| 王丽,周小勤,涂丹娜,等.尿液及血清骨膜蛋白的检测对早期诊断儿童紫癜性肾炎的价值[J].安徽医药,2021,25(2):258-260. |
| 尿液及血清骨膜蛋白的检测对早期诊断儿童紫癜性肾炎的价值 |
| Clinical value of urine and serum periostin for children with henoch schonlein purpura nephritis in early stage |
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| DOI：10.3969/j.issn.1009-6469.2021.02.011. |
| 中文关键词: 紫癜，过敏性 紫癜性肾炎 骨膜蛋白 儿童 |
| 英文关键词: Purpura,henoch-schonlein Henoch-Schonlein purpura nephritis Periostin Child |
| 基金项目:湖北省自然科学基金项目( 2015CFB660) |
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| 中文摘要: |
| 目的探讨尿液及血清骨膜蛋白的检测对早期诊断儿童紫癜性肾炎(HSPN)的价值。方法选取 2012年 6月至 2018年 6月在湖北省妇幼保健院就诊的 HSPN病儿 35例为 HSPN组，选取同期在湖北省妇幼保健院门诊健康体检的 35例儿童为健康对照组。采用酶联免疫吸附法( ELISA)测定并比较两组尿液及血清骨膜蛋白的表达水平，用 Medcalc软件绘制受试者工作特征(ROC)曲线评估骨膜蛋白对 HSPN早期诊断的价值。结果健康对照组与 HSPN组年龄比较，差异无统计学意义［(8.38±1.72)岁比(8.62±1.68)岁， t=1.629，P=0.072］；健康对照组与 HSPN组尿液骨膜蛋白表达水平［(31.91±1.78)pg/mL比(34.74±2.27)pg/mL］及血清骨膜蛋白表达水平［(40.15±7.46)pg/mL比(48.63±6.92)pg/mL］比较，均差异有统计学意义( P < 0.01)； ROC曲线分析结果显示 HSPN组尿液及血清骨膜蛋白表达水平曲线下面积(AUC)分别为 0.838与 0.778；相对血清，尿液骨膜蛋白表达水平的灵敏度和特异性较好，差异有统计学意义(Z=6.528，P < 0.001)。结论尿液骨膜蛋白检测是 HSPN早期诊断的敏感指标。 |
| 英文摘要: |
| Objective To investigate the relationship between Henoch-Schonlein purpura nephritis (HSPN) and periostin in children, and to provide scientific evidence for the study of HSPN in children.Methods A total of 35 children with HSPN who were admitted to Hubei Provincial Maternity and Child Health Hospital from June 2012 to June 2018 were enrolled as HSPN group. 35 childrenwho received physical examination in the outpatient health in Hubei Maternity and Child Health Hospital during the same period wereselected as the normal control group. Enzyme-linked immunosorbent assay (ELISA) was used to determine the level of periostin in urineand serum of the subjects. The expression levels of periostin in urine and serum of normal control group and HSPN group were compared, receiver operating characteristic curve (ROC curve) was calculated by Medcalc software to evaluate the value of periostein onHSPN.Results The study found that the age of the normal control group and the HSPN group showed no significant difference between the two groups [(8.38±1.72) vs. (8.62±1.68), t=1.629, P=0.072]. The difference of periplasmic protein expression in urine [(31.91±1.78) pg/mL vs. (34.74±2.27) pg/mL] and serum [(40.15±7.46) pg/mL vs. (48.63±6.92) pg/mL] between the two groups was statistically significant (P < 0.01); ROC curve analysis showed that the area under the curve of periostin expression in urine and serum(AUC) was 0.838 and 0.778, respectively. The sensitivity and specificity of periostin expression levels in children's urine were relatively good. The differences in the diagnostic value of urine and serum periostin for children with HSPN in early stage were statistically significant (Z=6.528, P < 0.001).Conclusion Urinary periostin is a sensitive indicator for HSPN-related studies. |
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