| 祁彦萍,王英,杨建敏.长链非编码 RNA GAS5、微小 RNA-21在多囊卵巢综合征中的表达及生物学意义[J].安徽医药,2021,25(2):304-308. |
| 长链非编码 RNA GAS5、微小 RNA-21在多囊卵巢综合征中的表达及生物学意义 |
| Expressions of LncRNA GAS5 and miR-21 in polycystic ovary syndrome and their biological significance |
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| DOI：10.3969/j.issn.1009-6469.2021.02.023. |
| 中文关键词: 多囊卵巢综合征 LncRNA GAS5 微小 RNA-21 卵巢颗粒细胞 增殖 凋亡 |
| 英文关键词: Polycystic ovary syndrome Long non-coding RNA GAS5 microRNA-21 Ovarian granulosa cells Proliferation Apoptosis |
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| 中文摘要: |
| 目的探讨长链非编码 RNA(LncRNA)GAS5与微小 RNA-21(miR-21)在多囊卵巢综合征( PCOS)中的表达及其对卵巢颗粒细胞增殖及凋亡的影响。方法收集 2017年 4月至 2018年 10月于南阳市中心医院就诊的 50例 PCOS病人为观察组，选取同时期于南阳市中心医院就诊的 45例非 PCOS病人为对照组，实时荧光定量聚合酶链式反应( qRT-PCR)检测对照组与观察组病人血清中 GAS5与 miR-21的表达水平。采用 Pearson法分析 PCOS病人中 GAS5与 miR-21表达的相关性。原代培养大鼠卵巢颗粒细胞，分别将 pcDNA、pcDNA-GAS5转染至卵泡颗粒细胞。 CCK-8检测细胞增殖；流式细胞术检测细胞凋亡率；双荧光素酶报告实验验证 GAS5与 miR-21的靶向关系。结果观察组病人血清中 GAS5的表达水平明显低于对照组［( 0.52±0.11)比( 1.00±0.12)P < 0.05］，miR-21的表达水平明显高于对照组［( 2.36±0.57)比( 1.01±0.10)P < 0.05］，GAS5与 miR-21呈负相关( r = ?0.310= 0.028)； TT、IL-18与 RGAS5呈负相关( P < 0.05)< 0.05)；与 pcDNA组相比， pcDNA-GAS5组细胞活力降低( P < 0.05)，细胞凋亡率升高［(36.49±5.21(8.52±1.20)，P < 0.05］；双荧光素酶报告实验证实 ，P，而与miR-21呈正相关(P，)比，GAS5可靶向结合 miR-21，并可负向调控 miR-21的表达。结论 GAS5过表达可能通过靶向调控 miR-21表达从而抑制卵巢颗粒细胞增殖，促进细胞凋亡。 |
| 英文摘要: |
| Objective To investigate the expression of LncRNA GAS5 and miR-21 in PCOS and their effects on the proliferation and apoptosis of ovarian granulosa cells.Methods qRT-PCR was used to detect the expression levels of GAS5 and miR-21 in the serum of patients in the control and observation groups.The correlation between GAS5 and miR-21 expression in PCOS patients was analyzed by Pearson method.Primary cultured rat ovarian granulosa cells were transfected with pcDNA, pcDNA-GAS5 into follicular granule cells,respectively.CCK-8 was used to detect cell proliferation.Apoptosis rate was measured by flow cytometry.The double luciferasereport experiment verified the targeting relationship between GAS5 and miR-21.Results The expression level of GAS5 in the serum of the observation group was significantly lower than that of the control group[(0.52±0.11) vs.(1.00±0.12),P < 0.05], and the expression level of miR-21 was significantly higher than that of the control group[(2.36±0.57) vs.(1.01±0.10),P < 0.05]. GAS5 was negatively correlated with miR-21 (r = ?0.310 , P = 0.028). GAS5 was negatively correlated with TT,IL-18 (P < 0.05), and miR-21 was positively correlated with TT,IL-18 (P < 0.05).Compared with the pcDNA group, the cell viability of the pcDNA-GAS5 group was significantly reduced (P < 0.05), the apoptosis rate was significantly increased [(36.49±5.21) vs.(8.52±1.20),P < 0.05]. The double luciferase reporting experiment confirmed that GAS5 can target miR-21 and negatively regulate miR-21 expression. Conclusion GAS5 overexpression may inhibit miR-21 expression by targeting to inhibit ovarian granulosa cell proliferation and promote apoptosis. |
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