was determined by HPLC. The mobile phase was 5 mmol/L potassium dihydrogen phosphate solution (0.1% triethylamine)-acetonitrile, and adjusted pH to 5.0. The detection wavelength was 210 nm. The flow rate was 1.0 mL/min;all patients were followed up for safety.Results The endogenous substances in the plasma without interference were linear ranged from 1-50 μg/mL, the lowest limit of quantitation (LLOQ) was 1 μg/mL, and the recovery was 102.5%-110.4%. The precision and stability during the day were good. The patients in the plasma at different times of peramivir can be detected; five patients with fever relieftime were 33, 26, 26, 48, 54h; the flu symptom relief time was 89, >120, 28, 48, 54 h; 1 case of adverse reaction occurred.Conclusion The HPLC method was reliable and suitable for the determination of peramivir in human plasma. Peramivir can quickly relieve thesymptoms of flu, although the adverse events are mild, but monitor the adverse reactions to ensure the safety and effectiveness ofmedication. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
